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Literature summary for 2.1.1.265 extracted from
- Escherichia coli TehB requires S-adenosylmethionine as a cofactor to mediate tellurite resistance (2000), J. Bacteriol., 182, 6509-6513.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene tehB, expression of His6-tagged wild-type enzyme in Escherichia coli strain coli C41(DE3), expression of the mutant TehB enzymes in tehAB operon from pTWT101, a pTZ19R clone, in Escherichia coli strain JM109, expression of His6-tagged mutant enzymes in Escherichia coli strain BL21(DE3) | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D36A | site-directed mutagenesis of a motif I residue, the tellurite resistance of the mutant is abolished | Escherichia coli |
| D36N | site-directed mutagenesis of a motif I residue, the tellurite resistance of the mutant is abolished | Escherichia coli |
| D59A | site-directed mutagenesis of a motif I residue, the tellurite resistance of the mutant is abolished | Escherichia coli |
| D97E | site-directed mutagenesis of a motif II residue, the tellurite resistance of the mutant is unaltered | Escherichia coli |
| D97N | site-directed mutagenesis of a motif II residue, the tellurite resistance of the mutant is partially reduced | Escherichia coli |
| F98A | site-directed mutagenesis of a motif II residue, the tellurite resistance of the mutant is abolished | Escherichia coli |
| F98Y | site-directed mutagenesis of a motif II residue, the tellurite resistance of the mutant is abolished | Escherichia coli |
| additional information | mutagenesis by the two-stage PCR-based overlapping extension method | Escherichia coli |
| Y96A | site-directed mutagenesis of a motif II residue, the tellurite resistance of the mutant is partially reduced | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Escherichia coli | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26256 | - |
x * 26256, His6-tagged TehB, sequence caculation, x * 27000-28000, recombinant His6-tagged TehB, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + tellurite | Escherichia coli | - |
S-adenosyl-L-homocysteine + methanetelluronate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P25397 | gene tehB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged wild-type TehB from Escherichia coli strains C41(DE3) and BL21(DE3), respectively, by nickel affinity chromatographhy | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + tellurite | - |
Escherichia coli | S-adenosyl-L-homocysteine + methanetelluronate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer or dimer | x * 26256, His6-tagged TehB, sequence caculation, x * 27000-28000, recombinant His6-tagged TehB, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TehB | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | dependent on, the conserved motifs I and II in TehB are involved in S-adenosyl-L-methionine binding | Escherichia coli | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | replacement of the conserved aspartate residue in motif I by asparagine or alanine, or of the conserved phenylalanine in motif II by tyrosine or alanine, decreases resistance to background levels | Escherichia coli |
| metabolism | the Escherichia coli chromosomal determinant for tellurite resistance consists of two genes tehA and tehB, which, when expressed on a multicopy plasmid, confer resistance to K2TeO3 at 0.128 mg/ml | Escherichia coli |
| physiological function | TehB utilizes a methyltransferase activity in the detoxification of tellurite | Escherichia coli |
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