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show all sequences of 2.1.1.269

Tetrahydrofolate serves as a methyl acceptor in the demethylation of dimethylsulfoniopropionate in cell extracts of sulfate-reducing bacteria

Jansen, M.; Hansen, T.A.; Arch. Microbiol. 169, 84-87 (1998)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
O2
preincubation of the extract under air decreases the activity, 35% loss in 3 h
uncultured bacterium
Propyl iodide
0.5 mM, complete inhibition. In the light, the inhibition is far less strong
uncultured bacterium
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
uncultured bacterium
-
-
-
uncultured bacterium WN
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-(S,S-dimethylsulfonio)propanoate + tetrahydrofolate
-
721447
uncultured bacterium
3-(methylthio)propanoate + 5-methyltetrahydrofolate
-
-
-
?
3-(S,S-dimethylsulfonio)propanoate + tetrahydrofolate
-
721447
uncultured bacterium WN
3-(methylthio)propanoate + 5-methyltetrahydrofolate
-
-
-
?
additional information
no substrate: betaine. Reaction does not require ATP or reductive activation by titanium(III)-nitrilotriacetic acid
721447
uncultured bacterium
?
-
-
-
-
additional information
no substrate: betaine. Reaction does not require ATP or reductive activation by titanium(III)-nitrilotriacetic acid
721447
uncultured bacterium WN
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
28
-
assay at
uncultured bacterium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
uncultured bacterium
Cofactor
Cofactor
Commentary
Organism
Structure
tetrahydrofolate
methyl acceptor in the anaerobic demethylation of dimethylsulfoniopropionate to methylthiopropionate
uncultured bacterium
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
tetrahydrofolate
methyl acceptor in the anaerobic demethylation of dimethylsulfoniopropionate to methylthiopropionate
uncultured bacterium
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
O2
preincubation of the extract under air decreases the activity, 35% loss in 3 h
uncultured bacterium
Propyl iodide
0.5 mM, complete inhibition. In the light, the inhibition is far less strong
uncultured bacterium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-(S,S-dimethylsulfonio)propanoate + tetrahydrofolate
-
721447
uncultured bacterium
3-(methylthio)propanoate + 5-methyltetrahydrofolate
-
-
-
?
3-(S,S-dimethylsulfonio)propanoate + tetrahydrofolate
-
721447
uncultured bacterium WN
3-(methylthio)propanoate + 5-methyltetrahydrofolate
-
-
-
?
additional information
no substrate: betaine. Reaction does not require ATP or reductive activation by titanium(III)-nitrilotriacetic acid
721447
uncultured bacterium
?
-
-
-
-
additional information
no substrate: betaine. Reaction does not require ATP or reductive activation by titanium(III)-nitrilotriacetic acid
721447
uncultured bacterium WN
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
28
-
assay at
uncultured bacterium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
uncultured bacterium
Other publictions for EC 2.1.1.269
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Cui
Abundance and distribution of ...
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Appl. Environ. Microbiol.
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9
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3
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-
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-
-
-
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-
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6
6
-
-
-
736286
Varaljay
Single-taxon field measurement ...
Roseobacter sp., Roseobacter sp. HTCC 2255, Ruegeria pomeroyi
ISME J.
9
1677-1686
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2
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-
-
-
-
-
-
-
-
3
-
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-
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-
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2
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-
-
-
-
-
-
-
-
-
-
-
-
-
4
4
-
-
-
720998
Schuller
Structures of dimethylsulfonio ...
Candidatus Pelagibacter ubique, Candidatus Pelagibacter ubique HTCC1062
Protein Sci.
21
289-298
2012
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-
1
1
-
-
-
-
-
-
-
1
-
6
-
-
1
1
-
-
-
-
2
1
-
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-
-
-
-
1
-
-
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-
1
1
2
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
718639
Howard
Changes in dimethylsulfoniopro ...
Candidatus Pelagibacter ubique, Candidatus Pelagibacter ubique HTCC7211, Candidatus Puniceispirillum marinum, Flavobacteriaceae, Rhodobacterales, Rhodospirillales, Roseobacter sp., Sphingomonadales, unidentified marine bacterioplankton, unidentified marine bacterioplankton HTCC2080
Appl. Environ. Microbiol.
77
524-531
2011
-
-
4
-
-
-
-
-
-
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
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-
-
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-
-
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4
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
8
-
-
-
698600
Reisch
Dimethylsulfoniopropionate-dep ...
Candidatus Pelagibacter ubique, Candidatus Pelagibacter ubique HTCC1062, Ruegeria pomeroyi
J. Bacteriol.
190
8018-8024
2008
-
-
2
-
-
-
8
7
-
-
4
-
-
12
-
-
2
-
-
-
-
-
12
2
-
-
-
4
2
2
-
-
2
-
-
-
-
2
-
-
-
-
-
8
2
7
-
-
5
-
-
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-
3
-
-
-
-
12
3
-
-
-
4
3
3
-
-
-
-
-
-
2
2
701221
Howard
Bacterial taxa that limit sulf ...
Candidatus Pelagibacter ubique, Ruegeria pomeroyi DSS-3
Science
314
649-652
2006
-
-
1
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2
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4
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4
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2
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1
2
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-
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2
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4
-
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-
-
-
-
-
-
-
-
-
-
-
-
721447
Jansen
Tetrahydrofolate serves as a m ...
uncultured bacterium, uncultured bacterium WN
Arch. Microbiol.
169
84-87
1998
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
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4
-
1
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-
1
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