Data extracted from this reference:
Cloned(Commentary)
expression in Escherichia coli, expression in Arabidopsis thaliana under the control of the 35S cauliflower mosaic virus promoter
Arabidopsis thaliana
Inhibitors
Cu2+
5 mM, more than 90% inhibition
Arabidopsis thaliana
Fe3+
5 mM, more than 90% inhibition
Arabidopsis thaliana
Zn2+
5 mM, more than 90% inhibition
Arabidopsis thaliana
KM Value [mM]
0.0019
gibberellin a9
pH 8.0, 30°C
Arabidopsis thaliana
0.0059
gibberellin a4
pH 8.0, 30°C
Arabidopsis thaliana
Metals/Ions
additional information
not enhanced nor inhibited by K+, NH4+, Na+, Ca2+, Fe2þ, Mg2+, and Mn2+
Arabidopsis thaliana
Molecular Weight [Da]
43300
x * 43300, calculated from sequence
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
S-adenosyl-L-methionine + gibberellin A4
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A4
?
Organism
Arabidopsis thaliana
Q5XF78
Purification (Commentary)
Source Tissue
seed
Arabidopsis thaliana
silique
Arabidopsis thaliana
Substrates and Products (Substrate)
additional information
no activity with gibberelin A12 and gibberelin A19
723404
Arabidopsis thaliana
?
?
S-adenosyl-L-methionine + gibberellin A1
30% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A1
?
S-adenosyl-L-methionine + gibberellin A20
15% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A20
?
S-adenosyl-L-methionine + gibberellin A3
45% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A3
?
S-adenosyl-L-methionine + gibberellin A34
80% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A34
?
S-adenosyl-L-methionine + gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A4
?
S-adenosyl-L-methionine + gibberellin A51
16% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A51
?
S-adenosyl-L-methionine + gibberellin A9
60% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A9
?
Subunits
?
x * 43300, calculated from sequence
Arabidopsis thaliana
Synonyms
GAMT2
Arabidopsis thaliana
gibberellin A4 O-methyltransferase
Arabidopsis thaliana
Temperature Optimum [°C]
30
assay at
Arabidopsis thaliana
Turnover Number [1/s]
0.0015
gibberellin a4
pH 8.0, 30°C
Arabidopsis thaliana
0.0018
gibberellin a9
pH 8.0, 30°C
Arabidopsis thaliana
pH Optimum
Cloned(Commentary) (protein specific)
expression in Escherichia coli, expression in Arabidopsis thaliana under the control of the 35S cauliflower mosaic virus promoter
Arabidopsis thaliana
Inhibitors (protein specific)
Cu2+
5 mM, more than 90% inhibition
Arabidopsis thaliana
Fe3+
5 mM, more than 90% inhibition
Arabidopsis thaliana
Zn2+
5 mM, more than 90% inhibition
Arabidopsis thaliana
KM Value [mM] (protein specific)
0.0019
gibberellin a9
pH 8.0, 30°C
Arabidopsis thaliana
0.0059
gibberellin a4
pH 8.0, 30°C
Arabidopsis thaliana
Metals/Ions (protein specific)
additional information
not enhanced nor inhibited by K+, NH4+, Na+, Ca2+, Fe2þ, Mg2+, and Mn2+
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
43300
x * 43300, calculated from sequence
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
S-adenosyl-L-methionine + gibberellin A4
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A4
?
Purification (Commentary) (protein specific)
Source Tissue (protein specific)
seed
Arabidopsis thaliana
silique
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
additional information
no activity with gibberelin A12 and gibberelin A19
723404
Arabidopsis thaliana
?
?
S-adenosyl-L-methionine + gibberellin A1
30% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A1
?
S-adenosyl-L-methionine + gibberellin A20
15% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A20
?
S-adenosyl-L-methionine + gibberellin A3
45% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A3
?
S-adenosyl-L-methionine + gibberellin A34
80% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A34
?
S-adenosyl-L-methionine + gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A4
?
S-adenosyl-L-methionine + gibberellin A51
16% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A51
?
S-adenosyl-L-methionine + gibberellin A9
60% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A9
?
Subunits (protein specific)
?
x * 43300, calculated from sequence
Arabidopsis thaliana
Temperature Optimum [°C] (protein specific)
30
assay at
Arabidopsis thaliana
Turnover Number [1/s] (protein specific)
0.0015
gibberellin a4
pH 8.0, 30°C
Arabidopsis thaliana
0.0018
gibberellin a9
pH 8.0, 30°C
Arabidopsis thaliana
pH Optimum (protein specific)
Expression
Arabidopsis thaliana
expressed mainly in whole siliques, peak transcript levels from the middle until the end of silique development
up
General Information
malfunction
GAMT2 null mutant shows reduced inhibition of germination, compared with the wild type, when placed on plates containing the gibberellin biosynthesis inhibitor ancymidol
Arabidopsis thaliana
physiological function
methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature
Arabidopsis thaliana
General Information (protein specific)
malfunction
GAMT2 null mutant shows reduced inhibition of germination, compared with the wild type, when placed on plates containing the gibberellin biosynthesis inhibitor ancymidol
Arabidopsis thaliana
physiological function
methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature
Arabidopsis thaliana
Expression (protein specific)
Arabidopsis thaliana
expressed mainly in whole siliques, peak transcript levels from the middle until the end of silique development
up
KCat/KM [mM/s]
0.2
gibberellin a4
pH 8.0, 30°C
Arabidopsis thaliana
0.9
gibberellin a9
pH 8.0, 30°C
Arabidopsis thaliana
KCat/KM [mM/s] (protein specific)
0.2
gibberellin a4
pH 8.0, 30°C
Arabidopsis thaliana
0.9
gibberellin a9
pH 8.0, 30°C
Arabidopsis thaliana
Other publictions for EC 2.1.1.276
723404
Varbanova
Methylation of gibberellins by ...
Arabidopsis thaliana
Plant Cell
19
32-45
2007
-
-
1
-
-
-
3
2
-
1
1
1
-
10
-
-
1
-
-
2
-
-
8
1
2
1
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
2
-
1
1
1
-
-
-
1
-
2
-
-
8
1
1
-
-
2
1
-
-
-
1
2
2
1
2
2
725654
Xing
-
GAMT2 encodes a methyltransfer ...
Arabidopsis thaliana, Arabidopsis thaliana ecotype Columbia-0
J. Integr. Plant Biol.
49
368-381
2007
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
7
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
7
-
-
4
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-