Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | 5 mM, 4fold activation | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, expression in Arabidopsis under the control of the 35S cauliflower mosaic virus promoter | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 5 mM, more than 90% inhibition | Arabidopsis thaliana | |
Fe2+ | 5 mM, more than 90% inhibition | Arabidopsis thaliana | |
Fe3+ | 5 mM, more than 90% inhibition | Arabidopsis thaliana | |
Zn2+ | 5 mM, more than 90% inhibition | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0054 | - |
gibberellin a4 | pH 8.0, 30°C | Arabidopsis thaliana | |
0.0158 | - |
gibberellin a9 | pH 8.0, 30°C | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 5 mM, 3fold activation | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Arabidopsis thaliana |
41500 | - |
x * 41500, calculated from sequence | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + gibberellin A9 | Arabidopsis thaliana | methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature | S-adenosyl-L-homocysteine + methyl gibberellin A9 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | F4JUY5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | GAMT1 expression is localized mostly to seed | Arabidopsis thaliana | - |
silique | expressed mainly in whole siliques, peak transcript levels from the middle until the end of silique development | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + gibberellin 1 | 25% of the activity compared to methylation of gibberellin A9 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A1 | - |
? | |
S-adenosyl-L-methionine + gibberellin A19 | 19% of the activity compared to methylation of gibberellin A9 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A19 | - |
? | |
S-adenosyl-L-methionine + gibberellin A20 | 95% of the activity compared to methylation of gibberellin A9 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A20 | - |
? | |
S-adenosyl-L-methionine + gibberellin A3 | 80% of the activity compared to methylation of gibberellin A9 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A3 | - |
? | |
S-adenosyl-L-methionine + gibberellin A34 | 46% of the activity compared to methylation of gibberellin A9 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A34 | - |
? | |
S-adenosyl-L-methionine + gibberellin A4 | 69% of the activity compared to methylation of gibberellin A9 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A4 | - |
? | |
S-adenosyl-L-methionine + gibberellin A51 | 32% of the activity compared to methylation of gibberellin A9 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A51 | - |
? | |
S-adenosyl-L-methionine + gibberellin A9 | methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A9 | - |
? | |
S-adenosyl-L-methionine + gibberellin A9 | gibberellin A9 i.e. (1R,4aR,4bR,7R,9aR,10S,10aR)-1-methyl-8-methylene-13-oxododecahydro-4a,1-(epoxymethano)-7,9a-methanobenzo[a]azulene-10-carboxylic acid | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A9 | methyl gibberellin A9 i.e. methyl (1R,4aR,4bR,7R,9aR,10S,10aR)-1-methyl-8-methylene-13-oxododecahydro-4a,1-(epoxymethano)-7,9a-methanobenzo[a]azulene-10-carboxylate | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Arabidopsis thaliana |
? | x * 41500, calculated from sequence | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
GAMT1 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
gibberellin a4 | pH 8.0, 30°C | Arabidopsis thaliana | |
0.026 | - |
gibberellin a9 | pH 8.0, 30°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | expressed mainly in whole siliques, peak transcript levels from the middle until the end of silique development | up |
General Information | Comment | Organism |
---|---|---|
malfunction | GAMT1 null mutant shows reduced inhibition of germination, compared with the wild type, when placed on plates containing the gibberellin biosynthesis inhibitor ancymidol | Arabidopsis thaliana |
physiological function | methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
gibberellin a9 | pH 8.0, 30°C | Arabidopsis thaliana | |
1.85 | - |
gibberellin a4 | pH 8.0, 30°C | Arabidopsis thaliana |