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Literature summary for 2.1.1.280 extracted from

  • Neuhierl, B.; Böck, A.
    On the mechanism of selenium tolerance in selenium-accumulating plants. Purification and characterization of a specific selenocysteine methyltransferase from cultured cells of Astragalus bisculatus (1996), Eur. J. Biochem., 239, 235-238.
    View publication on PubMed
Search for more literature for 2.1.1.280

Inhibitors

Inhibitors Comment Organism Structure
L-selenocysteine above 1 mM Astragalus bisulcatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 S-adenosyl-L-methionine pH 6.0, 30°C Astragalus bisulcatus
0.3
-
 DL-selenocysteine pH 6.0, 30°C Astragalus bisulcatus
0.7
-
 L-selenocysteine pH 6.0, 30°C Astragalus bisulcatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 gel filtration Astragalus bisulcatus
38000
-
 SDS-PAGE and gel filtration on a Superdex 75 column Astragalus bisulcatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-selenocysteine + S-adenosyl-L-methionine Astragalus bisulcatus the enzyme is suitable for specific detoxification of selenocysteine and preventing selenium from intrusion into downstream sulfur pathways. Plays a crucial role in conferring selenium tolerance Se-methyl-L-selenocysteine + S-adenosyl-L-homocysteine
-
 ?
S-methyl-L-methionine + L-selenocysteine Astragalus bisulcatus
-
 L-methionine + Se-methyl-L-selenocysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Astragalus bisulcatus P56707
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Astragalus bisulcatus
more than 2800fold purification by (NH4)2SO4 precipitation, anion-exchange chromatography and hydrophobic-interaction chromatography Astragalus bisulcatus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00971
-
-
 Astragalus bisulcatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-selenocysteine + S-adenosyl-L-methionine the stereochemical orientation of the alpha-amino group is not crucial. With DL-selenocysteine or DL-selenohomocysteine as substrates, the Km decreases. This leads to the overproportional increase of specific activity with DL-selenocysteine Astragalus bisulcatus Se-methyl-selenocysteine + S-adenosyl-L-homocysteine
-
 ?
L-selenocysteine + S-adenosyl-L-methionine the enzyme is suitable for specific detoxification of selenocysteine and preventing selenium from intrusion into downstream sulfur pathways. Plays a crucial role in conferring selenium tolerance Astragalus bisulcatus Se-methyl-L-selenocysteine + S-adenosyl-L-homocysteine
-
 ?
additional information methylation of L-cysteine can not be detected unless the concentration is raised to 10 mM. Under this condition, the reaction velocity is approximately 1% compared with methylation of L-selenocysteine. No activity with selenocysteamine and DL-homocysteine Astragalus bisulcatus ?
-
 ?
S-adenosyl-L-methionine + DL-homocysteine less than 1% relative activity with 10 mM DL-homocysteine compared to activity with 1 mM DL-selenocysteine set at 100% Astragalus bisulcatus ?
-
 ?
S-adenosyl-L-methionine + DL-selenocysteine 475% relative activity with 1 mM DL-selenocysteine compared to 1 mM L-selenocysteine activity set at 100% Astragalus bisulcatus ?
-
 ?
S-adenosyl-L-methionine + DL-selenohomocysteine 25% relative activity with 1 mM DL-selenohomocysteine compared to activity with 1 mM DL-selenocysteine set at 100% Astragalus bisulcatus ?
-
 ?
S-adenosyl-L-methionine + L-cysteine 1% relative activity with 10 mM L-cysteine compared to 1 mM L-selenocysteine activity set at 100% Astragalus bisulcatus S-methyl-L-cysteine + S-adenosyl-L-homocysteine
-
 ?
S-adenosyl-L-methionine + L-selenocysteine
-
 Astragalus bisulcatus Se-methyl-L-selenocysteine + S-adenosyl-L-homocysteine
-
 ?
S-adenosyl-L-methionine + selenocysteamine less than 1% relative activity with 1 mM selenocysteamine compared to 1 mM L-selenocysteine activity set at 100% Astragalus bisulcatus ?
-
 ?
S-methyl-L-methionine + L-selenocysteine
-
 Astragalus bisulcatus L-methionine + Se-methyl-L-selenocysteine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 38000, SDS-PAGE Astragalus bisulcatus

Synonyms

Synonyms Comment Organism
SEcMT
-
 Astragalus bisulcatus
selenocysteine methyltransferase
-
 Astragalus bisulcatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Astragalus bisulcatus

General Information

General Information Comment Organism
metabolism enzyme plays a crucial role in the detoxification of selenium in Astragalus bisulcatus Astragalus bisulcatus