BRENDA - Enzyme Database show
show all sequences of 2.1.1.267

A flavonol O-methyltransferase from Catharanthus roseus performing two sequential methylations

Cacace, S.; Schröder, G.; Wehinger, E.; Strack, D.; Schmidt, J.; Schröder, J.; Phytochemistry 62, 127-137 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
; gene CrOMT2, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, expression as His-tagged nezyme in Escherichia coli
Catharanthus roseus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
SDS-PAGE
Catharanthus roseus
39145
-
x * 39145, sequence calculation
Catharanthus roseus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Catharanthus roseus
CROMT2 is involved in biosynthesis of glycosides and anthocyanins
?
-
-
-
additional information
Catharanthus roseus
OMT2 performs two sequential methylations at the 3'-and 5'-positions of the B-ring in myricetin (flavonol) and dihydromyricetin (dihydroflavonol)
?
-
-
-
S-adenosyl-L-methionine + myricetin
Catharanthus roseus
involvement in biosynthesis of flavonol glycosides and anthocyanins
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Catharanthus roseus
-
; Madagascar periwinkle
-
Catharanthus roseus
Q8GSN1
gene CrOMT2
-
Purification (Commentary)
Commentary
Organism
; recombinant His-tagged OMT2 by nickel affinity chromatography
Catharanthus roseus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
; dark-grown
Catharanthus roseus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000219
-
-
Catharanthus roseus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
CROMT2 is involved in biosynthesis of glycosides and anthocyanins
637316
Catharanthus roseus
?
-
-
-
-
additional information
OMT2 performs two sequential methylations at the 3'-and 5'-positions of the B-ring in myricetin (flavonol) and dihydromyricetin (dihydroflavonol)
637316
Catharanthus roseus
?
-
-
-
-
additional information
no activity with kaempferol, dihydrokaempferol, caffeate and caffeate esters
637316
Catharanthus roseus
?
-
-
-
-
S-adenosyl-L-methionine + 2,3-dihydromyricetin
-
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + 3'-O-methyl-2,3-dihydromyricetin
-
-
-
?
S-adenosyl-L-methionine + 3'-O-methyl-2,3-dihydromyricetin
-
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + 3',5'-di-O-methyl-2,3-dihydromyricetin
i.e. syringetin
-
-
?
S-adenosyl-L-methionine + 3'-O-methyl-2,3-dihydroquercetin
-
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + 3',4'-di-O-methyl-2,3-dihydroquercetin
-
-
-
?
S-adenosyl-L-methionine + 3'-O-methylmyricetin
-
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + 3',5'-di-O-methylmyricetin
-
-
-
?
S-adenosyl-L-methionine + 3'-O-methylquercetin
-
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + 3',4'-di-O-methylquercetin
-
-
-
?
S-adenosyl-L-methionine + dihydromyricetin
two sequential methylations at the 3'- and 5'-positions of the B-ring in dihydromyricetin
637316
Catharanthus roseus
?
-
-
-
?
S-adenosyl-L-methionine + dihydromyricetin
54% of the activity with myricetin
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + dihydroquercetin
22% of the activity with myricetin
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + dihydroquercetin
-
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + 3'-O-methyl-2,3-dihydroquercetin
-
-
-
?
S-adenosyl-L-methionine + myricetin
two sequential methylations at the 3'- and 5'-positions of the B-ring in myricetin, activity is strictly confined to flavonols and dihydroflavonols, it requires at least 2 B-ring hydroxyl groups
637316
Catharanthus roseus
?
-
-
-
?
S-adenosyl-L-methionine + myricetin
involvement in biosynthesis of flavonol glycosides and anthocyanins
637316
Catharanthus roseus
?
-
-
-
-
S-adenosyl-L-methionine + myricetin
-
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + syringetin
-
-
-
?
S-adenosyl-L-methionine + myricetin
best substrate
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + 3'-O-methylmyricetin
-
-
-
?
S-adenosyl-L-methionine + quercetin
low activity
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + 3'-O-methylquercetin
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 39145, sequence calculation
Catharanthus roseus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Catharanthus roseus
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Catharanthus roseus
gene CrOMT2, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, expression as His-tagged nezyme in Escherichia coli
Catharanthus roseus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
SDS-PAGE
Catharanthus roseus
39145
-
x * 39145, sequence calculation
Catharanthus roseus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Catharanthus roseus
CROMT2 is involved in biosynthesis of glycosides and anthocyanins
?
-
-
-
additional information
Catharanthus roseus
OMT2 performs two sequential methylations at the 3'-and 5'-positions of the B-ring in myricetin (flavonol) and dihydromyricetin (dihydroflavonol)
?
-
-
-
S-adenosyl-L-methionine + myricetin
Catharanthus roseus
involvement in biosynthesis of flavonol glycosides and anthocyanins
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Catharanthus roseus
recombinant His-tagged OMT2 by nickel affinity chromatography
Catharanthus roseus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
dark-grown
Catharanthus roseus
-
cell suspension culture
-
Catharanthus roseus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000219
-
-
Catharanthus roseus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
CROMT2 is involved in biosynthesis of glycosides and anthocyanins
637316
Catharanthus roseus
?
