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show all sequences of 2.1.1.266

Purification, crystallization and preliminary X-ray diffraction analysis of the 23S rRNA methyltransferase RlmJ from Escherichia coli

Punekar, A.S.; Selmer, M.; Acta Crystallogr. Sect. F 69, 1001-1003 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene yhiR, recombinant expresssion of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Escherichia coli
Crystallization (Commentary)
Crystallization
Organism
purified recombinant C-terminally His6-tagged enzyme, sitting drop vapour diffusion method, mixing of 0.0015 ml of protein solution with an equal amount of reservoir solution containing 0.2 M sodium sulfate decahydrate, 0.1 M Tris-HCl pH 8.5, 30% w/v PEG 4000, and equilibration against 0.08 ml of reservoir solution, 8 days, 20°C, X-ray diffraction strucure determination and analysis at 1.8 A resolution
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + adenine2030 in 23S rRNA
Escherichia coli
-
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P37634
MG1655, gene yhiR
-
Purification (Commentary)
Commentary
Organism
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + adenine2030 in 23S rRNA
-
735378
Escherichia coli
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + adenine2030 in 23S rRNA
enzyme RlmJ uses the cofactor S-adenosyl-L-methionine to methylate the exocyclic nitrogen N6 of nucleotide A2030 in 23S rRNA during ribosome assembly in Escherichia coli
735378
Escherichia coli
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
dependent on
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
gene yhiR, recombinant expresssion of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
dependent on
Escherichia coli
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified recombinant C-terminally His6-tagged enzyme, sitting drop vapour diffusion method, mixing of 0.0015 ml of protein solution with an equal amount of reservoir solution containing 0.2 M sodium sulfate decahydrate, 0.1 M Tris-HCl pH 8.5, 30% w/v PEG 4000, and equilibration against 0.08 ml of reservoir solution, 8 days, 20°C, X-ray diffraction strucure determination and analysis at 1.8 A resolution
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + adenine2030 in 23S rRNA
Escherichia coli
-
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + adenine2030 in 23S rRNA
-
735378
Escherichia coli
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + adenine2030 in 23S rRNA
enzyme RlmJ uses the cofactor S-adenosyl-L-methionine to methylate the exocyclic nitrogen N6 of nucleotide A2030 in 23S rRNA during ribosome assembly in Escherichia coli
735378
Escherichia coli
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA
-
-
-
?
Other publictions for EC 2.1.1.266
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735378
Punekar
Purification, crystallization ...
Escherichia coli
Acta Crystallogr. Sect. F
69
1001-1003
2013
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736901
Punekar
Structural and functional insi ...
Escherichia coli
Nucleic Acids Res.
41
9537-9548
2013
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1
1
6
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4
4
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721036
Golovina
The last rRNA methyltransferas ...
Escherichia coli
RNA
18
1725-1734
2012
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