Methylation of gibberellins by Arabidopsis GAMT1 and GAMT2
Varbanova, M.; Yamaguchi, S.; Yang, Y.; McKelvey, K.; Hanada, A.; Borochov, R.; Yu, F.; Jikumaru, Y.; Ross, J.; Cortes, D.; Ma, C.J.; Noel, J.P.; Mander, L.; Shulaev, V.; Kamiya, Y.; Rodermel, S.; Weiss, D.; Pichersky, E.; Plant Cell 19, 32-45 (2007) 
Data extracted from this reference:
Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli, expression in Arabidopsis thaliana under the control of the 35S cauliflower mosaic virus promoter
Arabidopsis thaliana
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
5 mM, more than 90% inhibition
Arabidopsis thaliana
Fe3+
5 mM, more than 90% inhibition
Arabidopsis thaliana
Zn2+
5 mM, more than 90% inhibition
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0019
-
gibberellin a9
pH 8.0, 30°C
Arabidopsis thaliana
0.0059
-
gibberellin a4
pH 8.0, 30°C
Arabidopsis thaliana
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
not enhanced nor inhibited by K+, NH4+, Na+, Ca2+, Fe2þ, Mg2+, and Mn2+
Arabidopsis thaliana
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43300
-
x * 43300, calculated from sequence
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + gibberellin A4
Arabidopsis thaliana
-
S-adenosyl-L-homocysteine + methyl gibberellin A4
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
Q5XF78
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Arabidopsis thaliana
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seed
-
Arabidopsis thaliana
-
silique
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
no activity with gibberelin A12 and gibberelin A19
723404
Arabidopsis thaliana
?
-
-
-
-
S-adenosyl-L-methionine + gibberellin A1
30% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A1
-
-
-
?
S-adenosyl-L-methionine + gibberellin A20
15% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A20
-
-
-
?
S-adenosyl-L-methionine + gibberellin A3
45% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A3
-
-
-
?
S-adenosyl-L-methionine + gibberellin A34
80% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A34
-
-
-
?
S-adenosyl-L-methionine + gibberellin A4
-
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A4
-
-
-
?
S-adenosyl-L-methionine + gibberellin A51
16% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A51
-
-
-
?
S-adenosyl-L-methionine + gibberellin A9
60% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A9
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 43300, calculated from sequence
Arabidopsis thaliana
Synonyms
Synonyms
Commentary
Organism
GAMT2
-
Arabidopsis thaliana
gibberellin A4 O-methyltransferase
-
Arabidopsis thaliana
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Arabidopsis thaliana
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0015
-
gibberellin a4
pH 8.0, 30°C
Arabidopsis thaliana
0.0018
-
gibberellin a9
pH 8.0, 30°C
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli, expression in Arabidopsis thaliana under the control of the 35S cauliflower mosaic virus promoter
Arabidopsis thaliana
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
5 mM, more than 90% inhibition
Arabidopsis thaliana
Fe3+
5 mM, more than 90% inhibition
Arabidopsis thaliana
Zn2+
5 mM, more than 90% inhibition
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0019
-
gibberellin a9
pH 8.0, 30°C
Arabidopsis thaliana
0.0059
-
gibberellin a4
pH 8.0, 30°C
Arabidopsis thaliana
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
not enhanced nor inhibited by K+, NH4+, Na+, Ca2+, Fe2þ, Mg2+, and Mn2+
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43300
-
x * 43300, calculated from sequence
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + gibberellin A4
Arabidopsis thaliana
-
S-adenosyl-L-homocysteine + methyl gibberellin A4
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seed
-
Arabidopsis thaliana
-
silique
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
no activity with gibberelin A12 and gibberelin A19
723404
Arabidopsis thaliana
?
-
-
-
-
S-adenosyl-L-methionine + gibberellin A1
30% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A1
-
-
-
?
S-adenosyl-L-methionine + gibberellin A20
15% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A20
-
-
-
?
S-adenosyl-L-methionine + gibberellin A3
45% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A3
-
-
-
?
S-adenosyl-L-methionine + gibberellin A34
80% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A34
-
-
-
?
S-adenosyl-L-methionine + gibberellin A4
-
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A4
-
-
-
?
S-adenosyl-L-methionine + gibberellin A51
16% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A51
-
-
-
?
S-adenosyl-L-methionine + gibberellin A9
60% of the activity compared to methylation of gibberellin A4
723404
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl gibberellin A9
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 43300, calculated from sequence
Arabidopsis thaliana
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Arabidopsis thaliana
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0015
-
gibberellin a4
pH 8.0, 30°C
Arabidopsis thaliana
0.0018
-
gibberellin a9
pH 8.0, 30°C
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Arabidopsis thaliana
Expression
Organism
Commentary
Expression
Arabidopsis thaliana
expressed mainly in whole siliques, peak transcript levels from the middle until the end of silique development
up
General Information
General Information
Commentary
Organism
malfunction
GAMT2 null mutant shows reduced inhibition of germination, compared with the wild type, when placed on plates containing the gibberellin biosynthesis inhibitor ancymidol
Arabidopsis thaliana
physiological function
methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
malfunction
GAMT2 null mutant shows reduced inhibition of germination, compared with the wild type, when placed on plates containing the gibberellin biosynthesis inhibitor ancymidol
Arabidopsis thaliana
physiological function
methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature
Arabidopsis thaliana
Expression (protein specific)
Organism
Commentary
Expression
Arabidopsis thaliana
expressed mainly in whole siliques, peak transcript levels from the middle until the end of silique development
up
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.2
-
gibberellin a4
pH 8.0, 30°C
Arabidopsis thaliana
0.9
-
gibberellin a9
pH 8.0, 30°C
Arabidopsis thaliana
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.2
-
gibberellin a4
pH 8.0, 30°C
Arabidopsis thaliana
0.9
-
gibberellin a9
pH 8.0, 30°C
Arabidopsis thaliana
Other publictions for EC 2.1.1.276
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723404
Varbanova
Methylation of gibberellins by ...
Arabidopsis thaliana
Plant Cell
19
32-45
2007
-
-
1
-
-
-
3
2
-
1
1
1
-
5
-
-
1
-
-
2
-
-
8
1
2
1
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
2
-
1
1
1
-
-
-
1
-
2
-
-
8
1
1
-
-
2
1
-
-
-
1
2
2
1
2
2
725654
Xing
-
GAMT2 encodes a methyltransfer ...
Arabidopsis thaliana, Arabidopsis thaliana ecotype Columbia-0
J. Integr. Plant Biol.
49
368-381
2007
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
7
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
7
-
-
4
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-