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Literature summary for 2.1.1.260 extracted from
- Nep1p (Emg1p), a novel protein conserved in eukaryotes and archaea, is involved in ribosome biogenesis (2002), Curr. Genet., 40, 326-338.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene NEP1, DNA and amino acid sequence determination and analysis | Candida albicans |
| gene NEP1, DNA and amino acid sequence determination and analysis | [Candida] glabrata |
| gene NEP1, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of the GFP-tagged ScNEP1 in enzyme-deficient Saccharomyces cerevisiae strain CEN.SR679 in the nucleus | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | complementation of a ScDnep1 strain with the human Nep1 (C2F) protein, the HsNEP1 open reading frame is expressed with the yeast inducible/repressible GAL1 promoter and the resulting plasmid (pGALHsNEP1) is transformed into the heterozygous ScDnep1/ScNEP1 strain CEN.SR679. After sporulation and tetrad analysis, ScDnep1 segregants complemented by the pGAL-HsNEP1 are only viable with galactose | Homo sapiens |
| additional information | complementation of a ScDnep1 strain with the human Nep1 (C2F) protein, the HsNEP1 open reading frame is expressed with the yeast inducible/repressible GAL1 promoter and the resulting plasmid (pGALHsNEP1) is transformed into the heterozygous ScDnep1/ScNEP1 strain CEN.SR679. After sporulation and tetrad analysis, ScDnep1 segregants complemented by the pGAL-HsNEP1 are only viable with galactose | Saccharomyces cerevisiae |
| additional information | construction of a heterozygous CaDnep1/CaNEP1 strain CAE8 after replacement of one CaNEP1 wild-type allele with a CaURA3 marker and introduction of GFP-open reading frame driven by the methionine/cysteine-repressible CaMET3 promoter in front of the gene. Without high concentrations of methionine and cysteine in the medium, the resulting strain is viable, but addition of 2.5 mM methionine and 2.5 mM cysteine strongly impairs growth | Candida albicans |
| additional information | construction of a heterozygous CaDnep1/CaNEP1 strain CAE8 after replacement of one CaNEP1 wild-type allele with a CaURA3 marker and introduction of GFP-open reading frame driven by the methionine/cysteine-repressible CaMET3 promoter in front of the gene. Without high concentrationsof methionine and cysteine in the medium, the resulting strain is viable, but addition of 2.5 mM methionine and 2.5 mM cysteine strongly impairs growth | [Candida] glabrata |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microtubule | the spindle/microtubule association is specific for ScNep1p | Saccharomyces cerevisiae | 5874 | - |
| nucleolus | - |
Homo sapiens | 5730 | - |
| nucleolus | - |
Candida albicans | 5730 | - |
| nucleolus | - |
[Candida] glabrata | 5730 | - |
| spindle | the spindle/microtubule association is specific for ScNep1p | Saccharomyces cerevisiae | 5819 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
x * 28000 | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA | - |
? |  |
| S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA | Saccharomyces cerevisiae CEN.PK2 | - |
S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | Q9P8P7 | derived from the clinical isolate SC5314, gene NEP1 | - |
| Candida albicans RM1000 | Q9P8P7 | derived from the clinical isolate SC5314, gene NEP1 | - |
| Homo sapiens | - |
gene NEP1 | - |
| Saccharomyces cerevisiae | - |
and isogenic strains, gene YLR186w or NEP1 or EMG1 | - |
| Saccharomyces cerevisiae CEN.PK2 | - |
and isogenic strains, gene YLR186w or NEP1 or EMG1 | - |
| [Candida] glabrata | Q96UP2 | derived from the clinical isolate SC5314, gene NEP1 | - |
| [Candida] glabrata CBS 138 | Q96UP2 | derived from the clinical isolate SC5314, gene NEP1 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA | - |
? | |
| S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA | - |
Saccharomyces cerevisiae CEN.PK2 | S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28000 | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Emg1p | - |
Homo sapiens |
| Emg1p | - |
Saccharomyces cerevisiae |
| Emg1p | - |
Candida albicans |
| Emg1p | - |
[Candida] glabrata |
| essential for mitotic growth 1 | - |
Homo sapiens |
| essential for mitotic growth 1 | - |
Saccharomyces cerevisiae |
| essential for mitotic growth 1 | - |
Candida albicans |
| essential for mitotic growth 1 | - |
[Candida] glabrata |
| Nep1p | - |
Homo sapiens |
| Nep1p | - |
Saccharomyces cerevisiae |
| Nep1p | - |
Candida albicans |
| Nep1p | - |
[Candida] glabrata |
| nucleolar essential protein 1 | - |
Homo sapiens |
| nucleolar essential protein 1 | - |
Saccharomyces cerevisiae |
| nucleolar essential protein 1 | - |
Candida albicans |
| nucleolar essential protein 1 | - |
[Candida] glabrata |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Homo sapiens | |
| S-adenosyl-L-methionine | - |
Saccharomyces cerevisiae | |
| S-adenosyl-L-methionine | - |
Candida albicans | |
| S-adenosyl-L-methionine | - |
[Candida] glabrata | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | a temperature-sensitive ScNEP1ts1 allele is isolated and reveals a strongly increased sensitivity to paromomycin, a translational inhibitor which binds to RNA, indicating that ribosome biogenesis within the nucleolus is probably affected. Candida albicans and human NEP1 heterologously complement the essential phenotype in a Saccharomyces cerevisiae nep1 deletion mutant, the ScNEP1 spindle/microtubule phenotype is not found with HsNEP1 and CaNEP1 | Saccharomyces cerevisiae |
| additional information | Candida albicans NEP1 heterologously complements the essential phenotype in a Saccharomyces cerevisiae nep1 deletion mutant | Candida albicans |
| additional information | human NEP1 heterologously complements the essential phenotype in a Saccharomyces cerevisiae nep1 deletion mutant | Homo sapiens |
| physiological function | the rRNA modifications are thought to play a role in modulation of the three-dimensional structure of RNA and in fine-tuning its interactions with other RNAs or proteins | Homo sapiens |
| physiological function | the rRNA modifications are thought to play a role in modulation of the three-dimensional structure of RNA and in fine-tuning its interactions with other RNAs or proteins | Candida albicans |
| physiological function | the rRNA modifications are thought to play a role in modulation of the three-dimensional structure of RNA and in fine-tuning its interactions with other RNAs or proteins | [Candida] glabrata |
| physiological function | the rRNA modifications are thought to play a role in modulation of the three-dimensional structure of RNA and in fine-tuning its interactions with other RNAs or proteins. The protein has an essential function in ribosomal biogenesis which directly or indirectly interferes with a methylation reaction during the early steps of pre-rRNA processing necessary for the generation of 40S ribosomal subunits | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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