BRENDA - Enzyme Database show
show all sequences of 2.1.1.261

Partial reconstruction of the ergot alkaloid pathway by heterologous gene expression in Aspergillus nidulans

Ryan, K.L.; Moore, C.T.; Panaccione, D.G.; Toxins 5, 445-455 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene easF, a fragment of genomic DNA containing dmaW, easF, easE, and easC is amplified from the human-pathogenic, ergot alkaloid-producing fungus Aspergillus fumigatus and transformed into Aspergillus nidulans strain FGSC A767, a model fungus that does not contain any of the ergot alkaloid synthesis genes. Transformed Aspergillus nidulans strains produces chanoclavine-I and an earlier pathway intermediate
Aspergillus fumigatus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
Aspergillus fumigatus
-
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus fumigatus
Q4WZ60
gene easF
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
-
737281
Aspergillus fumigatus
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
-
-
?
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
easF encodes a methyltransferase that N-methylates DMAT to form the second intermediate, N-Me-DMAT
737281
Aspergillus fumigatus
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Aspergillus fumigatus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene easF, a fragment of genomic DNA containing dmaW, easF, easE, and easC is amplified from the human-pathogenic, ergot alkaloid-producing fungus Aspergillus fumigatus and transformed into Aspergillus nidulans strain FGSC A767, a model fungus that does not contain any of the ergot alkaloid synthesis genes. Transformed Aspergillus nidulans strains produces chanoclavine-I and an earlier pathway intermediate
Aspergillus fumigatus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Aspergillus fumigatus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
Aspergillus fumigatus
-
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
-
737281
Aspergillus fumigatus
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
-
-
?
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
easF encodes a methyltransferase that N-methylates DMAT to form the second intermediate, N-Me-DMAT
737281
Aspergillus fumigatus
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
the enzyme catalyzes the second step in the biosynthesis of ergot alkaloids, five steps of ergot alkaloid synthesis leading to the formation of chanoclavine-I, overview
Aspergillus fumigatus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme catalyzes the second step in the biosynthesis of ergot alkaloids, five steps of ergot alkaloid synthesis leading to the formation of chanoclavine-I, overview
Aspergillus fumigatus
Other publictions for EC 2.1.1.261
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736294
Ryan
Identification and structural ...
Aspergillus fumigatus
J. Agric. Food Chem.
63
61-67
2015
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737281
Ryan
Partial reconstruction of the ...
Aspergillus fumigatus
Toxins
5
445-455
2013
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719849
Rigbers
Ergot alkaloid biosynthesis in ...
Aspergillus fumigatus, Aspergillus fumigatus B5233
J. Biol. Chem.
283
26859-26868
2008
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1
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2
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1
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