Crystallization (Comment) | Organism |
---|---|
high resolution crystal structure of NovP as a binary complex with its desmethylated co-substrate, S-adenosyl-L-homocysteine. The structure includes a helical lid region that gates access to the co-substrate binding pocket, and an active centre that contains a 3-Asp putative metal-binding site. A further conserved Asp likely acts as the general base that initiates the reaction by deprotonating the 4-OH group of the noviose unit. Models suggest that NovP is unlikely to tolerate significant modifications at the noviose moiety, but could show increasing substrate promiscuity as a function of the distance of the modification from the methylation site. These observations could inform future attempts to utilise NovP for methylating a range of glycosylated compounds | Streptomyces niveus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
gel filtration | Streptomyces niveus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces niveus | Q9L9F2 | - |
- |
Purification (Comment) | Organism |
---|---|
N-terminally His-tagged enzyme | Streptomyces niveus |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Streptomyces niveus |
Synonyms | Comment | Organism |
---|---|---|
NovP | - |
Streptomyces niveus |