BRENDA - Enzyme Database show
show all sequences of 2.1.1.261

Ergot alkaloid biosynthesis in Aspergillus fumigatus. Overproduction and biochemical characterization of a 4-dimethylallyltryptophan N-methyltransferase

Rigbers, O.; Li, S.M.; J. Biol. Chem. 283, 26859-26868 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene fgaMT, DNA and amino acid sequence determination and analysis, overexpression of the soluble His6-tagged enzyme in Escherichia coli strain XL-1 blue MRF-
Aspergillus fumigatus
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
13% inhibition at 5 mM
Aspergillus fumigatus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
4-dimethylallyl-L-tryptophan
pH 7.5, 37°C, recombinant enzyme
Aspergillus fumigatus
2.4
-
S-adenosyl-L-methionine
pH 7.5, 37°C, recombinant enzyme
Aspergillus fumigatus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
the enzyme does not require metal ions Mg2+,Mn2+, Ca2+, K+, and Na+ for its enzymatic reaction
Aspergillus fumigatus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38100
-
2 * 40000, recombinant His6-tagged enzyme, SDS-PAGE, x * 39200, about, His6-tagged enzyme, sequence calculation, x * 38100, about, non-tagged enzyme, sequence calculation
Aspergillus fumigatus
39200
-
2 * 40000, recombinant His6-tagged enzyme, SDS-PAGE, x * 39200, about, His6-tagged enzyme, sequence calculation, x * 38100, about, non-tagged enzyme, sequence calculation
Aspergillus fumigatus
40000
-
2 * 40000, recombinant His6-tagged enzyme, SDS-PAGE, x * 39200, about, His6-tagged enzyme, sequence calculation, x * 38100, about, non-tagged enzyme, sequence calculation
Aspergillus fumigatus
75000
-
recombinant His6-tagged enzyme, gel filtration
Aspergillus fumigatus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
Aspergillus fumigatus
-
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
-
?
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
Aspergillus fumigatus B5233
-
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus fumigatus
B6D5I7
gene fgaMT, ATCC 13073
-
Aspergillus fumigatus B5233
B6D5I7
gene fgaMT, ATCC 13073
-
Purification (Commentary)
Commentary
Organism
recombinant soluble His6-tagged enzyme in Escherichia coli strain XL-1 blue MRF- by nickel affinity chromatography and gel filtration to apparent homogeneity
Aspergillus fumigatus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme shows a relatively high specificity toward the prenyl moiety at position C-4 of the indole ring, 4-dimethylallyltryptophan derivatives with modification at the indole ring are also accepted by FgaMT as substrates
719849
Aspergillus fumigatus
?
-
-
-
-
additional information
the enzyme shows a relatively high specificity toward the prenyl moiety at position C-4 of the indole ring, 4-dimethylallyltryptophan derivatives with modification at the indole ring are also accepted by FgaMT as substrates
719849
Aspergillus fumigatus B5233
?
-
-
-
-
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
-
719849
Aspergillus fumigatus
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
-
-
?
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
the enzyme shows a relatively high specificity toward the prenyl moiety at position C-4 of the indole ring
719849
Aspergillus fumigatus
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
product identificaation by NMR and mass spectrometry analysis
-
-
?
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
-
719849
Aspergillus fumigatus B5233
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
-
-
?
