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Literature summary for 2.1.1.280 extracted from

  • Lyi, S.M.; Heller, L.I.; Rutzke, M.; Welch, R.M.; Kochian, L.V.; Li, L.
    Molecular and biochemical characterization of the selenocysteine Se-methyltransferase gene and Se-methylselenocysteine synthesis in broccoli (2005), Plant Physiol., 138, 409-420.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.280

Cloned(Commentary)

Cloned (Comment) Organism
BoSMT overexpression in Escherichia coli strain MTD123 Brassica oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37900
-
 calculated from protein sequence Brassica oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + L-selenocysteine Brassica oleracea selenate-treated plants synthesize significantly more Se-methyl-L-selenocysteine S-adenosyl-L-homocysteine + Se-methyl-L-selenocysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Brassica oleracea Q4VNK0 var. italica
-

Source Tissue

Source Tissue Comment Organism Textmining
floret
-
 Brassica oleracea
-
leaf
-
 Brassica oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.001
-
 with 10 mM DL-homocysteine as methyl acceptor and [methyl-14C]adenosylmethionine as the methyl donor, pH 7.0 and 25°C Brassica oleracea
0.0011
-
 with 10 mM L-cysteine as methyl acceptor and [methyl-14C]adenosylmethionine as the methyl donor, pH 7.0 and 25°C Brassica oleracea
0.0018
-
 with 10 mM DL-cysteine as methyl acceptor and [methyl-14C]adenosylmethionine as the methyl donor, pH 7.0 and 25°C Brassica oleracea
0.0024
-
 with 0.5 mM L-selenocysteine as methyl acceptor and [methyl-14C]adenosylmethionine as the methyl donor, pH 7.0 and 25°C Brassica oleracea
0.0051
-
 with 0.5 mM DL-selenocysteine as methyl acceptor and [methyl-14C]adenosylmethionine as the methyl donor, pH 7.0 and 25°C Brassica oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information expression of BoSMT significantly enhances Se tolerance with an increased level of total Se accumulation in Escherichia coli Brassica oleracea ?
-
 ?
S-adenosyl-L-methionine + DL-cysteine
-
 Brassica oleracea ?
-
 ?
S-adenosyl-L-methionine + DL-homocysteine
-
 Brassica oleracea ?
-
 ?
S-adenosyl-L-methionine + DL-selenocysteine highest preference as methyl acceptor Brassica oleracea ?
-
 ?
S-adenosyl-L-methionine + L-cysteine
-
 Brassica oleracea S-adenosyl-L-homocysteine + S-methyl-L-cysteine
-
 ?
S-adenosyl-L-methionine + L-selenocysteine selenate-treated plants synthesize significantly more Se-methyl-L-selenocysteine Brassica oleracea S-adenosyl-L-homocysteine + Se-methyl-L-selenocysteine
-
 ?

Synonyms

Synonyms Comment Organism
BoSMT
-
 Brassica oleracea
SMT
-
 Brassica oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Brassica oleracea

Expression

Organism Comment Expression
Brassica oleracea concentrations higher than 40 microM show a negative effect on BoSMT mRNA accumulation. At very high selenate concentrations e.g. 100 microM, the expression of BoSMT mRNA substantially reduced down
Brassica oleracea high sulfate levels in growth medium significantly reduce accumulation of BoSMT transcripts in plants down
Brassica oleracea treating Brassica oleracea plants with selenate results in a rapid accumulation of BoSMT mRNA and reaches a maximum expression at selenate levels between 20 and 40 microM. This is in contrast to 40 microM selenite, which causes only a slight accumulation of the transcript up

General Information

General Information Comment Organism
metabolism SMT is the key enzyme for Se-methylselenocysteine synthesis Brassica oleracea
physiological function expression of BoSMT significantly enhances Se tolerance, high levels of Se accumulate in Broccoli plants exposed to selenate, but addition of high levels of 1 or 10 mM sulfate shows a strong inhibitory effect on Se accumulation Brassica oleracea