Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.28 extracted from

  • Axelrod, J.
    Purification and properties of phenylethanolamine-N-methyltransferase (1962), J. Biol. Chem., 237, 1657-1660.
    View publication on PubMed
Search for more literature for 2.1.1.28

Inhibitors

Inhibitors Comment Organism Structure
PCMB
-
 Bos taurus
PCMB 0.01 mM, complete inhibition Macaca mulatta
PCMB
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 normetanephrine
-
 Macaca mulatta

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Cavia porcellus
-
-
-
Felis catus
-
-
-
Macaca mulatta
-
-
-
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Macaca mulatta

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland
-
 Rattus norvegicus
-
adrenal gland
-
 Bos taurus
-
adrenal gland
-
 Oryctolagus cuniculus
-
adrenal gland adrenal medulla has considerably more activity than the cortex Macaca mulatta
-
adrenal medulla
-
 Bos taurus
-
adrenal medulla
-
 Macaca mulatta
-
heart
-
 Rattus norvegicus
-
heart weak activity Oryctolagus cuniculus
-
heart weak activity Macaca mulatta
-

Storage Stability

Storage Stability Organism
-10°C, stable for at least 1 month Macaca mulatta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dichlorophenylethanolamine + S-adenosyl-L-methionine
-
 Oryctolagus cuniculus N-methyl-3,4-dichlorophenylethanolamine + S-adenosyl-L-homocysteine
-
 ?
3,4-dichlorophenylethanolamine + S-adenosyl-L-methionine
-
 Macaca mulatta N-methyl-3,4-dichlorophenylethanolamine + S-adenosyl-L-homocysteine
-
 ?
3,4-dichlorophenylethylenediamine + S-adenosyl-L-methionine
-
 Macaca mulatta ? + S-adenosyl-L-homocysteine
-
 ?
3,4-dihydroxynorephedrine + S-adenosyl-L-methionine 18% of the activity with normetanephrine Macaca mulatta 3,4-dihydroxyephedrine + S-adenosyl-L-homocysteine
-
 ?
3-chloro-4-hydroxyphenylethanolamine + S-adenosyl-L-methionine
-
 Macaca mulatta ? + S-adenosyl-L-homocysteine
-
 ?
4-hydroxynorephedrine + S-adenosyl-L-methionine 18% of the activity with normetanephrine Macaca mulatta 4-hydroxyephedrine + S-adenosyl-L-homocysteine
-
 ?
D-norepinephrine + S-adenosyl-L-methionine
-
 Oryctolagus cuniculus epinephrine + S-adenosyl-L-homocysteine
-
 ?
D-norepinephrine + S-adenosyl-L-methionine 15% of the activity with normetanephrine Macaca mulatta epinephrine + S-adenosyl-L-homocysteine
-
 ?
DL-m-hydroxyphenylethanolamine + S-adenosyl-L-methionine 60% of the activity with normetanephrine Macaca mulatta neosynephrine + S-adenosyl-L-homocysteine
-
 ?
DL-neo-synephrine + S-adenosyl-L-methionine 20% of the activity with normetanephrine Macaca mulatta 3-hydroxy-alpha-[(dimethylamine)methyl]benzenemethanol + S-adenosyl-L-homocysteine
-
 ?
DL-octopamine + S-adenosyl-L-methionine 18% of the activity with normetanephrine Macaca mulatta ? + S-adenosyl-L-homocysteine
-
 ?
L-norephedrine + S-adenosyl-L-methionine 14% of the activity with normetanephrine Macaca mulatta ephedrine + S-adenosyl-L-homocysteine
-
 ?
L-norepinephrine + S-adenosyl-L-methionine
-
 Oryctolagus cuniculus epinephrine + S-adenosyl-L-homocysteine
-
 ?
L-norepinephrine + S-adenosyl-L-methionine 21% of the activity with normetanephrine Macaca mulatta epinephrine + S-adenosyl-L-homocysteine
-
 ?
normetanephrine + S-adenosyl-L-methionine
-
 Bos taurus metanephrine + S-adenosyl-L-homocysteine
-
 ?
normetanephrine + S-adenosyl-L-methionine
-
 Macaca mulatta metanephrine + S-adenosyl-L-homocysteine
-
 ?
phenylethanolamine + S-adenosyl-L-methionine
-
 Bos taurus N-methylphenylethanolamine + S-adenosyl-L-homocysteine
-
 ?
phenylethanolamine + S-adenosyl-L-methionine
-
 Oryctolagus cuniculus N-methylphenylethanolamine + S-adenosyl-L-homocysteine
-
 ?
phenylethanolamine + S-adenosyl-L-methionine 72% of the activity with normetanephrine Macaca mulatta N-methylphenylethanolamine + S-adenosyl-L-homocysteine
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.2 phosphate buffer Macaca mulatta
8 9 Tris buffer Macaca mulatta