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
the enzyme shows a relatively high specificity toward the prenyl moiety at position C-4 of the indole ring
719849
Aspergillus fumigatus B5233
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
product identificaation by NMR and mass spectrometry analysis
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 40000, recombinant His6-tagged enzyme, SDS-PAGE, x * 39200, about, His6-tagged enzyme, sequence calculation, x * 38100, about, non-tagged enzyme, sequence calculation
Aspergillus fumigatus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Aspergillus fumigatus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2
-
4-dimethylallyl-L-tryptophan
pH 7.5, 37°C, recombinant enzyme
Aspergillus fumigatus
2
-
S-adenosyl-L-methionine
pH 7.5, 37°C, recombinant enzyme
Aspergillus fumigatus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Aspergillus fumigatus
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Aspergillus fumigatus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene fgaMT, DNA and amino acid sequence determination and analysis, overexpression of the soluble His6-tagged enzyme in Escherichia coli strain XL-1 blue MRF-
Aspergillus fumigatus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Aspergillus fumigatus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
13% inhibition at 5 mM
Aspergillus fumigatus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
4-dimethylallyl-L-tryptophan
pH 7.5, 37°C, recombinant enzyme
Aspergillus fumigatus
2.4
-
S-adenosyl-L-methionine
pH 7.5, 37°C, recombinant enzyme
Aspergillus fumigatus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
the enzyme does not require metal ions Mg2+,Mn2+, Ca2+, K+, and Na+ for its enzymatic reaction
Aspergillus fumigatus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38100
-
2 * 40000, recombinant His6-tagged enzyme, SDS-PAGE, x * 39200, about, His6-tagged enzyme, sequence calculation, x * 38100, about, non-tagged enzyme, sequence calculation
Aspergillus fumigatus
39200
-
2 * 40000, recombinant His6-tagged enzyme, SDS-PAGE, x * 39200, about, His6-tagged enzyme, sequence calculation, x * 38100, about, non-tagged enzyme, sequence calculation
Aspergillus fumigatus
40000
-
2 * 40000, recombinant His6-tagged enzyme, SDS-PAGE, x * 39200, about, His6-tagged enzyme, sequence calculation, x * 38100, about, non-tagged enzyme, sequence calculation
Aspergillus fumigatus
75000
-
recombinant His6-tagged enzyme, gel filtration
Aspergillus fumigatus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
Aspergillus fumigatus
-
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
-
?
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
Aspergillus fumigatus B5233
-
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant soluble His6-tagged enzyme in Escherichia coli strain XL-1 blue MRF- by nickel affinity chromatography and gel filtration to apparent homogeneity
Aspergillus fumigatus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme shows a relatively high specificity toward the prenyl moiety at position C-4 of the indole ring, 4-dimethylallyltryptophan derivatives with modification at the indole ring are also accepted by FgaMT as substrates
719849
Aspergillus fumigatus
?
-
-
-
-
additional information
the enzyme shows a relatively high specificity toward the prenyl moiety at position C-4 of the indole ring, 4-dimethylallyltryptophan derivatives with modification at the indole ring are also accepted by FgaMT as substrates
719849
Aspergillus fumigatus B5233
?
-
-
-
-
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
-
719849
Aspergillus fumigatus
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
-
-
?
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
the enzyme shows a relatively high specificity toward the prenyl moiety at position C-4 of the indole ring
719849
Aspergillus fumigatus
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
product identificaation by NMR and mass spectrometry analysis
-
-
?
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
-
719849
Aspergillus fumigatus B5233
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
-
-
?
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
the enzyme shows a relatively high specificity toward the prenyl moiety at position C-4 of the indole ring
719849
Aspergillus fumigatus B5233
S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
product identificaation by NMR and mass spectrometry analysis
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 40000, recombinant His6-tagged enzyme, SDS-PAGE, x * 39200, about, His6-tagged enzyme, sequence calculation, x * 38100, about, non-tagged enzyme, sequence calculation
Aspergillus fumigatus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Aspergillus fumigatus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2
-
4-dimethylallyl-L-tryptophan
pH 7.5, 37°C, recombinant enzyme
Aspergillus fumigatus
2
-
S-adenosyl-L-methionine
pH 7.5, 37°C, recombinant enzyme
Aspergillus fumigatus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Aspergillus fumigatus
General Information
General Information
Commentary
Organism
metabolism
FgaMT represents the second pathway-specific enzyme in the biosynthesis of ergot alkaloids
Aspergillus fumigatus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
FgaMT represents the second pathway-specific enzyme in the biosynthesis of ergot alkaloids
Aspergillus fumigatus
Other publictions for EC 2.1.1.261
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736294
Ryan
Identification and structural ...
Aspergillus fumigatus
J. Agric. Food Chem.
63
61-67
2015
-
1
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
737281
Ryan
Partial reconstruction of the ...
Aspergillus fumigatus
Toxins
5
445-455
2013
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
719849
Rigbers
Ergot alkaloid biosynthesis in ...
Aspergillus fumigatus, Aspergillus fumigatus B5233
J. Biol. Chem.
283
26859-26868
2008
-
-
1
-
-
-
1
2
-
1
4
2
-
4
-
-
1
-
-
-
-
-
6
1
1
-
-
2
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
2
-
1
4
2
-
-
-
1
-
-
-
-
6
1
1
-
-
2
1
-
-
-
-
1
1
-
-
-