BRENDA - Enzyme Database show
show all sequences of 2.1.1.267

An O-methyltransferase modifies accumulation of methylated anthocyanins in seedlings of tomato

Gomez Roldan, M.V.; Outchkourov, N.; van Houwelingen, A.; Lammers, M.; Romero de la Fuente, I.; Ziklo, N.; Aharoni, A.; Hall, R.D.; Beekwilder, J.; Plant J. 80, 695-708 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene OMT, DNA and amino acid sequence determination and analysis, phylogenetic analysis, gene OMT shows patterns strongly correlating with both accumulation of anthocyanins and expression of anthocyanin biosynthesis genes, encoding dihydroflavonol reductase (DFR), flavonoid-3',5'-hydroxylase (F3'5'H), and anthocyanidin synthase (ANS)
Solanum lycopersicum
Engineering
Amino acid exchange
Commentary
Organism
additional information
silencing of AnthOMT in hypocotyl tissue, several hypocotyls of three lines (57, 85 and 87) show an anthocyanin profile that is different to that of the wild-type, and show a strong reduction in malvidin-type anthocyanins, while other lines (64, 66 and 71) show only wild-type anthocyanin profiles
Solanum lycopersicum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.012
-
delphinidin 3-O-beta-D-glucoside
pH 7.0, 30°C
Solanum lycopersicum
0.1
-
quercetin
pH 7.0, 30°C
Solanum lycopersicum
0.437
-
quercetin 3-O-beta-D-glucoside
pH 7.0, 30°C
Solanum lycopersicum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26300
-
x * 26300, SDS-PAGE
Solanum lycopersicum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Solanum lycopersicum
the enzyme AnthOMT shows a strong affinity for glycosylated anthocyanins, while other flavonoid glycosides and aglycones are much less preferred
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Solanum lycopersicum
-
var. MoneyMaker, gene OMT
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cotyledon
-
Solanum lycopersicum
-
fruit
-
Solanum lycopersicum
-
hypocotyl
-
Solanum lycopersicum
-
additional information
the OMT gene shows patterns strongly correlating with both accumulation of anthocyanins and expression of anthocyanin biosynthesis genes
Solanum lycopersicum
-
root
-
Solanum lycopersicum
-
seedling
-
Solanum lycopersicum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme AnthOMT shows a strong affinity for glycosylated anthocyanins, while other flavonoid glycosides and aglycones are much less preferred
736999
Solanum lycopersicum
?
-
-
-
-
additional information
the specificity of AnthOMT is mainly directed towards the 3' and 5' positions of the anthocyanin B-ring, to produce petunidin and malvidin glucosides, and is not active at the 4' position, substrate specificity of enzyme AnthOMT, overview. Poor activity with caffeic acid, no activity with pelargonidin-3-glucoside and delphinidin. AnthOMT is able to methylate the more complex substrates, e.g. an an extract of semi-polar compounds from Ros/Del tomato fruits preparation, resulting in a strong increase in malvidin 3-(4-coumaroyl)-rutinoside-5-glucoside
736999
Solanum lycopersicum
?
-
-
-
-
S-adenosyl-L-methionine + cyanidin 3-O-beta-D-glucoside
-
736999
Solanum lycopersicum
S-adenosyl-L-homocysteine + peonidin 3-O-beta-D-glucoside
-
-
-
?
S-adenosyl-L-methionine + delphinidin 3-O-beta-D-glucoside
high activity
736999
Solanum lycopersicum
S-adenosyl-L-homocysteine + petunidin 3-O-beta-D-glucoside
-
-
-
?
S-adenosyl-L-methionine + myricitin
-
736999
Solanum lycopersicum
S-adenosyl-L-homocysteine + laricitrin
two product isomers
-
-
?
S-adenosyl-L-methionine + quercetin
-
736999
Solanum lycopersicum
S-adenosyl-L-homocysteine + isorhamnetin
-
-
-
?
S-adenosyl-L-methionine + quercetin 3-O-beta-D-glucoside
-
736999
Solanum lycopersicum
S-adenosyl-L-homocysteine + isorhamnetin 3-O-beta-D-glucoside
-
-
-
?
