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Literature summary for 2.1.1.267 extracted from

  • Lee, D.; Park, H.L.; Lee, S.W.; Bhoo, S.H.; Cho, M.H.
    Biotechnological production of dimethoxyflavonoids using a fusion flavonoid O-methyltransferase possessing both 3- and 7-O-methyltransferase activities (2017), J. Nat. Prod., 80, 1467-1474 .
    View publication on PubMed
Search for more literature for 2.1.1.267

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type anchimeric mutant enzymes in Escherichia coli Solanum lycopersicum

Protein Variants

Protein Variants Comment Organism
additional information construction of a multifunctional flavonoid O-methyltransferase fusing tomato 3'-O-methyltransferase OMT3 and Oryza sativa naringenin 7-O-methyltransferase NOMT. The OMT3/NOMT fusion enzyme possesses both 3'- and 7-OMT activities to diverse flavonoid substrates, which are comparable to those of individual OMT3 and NOMT. The OMT3/OsNOMT enzyme also shows 3'- and 7-OMT activity for 7- or 3'-O-methylflavonoids, respectively. The biotransformation of the flavonoids quercetin, luteolin, eriodictyol, and taxifolin using OMT3/NOMT-transformed Escherichia coli generates corresponding di-O-methylflavonoids, rhamnazin, velutin, 3',7-di-O-methyleriodictyol, and 3',7-di-Omethyltaxifolin, respectively Solanum lycopersicum
additional information generation of a multifunctional FOMT fusing a 3'-OMT (SlOMT3, EC 2.1.1.267) and a 7-OMT (OsNOMT, EC 2.1.1.82). The SlOMT3/OsNOMT fusion enzyme possesses both 3'- and 7-OMT activities to diverse flavonoid substrates, which are comparable to those of individual SlOMT3 and OsNOMT. The SlOMT3/OsNOMT enzyme also shows 3'- and 7-OMT activity for 7- or 3'-O-methylflavonoids, respectively, suggesting that the fusion enzyme can sequentially methylate flavonoids into di-O-methylflavonoids. The biotransformation of the flavonoids quercetin, luteolin, eriodictyol, and taxifolin using SlOMT3/OsNOMT-transformed Escherichia coli generated corresponding di-O-methylflavonoids, rhamnazin, velutin, 3',7-di-O-methyleriodictyol, and 3',7-di-O-methyltaxifolin, respectively. These results indicate that dimethoxyflavonoids may be efficiently produced from non-methylated flavonoid precursors through a one-step biotransformation using the engineered Escherichia coli harboring the SlOMT3/OsNOMT fusion gene Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + a 3'-hydroxyflavonoid Solanum lycopersicum
-
 S-adenosyl-L-homocysteine + a 3'-methoxyflavonoid
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-
Solanum lycopersicum K4D2J5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + a 3'-hydroxyflavonoid
-
 Solanum lycopersicum S-adenosyl-L-homocysteine + a 3'-methoxyflavonoid
-
 ?

Synonyms

Synonyms Comment Organism
3'-OMT
-
 Solanum lycopersicum
flavonoid O-methyltransferase
-
 Solanum lycopersicum
SlOMT3
-
 Solanum lycopersicum

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Solanum lycopersicum