BRENDA - Enzyme Database show
show all sequences of 2.1.1.273

Developmental regulation of methyl benzoate biosynthesis and emission in snapdragon flowers

Dudareva, N.; Murfitt, L.M.; Mann, C.J.; Gorenstein, N.; Kolosova, N.; Kish, C.M.; Bonham, C.; Wood, K.; Plant Cell 12, 949-961 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
BAMT cDNA expressed in Escherichia coli
Clarkia breweri
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49000
-
SDS-PAGE
Clarkia breweri
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + benzoate
Clarkia breweri
emission of methyl benzoate occurs in a rhythmic manner, with maximum emission during the day, correlating with pollinator activity
S-adenosyl-L-homocysteine + methyl benzoate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clarkia breweri
-
-
-
Purification (Commentary)
Commentary
Organism
from 5- to 10-day-old upper and lower petal lobes by DE53 anion exchange, phenyl–Sepharose 6FF and MonoQ chromatography
Clarkia breweri
Source Tissue
Source Tissue
Commentary
Organism
Textmining
anther
reduced BAMT activity
Clarkia breweri
-
corolla tube
reduced BAMT activity
Clarkia breweri
-
additional information
no BAMT activity in pistil, stamen, sepal, ovary and leaf tissue
Clarkia breweri
-
petal
in upper and lower lobes, major activity of BAMT
Clarkia breweri
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
emission of methyl benzoate begins at anthesis but at a very low level, 1.8 mg per flower for 24 hr, reaches a peak between days 5 to 8, and declines thereafter, at peak, 56.5 mg is emitted per flower in 24 hr
Clarkia breweri
0.0004518
-
recombinant protein, with benzoic acid as substrate, pH 6.9, 20°C
Clarkia breweri
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no activity detected with salicylic acid and trans-cinnamic acid and their derivatives 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, benzyl alcohol, and 2-,3- and 4-coumaric acid
726156
Clarkia breweri
?
-
-
-
?
S-adenosyl-L-methionine + benzoate
emission of methyl benzoate occurs in a rhythmic manner, with maximum emission during the day, correlating with pollinator activity
726156
Clarkia breweri
S-adenosyl-L-homocysteine + methyl benzoate
-
-
-
?
S-adenosyl-L-methionine + benzoate
methyl benzoate is the most abundant scent compound detected in the majority of snapdragon varieties
726156
Clarkia breweri
S-adenosyl-L-homocysteine + methyl benzoate
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
BAMT cDNA expressed in Escherichia coli
Clarkia breweri
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49000
-
SDS-PAGE
Clarkia breweri
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + benzoate
Clarkia breweri
emission of methyl benzoate occurs in a rhythmic manner, with maximum emission during the day, correlating with pollinator activity
S-adenosyl-L-homocysteine + methyl benzoate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
from 5- to 10-day-old upper and lower petal lobes by DE53 anion exchange, phenyl–Sepharose 6FF and MonoQ chromatography
Clarkia breweri
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
anther
reduced BAMT activity
Clarkia breweri
-
corolla tube
reduced BAMT activity
Clarkia breweri
-
additional information
no BAMT activity in pistil, stamen, sepal, ovary and leaf tissue
Clarkia breweri
-
petal
in upper and lower lobes, major activity of BAMT
Clarkia breweri
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
emission of methyl benzoate begins at anthesis but at a very low level, 1.8 mg per flower for 24 hr, reaches a peak between days 5 to 8, and declines thereafter, at peak, 56.5 mg is emitted per flower in 24 hr
Clarkia breweri
0.0004518
-
recombinant protein, with benzoic acid as substrate, pH 6.9, 20°C
Clarkia breweri
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no activity detected with salicylic acid and trans-cinnamic acid and their derivatives 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, benzyl alcohol, and 2-,3- and 4-coumaric acid
726156
Clarkia breweri
?
-
-
-
?
S-adenosyl-L-methionine + benzoate
emission of methyl benzoate occurs in a rhythmic manner, with maximum emission during the day, correlating with pollinator activity
726156
Clarkia breweri
S-adenosyl-L-homocysteine + methyl benzoate
-
-
-
?
S-adenosyl-L-methionine + benzoate
methyl benzoate is the most abundant scent compound detected in the majority of snapdragon varieties
726156
Clarkia breweri
S-adenosyl-L-homocysteine + methyl benzoate
-
-
-
?
