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Literature summary for 2.1.1.258 extracted from

  • Zhu, X.; Li, T.; Gu, X.; Zhang, S.; Liu, Y.; Wang, Y.; Tan, X.
    Structural and functional investigation into acetyl-coenzyme A synthase and methyltransferase from human pathogen Clostridium difficile (2013), Metallomics, 5, 551-558.
    View publication on PubMed
Search for more literature for 2.1.1.258

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Clostridioides difficile

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0178
-
 Cobalamin 25°C, pH 5.1 Clostridioides difficile
0.06
-
 Cobalamin 25°C, pH 7.2 Clostridioides difficile

Organism

Organism UniProt Comment Textmining
Clostridioides difficile
-
-
-
Clostridioides difficile 630
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cobalamin + 5-methyltetrahydrofolate reaction is pH dependent with the best pH of around 5.0 Clostridioides difficile methylcobalamin + tetrahydrofolate
-
 ?
cobalamin + 5-methyltetrahydrofolate reaction is pH dependent with the best pH of around 5.0 Clostridioides difficile 630 methylcobalamin + tetrahydrofolate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.63
-
 Cobalamin 25°C, pH 5.1 Clostridioides difficile
37.2
-
 Cobalamin 25°C, pH 7.2 Clostridioides difficile

pI Value

Organism Comment pI Value Maximum pI Value
Clostridioides difficile calculated
-
 4.8

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
150
-
 Cobalamin 25°C, pH 5.1 Clostridioides difficile
620
-
 Cobalamin 25°C, pH 7.2 Clostridioides difficile