BRENDA - Enzyme Database show
show all sequences of 2.1.1.270

Stress responses in alfalfa (Medicago sativa L). XXII. cDNA cloning and characterization of an elicitor-inducible isoflavone 7-O-methyltransferase

He, X.Z.; Reddy, J.T.; Dixon, R.A.; Plant Mol. Biol. 36, 43-54 (1998)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Medicago sativa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
x * 41000, SDS-PAGE, recombinant enzyme
Medicago sativa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Medicago sativa
in vivo reaction is 4'-O-methylation of isoflavones, in vitro reaction is 7-O-methylation
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Medicago sativa
O24529
-
-
Purification (Commentary)
Commentary
Organism
-
Medicago sativa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
in vivo reaction is 4'-O-methylation of isoflavones, in vitro reaction is 7-O-methylation
441447
Medicago sativa
?
-
-
-
-
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
at 49% of the activity with daidzein, the enzyme also catalyzes the reaction of EC 2.1.1.150
441447
Medicago sativa
S-adenosyl-L-homocysteine + (+)-pisatin
-
-
-
?
S-adenosyl-L-methionine + (+)-maackiain
at 1.7% of the activity with daidzein, the enzyme also catalyzes the reaction of EC 2.1.1.150
441447
Medicago sativa
S-adenosyl-L-homocysteine + ?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 41000, SDS-PAGE, recombinant enzyme
Medicago sativa
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Medicago sativa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
x * 41000, SDS-PAGE, recombinant enzyme
Medicago sativa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Medicago sativa
in vivo reaction is 4'-O-methylation of isoflavones, in vitro reaction is 7-O-methylation
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Medicago sativa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
in vivo reaction is 4'-O-methylation of isoflavones, in vitro reaction is 7-O-methylation
441447
Medicago sativa
?
-
-
-
-
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
at 49% of the activity with daidzein, the enzyme also catalyzes the reaction of EC 2.1.1.150
441447
Medicago sativa
S-adenosyl-L-homocysteine + (+)-pisatin
-
-
-
?
S-adenosyl-L-methionine + (+)-maackiain
at 1.7% of the activity with daidzein, the enzyme also catalyzes the reaction of EC 2.1.1.150
441447
Medicago sativa
S-adenosyl-L-homocysteine + ?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 41000, SDS-PAGE, recombinant enzyme
Medicago sativa
Expression
Organism
Commentary
Expression
Medicago sativa
IOMT8 transcripts are rapidly induced following elicitation
up
Expression (protein specific)
Organism
Commentary
Expression
Medicago sativa
IOMT8 transcripts are rapidly induced following elicitation
up
Other publictions for EC 2.1.1.270
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
711599
Fondevilla
Identification of genes differ ...
Pisum sativum, Pisum sativum P665
BMC Genomics
12
28
2011
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2
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1
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1
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706124
Kaimoyo
Inactivation of pea genes by R ...
Pisum sativum
Phytochemistry
69
76-87
2008
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1
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1
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1
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3
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1
1
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676377
Akashi
Catalytic specificity of pea O ...
Pisum sativum
Phytochemistry
67
2525-2530
2006
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1
1
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2
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1
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5
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713268
Liu
Blount, J.W.; Huhman, D.; Dixo ...
Medicago truncatula
Plant Cell
18
3656-3669
2006
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1
1
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1
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1
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1
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1
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1
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1
1
441447
He
Stress responses in alfalfa (M ...
Medicago sativa
Plant Mol. Biol.
36
43-54
1998
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