Expression
Organism
Commentary
Expression
Clarkia breweri
during flower life span, levels of methyl benzoate emission, BAMT activity, BAMT gene expression, and the amounts of BAMT protein and benzoic acid are developmentally and differentially regulated
additional information
General Information
General Information
Commentary
Organism
metabolism
final enzyme in the biosynthesis of methyl benzoate
Clarkia breweri
physiological function
attracting pollinators like bumblebees
Clarkia breweri
General Information (protein specific)
General Information
Commentary
Organism
metabolism
final enzyme in the biosynthesis of methyl benzoate
Clarkia breweri
physiological function
attracting pollinators like bumblebees
Clarkia breweri
Expression (protein specific)
Organism
Commentary
Expression
Clarkia breweri
during flower life span, levels of methyl benzoate emission, BAMT activity, BAMT gene expression, and the amounts of BAMT protein and benzoic acid are developmentally and differentially regulated
additional information
Other publictions for EC 2.1.1.273
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736189
Wang
Cloning and characterization o ...
Lilium hybrid cultivar
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14
14510-14521
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1
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1
2
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5
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2
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2
1
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1
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1
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1
1
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1
2
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2
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2
1
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-
-
-
-
-
-
1
-
2
2
-
-
-
736842
Ludwig-Mueller
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Plasmodiophora brassicae
Mol. Plant Pathol.
16
349-364
2015
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1
-
-
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-
-
-
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2
-
5
-
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1
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1
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2
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1
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1
1
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2
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1
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1
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2
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1
1
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-
723460
Köllner
Herbivore-induced SABATH methy ...
Zea mays
Plant Physiol.
153
1795-1807
2010
-
-
1
-
-
-
-
-
-
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-
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-
1
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2
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1
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726114
Effmert
Floral benzenoid carboxyl meth ...
Antirrhinum majus, Arabidopsis lyrata, Arabidopsis thaliana, Nicotiana suaveolens
Phytochemistry
66
1211-1230
2005
-
-
-
-
-
-
-
-
4
-
-
4
-
4
-
-
-
-
-
4
-
-
7
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
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4
-
-
4
-
-
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4
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7
-
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-
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-
-
-
-
-
-
-
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-
723416
Chen
An Arabidopsis thaliana gene f ...
Arabidopsis lyrata, Arabidopsis thaliana
Plant J.
36
577-588
2003
2
-
2
-
-
-
-
4
-
-
8
-
-
6
-
-
2
-
-
6
-
-
8
2
-
-
-
2
2
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
4
-
-
8
-
-
-
-
2
-
6
-
-
8
2
-
-
-
2
2
-
-
-
2
-
-
2
2
2
721415
Negre
Novel S-adenosyl-L-methionine: ...
Antirrhinum majus
Arch. Biochem. Biophys.
406
261-270
2002
-
-
1
-
-
1
6
2
-
-
2
-
-
4
-
-
1
-
-
4
1
1
3
1
-
-
1
2
1
2
-
-
-
-
-
-
-
1
-
-
-
1
-
6
-
2
-
-
2
-
-
-
-
1
-
4
1
1
3
1
-
-
1
2
1
2
-
-
-
-
-
-
-
-
726213
Kolosova
Cellular and subcellular local ...
Antirrhinum majus
Plant Physiol.
126
956-964
2001
-
-
-
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
4
-
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1
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-
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-
-
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1
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1
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-
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4
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
724107
Murfitt
Purification and characterizat ...
Antirrhinum majus
Arch. Biochem. Biophys.
382
145-151
2000
-
-
-
-
-
-
7
4
-
3
3
1
-
4
-
-
1
1
-
2
1
1
2
1
1
-
1
-
1
2
-
-
2
-
-
-
-
-
-
-
-
-
-
7
2
4
-
3
3
1
-
-
-
1
-
2
1
1
2
1
1
-
1
-
1
2
-
-
-
-
-
-
-
-
726156
Dudareva
Developmental regulation of me ...
Clarkia breweri
Plant Cell
12
949-961
2000
-
-
1
-
-
-
-
-
-
-
1
1
-
3
-
-
1
-
-
4
2
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
4
2
-
3
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-