Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzene sulfonate | potent inhibition | Aspergillus terreus | |
iodoacetate | slight inhibition | Aspergillus terreus | |
additional information | no inhibition by metal chelating agents | Aspergillus terreus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00034 | - |
emodin | pH 8.0, 30°C | Aspergillus terreus | |
0.0041 | - |
S-adenosyl-L-methionine | pH 8.0, 30°C | Aspergillus terreus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Mg2+ or Mn2+ is not an absolute requirement, nor increases the enzyme activity | Aspergillus terreus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53600 | - |
6 * 53600, SDS-PAGE | Aspergillus terreus |
322000 | - |
gel filtration | Aspergillus terreus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + emodin | Aspergillus terreus | the enzyme is involved in biosynthesis of the seco-anthraquinone (+)-geodin | S-adenosyl-L-homocysteine + questin | - |
? | |
S-adenosyl-L-methionine + emodin | Aspergillus terreus IMI 16043 | the enzyme is involved in biosynthesis of the seco-anthraquinone (+)-geodin | S-adenosyl-L-homocysteine + questin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus terreus | - |
- |
- |
Aspergillus terreus IMI 16043 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus terreus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus terreus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.18 | - |
pH 8.0, 30°C | Aspergillus terreus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 1,3,8-trihydroxy-6-methoxy-9,10-anthraquinone | i.e. citreorosein, 22% of the activity compared to emodin | Aspergillus terreus | ? | - |
? | |
S-adenosyl-L-methionine + 1,3,8-trihydroxy-6-methoxy-9,10-anthraquinone | i.e. citreorosein, 22% of the activity compared to emodin | Aspergillus terreus IMI 16043 | ? | - |
? | |
S-adenosyl-L-methionine + 7-chloroemodin | 18% of the activity compared to emodin | Aspergillus terreus | ? | - |
? | |
S-adenosyl-L-methionine + 7-chloroemodin | 18% of the activity compared to emodin | Aspergillus terreus IMI 16043 | ? | - |
? | |
S-adenosyl-L-methionine + catenarin | 80% of the activity compared to emodin | Aspergillus terreus | ? | - |
? | |
S-adenosyl-L-methionine + catenarin | 80% of the activity compared to emodin | Aspergillus terreus IMI 16043 | ? | - |
? | |
S-adenosyl-L-methionine + emodin | - |
Aspergillus terreus | S-adenosyl-L-homocysteine + questin | - |
? | |
S-adenosyl-L-methionine + emodin | the enzyme is involved in biosynthesis of the seco-anthraquinone (+)-geodin | Aspergillus terreus | S-adenosyl-L-homocysteine + questin | - |
? | |
S-adenosyl-L-methionine + emodin | - |
Aspergillus terreus IMI 16043 | S-adenosyl-L-homocysteine + questin | - |
? | |
S-adenosyl-L-methionine + emodin | the enzyme is involved in biosynthesis of the seco-anthraquinone (+)-geodin | Aspergillus terreus IMI 16043 | S-adenosyl-L-homocysteine + questin | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 53600, SDS-PAGE | Aspergillus terreus |
Synonyms | Comment | Organism |
---|---|---|
EOMT | - |
Aspergillus terreus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Aspergillus terreus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Aspergillus terreus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.7 | 9 | half maximal activity at pH 5.7 and pH 9.0 | Aspergillus terreus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus terreus | isoelectric focusing | - |
4.4 |