S-adenosyl-L-methionine + quercetin 3-O-beta-D-rutinoside
-
736999
Solanum lycopersicum
S-adenosyl-L-homocysteine + isorhamnetin 3-O-beta-D-rutinoside
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 26300, SDS-PAGE
Solanum lycopersicum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
40
-
Solanum lycopersicum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.014
-
quercetin 3-O-beta-D-glucoside
pH 7.0, 30°C
Solanum lycopersicum
0.02
-
quercetin
pH 7.0, 30°C
Solanum lycopersicum
0.025
-
delphinidin 3-O-beta-D-glucoside
pH 7.0, 30°C
Solanum lycopersicum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
9
-
Solanum lycopersicum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
9
loss of activity at pH 5.0-6.0, inactive below
Solanum lycopersicum
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Solanum lycopersicum
Cloned(Commentary) (protein specific)
Commentary
Organism
gene OMT, DNA and amino acid sequence determination and analysis, phylogenetic analysis, gene OMT shows patterns strongly correlating with both accumulation of anthocyanins and expression of anthocyanin biosynthesis genes, encoding dihydroflavonol reductase (DFR), flavonoid-3',5'-hydroxylase (F3'5'H), and anthocyanidin synthase (ANS)
Solanum lycopersicum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Solanum lycopersicum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
silencing of AnthOMT in hypocotyl tissue, several hypocotyls of three lines (57, 85 and 87) show an anthocyanin profile that is different to that of the wild-type, and show a strong reduction in malvidin-type anthocyanins, while other lines (64, 66 and 71) show only wild-type anthocyanin profiles
Solanum lycopersicum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.012
-
delphinidin 3-O-beta-D-glucoside
pH 7.0, 30°C
Solanum lycopersicum
0.1
-
quercetin
pH 7.0, 30°C
Solanum lycopersicum
0.437
-
quercetin 3-O-beta-D-glucoside
pH 7.0, 30°C
Solanum lycopersicum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26300
-
x * 26300, SDS-PAGE
Solanum lycopersicum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Solanum lycopersicum
the enzyme AnthOMT shows a strong affinity for glycosylated anthocyanins, while other flavonoid glycosides and aglycones are much less preferred
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cotyledon
-
Solanum lycopersicum
-
fruit
-
Solanum lycopersicum
-
hypocotyl
-
Solanum lycopersicum
-
additional information
the OMT gene shows patterns strongly correlating with both accumulation of anthocyanins and expression of anthocyanin biosynthesis genes
Solanum lycopersicum
-
root
-
Solanum lycopersicum
-
seedling
-
Solanum lycopersicum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme AnthOMT shows a strong affinity for glycosylated anthocyanins, while other flavonoid glycosides and aglycones are much less preferred
736999
Solanum lycopersicum
?
-
-
-
-
additional information
the specificity of AnthOMT is mainly directed towards the 3' and 5' positions of the anthocyanin B-ring, to produce petunidin and malvidin glucosides, and is not active at the 4' position, substrate specificity of enzyme AnthOMT, overview. Poor activity with caffeic acid, no activity with pelargonidin-3-glucoside and delphinidin. AnthOMT is able to methylate the more complex substrates, e.g. an an extract of semi-polar compounds from Ros/Del tomato fruits preparation, resulting in a strong increase in malvidin 3-(4-coumaroyl)-rutinoside-5-glucoside
736999
Solanum lycopersicum
?
-
-
-
-
S-adenosyl-L-methionine + cyanidin 3-O-beta-D-glucoside
-
736999
Solanum lycopersicum
S-adenosyl-L-homocysteine + peonidin 3-O-beta-D-glucoside
-
-
-
?
S-adenosyl-L-methionine + delphinidin 3-O-beta-D-glucoside
high activity
736999
Solanum lycopersicum
S-adenosyl-L-homocysteine + petunidin 3-O-beta-D-glucoside
-
-
-
?
S-adenosyl-L-methionine + myricitin
-
736999
Solanum lycopersicum
S-adenosyl-L-homocysteine + laricitrin
two product isomers
-
-
?
S-adenosyl-L-methionine + quercetin
-
736999
Solanum lycopersicum
S-adenosyl-L-homocysteine + isorhamnetin
-
-
-
?
S-adenosyl-L-methionine + quercetin 3-O-beta-D-glucoside
-
736999
Solanum lycopersicum
S-adenosyl-L-homocysteine + isorhamnetin 3-O-beta-D-glucoside
-
-
-
?
