Cloned (Comment) | Organism |
---|---|
genes PsSABATH2, DNA and amino acid sequence determination and analysis, sequence comparisons, genetic organization and localization of SABATH genes PsSABATH1-10 in the genome of Picea abies, overview. Sequence similarities among PaSABATHs range from 49% to 91%. Most PaSABATH genes have three introns with the exception of PaSABATH4, PaSABATH5 and PaSABATH10, each of which contains two introns. Phylogenetic analysis and tree | Picea abies |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + benzoate | Picea abies | - |
methyl benzoate + S-adenosyl-L-homocysteine | - |
? | |
S-adenosyl-L-methionine + salicylate | Picea abies | - |
methyl salicylate + S-adenosyl-L-homocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picea abies | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cone | - |
Picea abies | - |
additional information | mixed tissues including needles, stems, and young male cones and female cones are collected from a mature Norway spruce (Picea abies) tree grown on the campus of the University of Tennessee, Knoxville, TN with a GPS coordinates of 35.948 and -83.942 | Picea abies | - |
needle | - |
Picea abies | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | isozyme PaSABATH2 has the highest level of specific activity with salicylic acid and is designated as PaSAMT (EC 2.1.1.274). For comparison, PaSAMT is also assayed with two compounds of similar structure benzoic acid and anthranilic acid (cf. EC 2.1.1.273). While PaSAMT has no activity with anthranilic acid, its activity with benzoic acid is approximately 8% of that with salicylic acid. Radiochemical assay method | Picea abies | ? | - |
- |
|
S-adenosyl-L-methionine + benzoate | - |
Picea abies | methyl benzoate + S-adenosyl-L-homocysteine | - |
? | |
S-adenosyl-L-methionine + benzoate | 8% activity compared to salicylate | Picea abies | methyl benzoate + S-adenosyl-L-homocysteine | - |
? | |
S-adenosyl-L-methionine + salicylate | - |
Picea abies | methyl salicylate + S-adenosyl-L-homocysteine | - |
? | |
S-adenosyl-L-methionine + salicylate | best substrate | Picea abies | methyl salicylate + S-adenosyl-L-homocysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PaSABATH2 | - |
Picea abies |
PaSAMT | - |
Picea abies |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Picea abies |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the protein family of SABATH methyltransferases, ten genes encode isozymes PaSABATH1-10. Five of the PaSABATH isozymes (PaSABATH3, PaSABATH6, PaSABATH7, PaSABATH8, and PaSABATH9) do not show activity with any of the four substrates, i.e. indole-3-acetic acid, jasmonic acid, giberellic acid A3, and salicylic acid, the other five of the PaSABATHs each show activity with one or more of the four substrates. PaSABATH1 has the highest level of specific activity with indole-3-acetic acid and is renamed as PaIAMT (EC 2.1.1.275). PaSABATH2 has the highest level of specific activity with salicylic acid and is designated as PaSAMT (EC 2.1.1.274). For comparison, PaSAMT is also assayed with two compounds of similar structure benzoic acid and anthranilic acid (cf. EC 2.1.1.273). While PaSAMT has no activity with anthranilic acid, its activity with benzoic acid is approximately 8% of that with salicylic acid. PaSABATH4, PaSABATH5 and PaSABATH10 show the highest level of specific activity with jasmonic acid and are renamed PaJAMT1, PaJAMT2, and PaJAMT3, respectively (EC 2.1.1.141). Their products are confirmed to be methyljasmonate | Picea abies |
metabolism | indole-3-acetic acid (IAA), gibberellins (GAs), salicylic acid (SA) and jasmonic acid (JA) exist in methyl ester forms in plants in addition to their free acid forms. The enzymes catalyze methylation of these carboxylic acid phytohormones occurs in form of ten isozymes, PaSABATH1-10 | Picea abies |