Cloned (Comment) | Organism |
---|---|
overexpression of TMT-1 and TMT-2 in Saccharomyces cerevisiae microsomes leading to mono- and dimethylated products, co-expression of various TMT and SMT-like genes in Saccharomyces cerevisiae strain TN-7 | Botryococcus braunii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten enzyme kinetics for TMT-1 and TMT-2 | Botryococcus braunii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Botryococcus braunii | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + squalene | Botryococcus braunii | overall reaction | 2 S-adenosyl-L-homocysteine + 3,22-dimethyl-1,2,23,24-tetradehydro-2,3,22,23-tetrahydrosqualene | - |
ir | |
2 S-adenosyl-L-methionine + squalene | Botryococcus braunii race B | overall reaction | 2 S-adenosyl-L-homocysteine + 3,22-dimethyl-1,2,23,24-tetradehydro-2,3,22,23-tetrahydrosqualene | - |
ir | |
S-adenosyl-L-methionine + 3-methyl-1,2-didehydro-2,3-dihydrosqualene | Botryococcus braunii | - |
S-adenosyl-L-homocysteine + 3,22-dimethyl-1,2,23,24-tetradehydro-2,3,22,23-tetrahydrosqualene | - |
? | |
S-adenosyl-L-methionine + squalene | Botryococcus braunii | - |
S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrosqualene | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Botryococcus braunii | - |
- |
- |
Botryococcus braunii race B | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.56 | - |
recombinant TMT-1, pH 7.5, 37°C | Botryococcus braunii |
143.7 | - |
recombinant TMT-2, pH 7.5, 37°C | Botryococcus braunii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + squalene | overall reaction | Botryococcus braunii | 2 S-adenosyl-L-homocysteine + 3,22-dimethyl-1,2,23,24-tetradehydro-2,3,22,23-tetrahydrosqualene | - |
ir | |
2 S-adenosyl-L-methionine + squalene | overall reaction | Botryococcus braunii race B | 2 S-adenosyl-L-homocysteine + 3,22-dimethyl-1,2,23,24-tetradehydro-2,3,22,23-tetrahydrosqualene | - |
ir | |
additional information | mono- and dimethylated products with terminal carbon C-3 as the atomic acceptor sites for the methyl additions to squalene, NMR analysis, overview TMT-1 and TMT-2 show only low activity with C30 botryococcene | Botryococcus braunii | ? | - |
? | |
additional information | mono- and dimethylated products with terminal carbon C-3 as the atomic acceptor sites for the methyl additions to squalene, NMR analysis, overview TMT-1 and TMT-2 show only low activity with C30 botryococcene | Botryococcus braunii race B | ? | - |
? | |
S-adenosyl-L-methionine + 3-methyl-1,2-didehydro-2,3-dihydrosqualene | - |
Botryococcus braunii | S-adenosyl-L-homocysteine + 3,22-dimethyl-1,2,23,24-tetradehydro-2,3,22,23-tetrahydrosqualene | - |
? | |
S-adenosyl-L-methionine + squalene | - |
Botryococcus braunii | S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrosqualene | - |
? |
Synonyms | Comment | Organism |
---|---|---|
triterpene methyltransferase | - |
Botryococcus braunii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Botryococcus braunii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Botryococcus braunii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Botryococcus braunii |
General Information | Comment | Organism |
---|---|---|
metabolism | TMT-1 and TMT-2 exhibit preference for squalene as the methyl acceptor substrate | Botryococcus braunii |