Cloned (Comment) | Organism |
---|---|
gene tehB, expression as His-tagged enzyme with a TEV protease cleavage site in Escherichia coi strain BL21 (DE3) PlysS | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
TehB complexed with S-adenosyl-L-methionine or sinefungin, hanging drop vapour diffusion method, mixing of 0.001 ml of protein and 0.001 ml of reservoir solution, the latter containing 0.1 M NaF and 16-18% PEG 3350, at 20°C, formation of sinefungin complex crystals by microseeding of crystals in reservoir solution containing 5 mM sinefungin, method optimization, X-ray diffraction structure determination and analysis at 1.9 A resolution | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sinefungin | a cofactor analogue of S-adenosyl-L-methionine | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Escherichia coli | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene tehB | - |
Escherichia coli MG1655 | - |
gene tehB | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme with a TEV protease cleavage site from Escherichia coli strain BL21(DE3) PlysS by nickel affinity chromatography, cleavage of the His-tag, followed by gel filtration | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
TehB | - |
Escherichia coli |
tellurite-resistance S-adenosyl-L-methionine transferase protein | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | TehB is a SAM-dependent methyltransferase | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | TehB is an S-adenosyl-l-methionine-dependent methyltransferase that detoxifies tellurite in bacteria, the cytoplasmic TehB is a tellurite-resistance protein | Escherichia coli |