Cloned (Comment) | Organism |
---|---|
hexahistidine-tagged enzyme is expressed in Saccharomyces cerevisiae | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe | Saccharomyces cerevisiae | the enzyme is involved in the biosynthesis of the tricyclic base wybutosine | S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-methyl]wyosine37 in tRNAPhe | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P53177 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe | the enzyme is involved in the biosynthesis of the tricyclic base wybutosine | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-methyl]wyosine37 in tRNAPhe | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TYW3 | - |
Saccharomyces cerevisiae |
YGL050w | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | in DELTATYW3 strain, tRNAPhe has two wybutosine intermediates, 7-(3-amino-3-carboxypropyl)-4-demethylwyosine37 (yW-86) and 4-demethylwyosine37 (yW-14). Recombinant TYW3 clearly methylated 7-(3-amino-3-carboxypropyl)-4-demethylwyosine37 in tRNA to yield 7-(3-amino-3-carboxypropyl)-4-methylwyosine37 in tRNA in S-adenosyl-L-methionine-dependent manner. 4-Demethylwyosine37 is not methylated by TYW3 | Saccharomyces cerevisiae |
physiological function | the enzyme is involved in the biosynthesis of the tricyclic base wybutosine | Saccharomyces cerevisiae |