-
-
-
-
additional information
OMT2 performs two sequential methylations at the 3'-and 5'-positions of the B-ring in myricetin (flavonol) and dihydromyricetin (dihydroflavonol)
637316
Catharanthus roseus
?
-
-
-
-
additional information
no activity with kaempferol, dihydrokaempferol, caffeate and caffeate esters
637316
Catharanthus roseus
?
-
-
-
-
S-adenosyl-L-methionine + 2,3-dihydromyricetin
-
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + 3'-O-methyl-2,3-dihydromyricetin
-
-
-
?
S-adenosyl-L-methionine + 3'-O-methyl-2,3-dihydromyricetin
-
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + 3',5'-di-O-methyl-2,3-dihydromyricetin
i.e. syringetin
-
-
?
S-adenosyl-L-methionine + 3'-O-methyl-2,3-dihydroquercetin
-
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + 3',4'-di-O-methyl-2,3-dihydroquercetin
-
-
-
?
S-adenosyl-L-methionine + 3'-O-methylmyricetin
-
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + 3',5'-di-O-methylmyricetin
-
-
-
?
S-adenosyl-L-methionine + 3'-O-methylquercetin
-
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + 3',4'-di-O-methylquercetin
-
-
-
?
S-adenosyl-L-methionine + dihydromyricetin
two sequential methylations at the 3'- and 5'-positions of the B-ring in dihydromyricetin
637316
Catharanthus roseus
?
-
-
-
?
S-adenosyl-L-methionine + dihydromyricetin
54% of the activity with myricetin
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + dihydroquercetin
22% of the activity with myricetin
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + dihydroquercetin
-
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + 3'-O-methyl-2,3-dihydroquercetin
-
-
-
?
S-adenosyl-L-methionine + myricetin
two sequential methylations at the 3'- and 5'-positions of the B-ring in myricetin, activity is strictly confined to flavonols and dihydroflavonols, it requires at least 2 B-ring hydroxyl groups
637316
Catharanthus roseus
?
-
-
-
?
S-adenosyl-L-methionine + myricetin
involvement in biosynthesis of flavonol glycosides and anthocyanins
637316
Catharanthus roseus
?
-
-
-
-
S-adenosyl-L-methionine + myricetin
-
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + syringetin
-
-
-
?
S-adenosyl-L-methionine + myricetin
best substrate
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + 3'-O-methylmyricetin
-
-
-
?
S-adenosyl-L-methionine + quercetin
low activity
637316
Catharanthus roseus
S-adenosyl-L-homocysteine + 3'-O-methylquercetin
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 39145, sequence calculation
Catharanthus roseus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Catharanthus roseus
General Information
General Information
Commentary
Organism
metabolism
CrOMT2 is involved in the biosynthesis of flavonol glycosides and anthocyanins in Catharanthus roeus
Catharanthus roseus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
CrOMT2 is involved in the biosynthesis of flavonol glycosides and anthocyanins in Catharanthus roeus
Catharanthus roseus
Other publictions for EC 2.1.1.267
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737288
Azuma
-
MYB diplotypes at the color lo ...
Vitis vinifera
Tree Genet. Genomes
11
0000
2015
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736999
Gomez Roldan
An O-methyltransferase modifie ...
Solanum lycopersicum
Plant J.
80
695-708
2014
-
-
1
-
1
-
-
3
-
-
1
1
-
3
-
-
-
-
-
6
-
-
8
1
1
-
-
3
1
1
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
3
-
-
1
1
-
-
-
-
-
6
-
-
8
1
1
-
-
3
1
1
-
-
-
2
2
-
3
3
734429
Cho
-
Characterization of regiospeci ...
Solanum lycopersicum
J. Korean Soc. Appl. Biol. Chem.
55
749-755
2012
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
1
-
-
5
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
3
-
-
-
-
-
-
-
-
3
3
721944
Fournier-Level
Genetic mechanisms underlying ...
Vitis vinifera
BMC Plant Biol.
11
179
2011
-
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
4
4
-
-
-
720730
Hugueney
A novel cation-dependent O-met ...
Vitis vinifera
Plant Physiol.
150
2057-2070
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697062
Jeong
-
Flavonoids as substrates of Ba ...
Bacillus halodurans
Bull. Korean Chem. Soc.
29
1311-1314
2008
-
-
1
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700855
Lee
Cation dependent O-methyltrans ...
Oryza sativa
Planta
227
641-647
2008
-
-
1
-
8
-
-
8
-
5
4
-
-
2
-
-
-
-
-
3
-
-
15
1
1
-
-
-
1
-
-
1
-
-
-
-
-
2
2
-
12
-
-
-
-
9
-
8
4
-
-
-
-
-
-
5
-
-
15
2
2
-
-
-
2
-
-
-
-
-
-
-
-
-
637316
Cacace
A flavonol O-methyltransferase ...
Catharanthus roseus
Phytochemistry
62
127-137
2003
-
-
1
-
-
-
-
-
-
-
2
3
-
5
-
-
1
-
-
1
1
-
17
1
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
3
-
-
-
2
-
2
1
-
17
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-