S-adenosyl-L-methionine + quercetin 3-O-beta-D-rutinoside
-
736999
Solanum lycopersicum
S-adenosyl-L-homocysteine + isorhamnetin 3-O-beta-D-rutinoside
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 26300, SDS-PAGE
Solanum lycopersicum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
40
-
Solanum lycopersicum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.014
-
quercetin 3-O-beta-D-glucoside
pH 7.0, 30°C
Solanum lycopersicum
0.02
-
quercetin
pH 7.0, 30°C
Solanum lycopersicum
0.025
-
delphinidin 3-O-beta-D-glucoside
pH 7.0, 30°C
Solanum lycopersicum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
9
-
Solanum lycopersicum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
9
loss of activity at pH 5.0-6.0, inactive below
Solanum lycopersicum
General Information
General Information
Commentary
Organism
malfunction
downregulation of AnthOMT in planta decreases the representation of methylated anthocyanins in tomato tissues as well as overall anthocyanin accumulation
Solanum lycopersicum
physiological function
role of the enzyme in the diversification of tomato anthocyanins
Solanum lycopersicum
General Information (protein specific)
General Information
Commentary
Organism
malfunction
downregulation of AnthOMT in planta decreases the representation of methylated anthocyanins in tomato tissues as well as overall anthocyanin accumulation
Solanum lycopersicum
physiological function
role of the enzyme in the diversification of tomato anthocyanins
Solanum lycopersicum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.032
-
quercetin 3-O-beta-D-glucoside
pH 7.0, 30°C
Solanum lycopersicum
0.2
-
quercetin
pH 7.0, 30°C
Solanum lycopersicum
2.08
-
delphinidin 3-O-beta-D-glucoside
pH 7.0, 30°C
Solanum lycopersicum
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.032
-
quercetin 3-O-beta-D-glucoside
pH 7.0, 30°C
Solanum lycopersicum
0.2
-
quercetin
pH 7.0, 30°C
Solanum lycopersicum
2.08
-
delphinidin 3-O-beta-D-glucoside
pH 7.0, 30°C
Solanum lycopersicum
Other publictions for EC 2.1.1.267
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737288
Azuma
-
MYB diplotypes at the color lo ...
Vitis vinifera
Tree Genet. Genomes
11
0000
2015
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736999
Gomez Roldan
An O-methyltransferase modifie ...
Solanum lycopersicum
Plant J.
80
695-708
2014
-
-
1
-
1
-
-
3
-
-
1
1
-
3
-
-
-
-
-
6
-
-
8
1
1
-
-
3
1
1
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
3
-
-
1
1
-
-
-
-
-
6
-
-
8
1
1
-
-
3
1
1
-
-
-
2
2
-
3
3
734429
Cho
-
Characterization of regiospeci ...
Solanum lycopersicum
J. Korean Soc. Appl. Biol. Chem.
55
749-755
2012
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
1
-
-
5
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
3
-
-
-
-
-
-
-
-
3
3
721944
Fournier-Level
Genetic mechanisms underlying ...
Vitis vinifera
BMC Plant Biol.
11
179
2011
-
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
4
4
-
-
-
720730
Hugueney
A novel cation-dependent O-met ...
Vitis vinifera
Plant Physiol.
150
2057-2070
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697062
Jeong
-
Flavonoids as substrates of Ba ...
Bacillus halodurans
Bull. Korean Chem. Soc.
29
1311-1314
2008
-
-
1
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700855
Lee
Cation dependent O-methyltrans ...
Oryza sativa
Planta
227
641-647
2008
-
-
1
-
8
-
-
8
-
5
4
-
-
2
-
-
-
-
-
3
-
-
15
1
1
-
-
-
1
-
-
1
-
-
-
-
-
2
2
-
12
-
-
-
-
9
-
8
4
-
-
-
-
-
-
5
-
-
15
2
2
-
-
-
2
-
-
-
-
-
-
-
-
-
637316
Cacace
A flavonol O-methyltransferase ...
Catharanthus roseus
Phytochemistry
62
127-137
2003
-
-
1
-
-
-
-
-
-
-
2
3
-
5
-
-
1
-
-
1
1
-
17
1
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
3
-
-
-
2
-
2
1
-
17
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-