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Literature summary for 2.1.1.278 extracted from
- Evolution and function of the Populus SABATH family reveal that a single amino acid change results in a substrate switch (2018), Plant Cell Physiol., 59, 392-403 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene SABATH1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overview, recombinant expression of soluble His-tagged enzyme in Escherichia coli strain BL21(DE3) | Populus trichocarpa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + indole-3-acetate | Populus trichocarpa | - |
methyl indole-3-acetate + S-adenosyl-L-homocysteine | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Populus trichocarpa | B9GJG7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Populus trichocarpa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bud | - |
Populus trichocarpa | - |
| leaf | - |
Populus trichocarpa | - |
| additional information | gene PtSABATH1 is expressed in the root, shoot, leaf and bud tissues, but not expressed in the Populus flower | Populus trichocarpa | - |
| root | - |
Populus trichocarpa | - |
| shoot | - |
Populus trichocarpa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.98 | - |
recombinant wild-type PtSABATH4 with farnesoic acid, pH 7.4, 25°C | Populus trichocarpa |
| 66.51 | - |
recombinant wild-type PtSABATH1 with indole-3-acetate, pH 7.4, 25°C | Populus trichocarpa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | PtSABATH1, also named PtIAMT1, can catalyze the formation of methyl indole-3-acetate (MeIAA) using SAM as a methyl donor and indole-3-acetic acid (IAA) as a methyl acceptor. Enzyme PtSABATH1 does not show any activity towards substrates benzoic acid, jasmonic acid, salicylic acid, vanillic acid, nicotinic acid, coumalic acid, or trans-cinnamic acid. Compared with other Populus SABATH proteins (PtSABATH2, 3, 12, 17, 21 and 24 have very weak activities towards IAA), PtSABATH1 shows at least 40.5fold higher activity towards IAA | Populus trichocarpa | ? | - |
- |
|
| S-adenosyl-L-methionine + farnesoate | low activity | Populus trichocarpa | methyl farnesoate + S-adenosyl-L-homocysteine | - |
? | |
| S-adenosyl-L-methionine + indole-3-acetate | - |
Populus trichocarpa | methyl indole-3-acetate + S-adenosyl-L-homocysteine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| class I SABATH protein | - |
Populus trichocarpa |
| PtIAMT1 | - |
Populus trichocarpa |
| PtSABATH1 | - |
Populus trichocarpa |
| S-adenosyl-L-methionine:salicylic acid carboxyl methyltransferase | UniProt | Populus trichocarpa |
| SABATH methyltransferase 1 | UniProt | Populus trichocarpa |
| SABATH1 | - |
Populus trichocarpa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Populus trichocarpa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Populus trichocarpa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Populus trichocarpa | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the SABATH family, phylogenetic analysis and tree, detailed overview. Twenty-eight Populus SABATH genes are divided into three classes with distinct divergences in their gene structure, expression responses to abiotic stressors and enzymatic properties of encoded proteins. Populus class I SABATH proteins convert indole-3-acetic acid (IAA) to methyl-IAA, class II SABATH proteins convert benzoic acid (BA) and salicylic acid (SA) to methyl-BA and methyl-SA, while class III SABATH proteins convert farnesoic acid (FA) to methyl-FA. Populus class I SABATH gene (PtSABATH1) is grouped into clade I, Populus contains only one class I SABATH gene (PtSABATH1) | Populus trichocarpa |
| metabolism | expression patterns of Populus SABATH genes under normal growth conditions and abiotic stress, overview | Populus trichocarpa |
| physiological function | of Populus SABATH enzymes, only PtSABATH1 has high activity towards indole-3-acetic acid (IAA), and can convert IAA to methyl-IAA, indicating that PtSABATH1 might play an important role in auxin homeostasis | Populus trichocarpa |
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