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Literature summary for 2.1.1.271 extracted from

  • Raux, E.; Schubert, H.L.; Woodcock, S.C.; Wilson, K.S.; Warren, M.J.
    Cobalamin (vitamin B12) biosynthesis--cloning, expression and crystallisation of the Bacillus megaterium S-adenosyl-L-methionine-dependent cobalt-precorrin-4 transmethylase CbiF (1998), Eur. J. Biochem., 254, 341-346.
    View publication on PubMed
Search for more literature for 2.1.1.271

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Priestia megaterium
overexpression in Escherichia coli Priestia megaterium

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Priestia megaterium
to 2.4 A resolution. Protein shows two discrete crystal forms, and crystals only grow in the presence of either S-adenosyl-L-methionine or S-adenosyl-L-homocysteine Priestia megaterium

General Stability

General Stability Organism
stable to freezing at -20°C Priestia megaterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 2 * 31000, SDS-PAGE Priestia megaterium
31000
-
 2 * 31000, SDS-PAGE, recombinant protein including His-tag Priestia megaterium
62000
-
 gel filtration Priestia megaterium

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
-
-
Priestia megaterium O87696
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Priestia megaterium
recombinant enzyme Priestia megaterium

Storage Stability

Storage Stability Organism
-20°C, protein reduced in volume to a concentration in excess of 10 mg/ml is stable to freezing and remains in solution when thawed Priestia megaterium
4°C, protein can be stored for several weeks Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + cobalt-precorrin-4
-
 Priestia megaterium S-adenosyl-L-homocysteine + cobalt-precorrin-5
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 31000, SDS-PAGE Priestia megaterium
dimer 2 * 31000, SDS-PAGE, recombinant protein including His-tag Priestia megaterium

Synonyms

Synonyms Comment Organism
CbiF
-
 Priestia megaterium
Cobalt-precorrin-4 C(11)-methyltransferase
-
 Priestia megaterium
cobalt-precorrin-4 transmethylase
-
 Priestia megaterium

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine cofactor, but protein does not associate with S-adenosyl-L-methionine upon gel filtration Priestia megaterium

General Information

General Information Comment Organism
physiological function His-tagged protein is able to complement a Salmonella typhimurium cbiF mutant Priestia megaterium