Any feedback?
Literature summary for 2.1.1.258 extracted from
- Efficient expression and purification of methyltransferase in acetyl-coenzyme a synthesis pathway of the human pathogen Clostridium difficile (2011), Protein Expr. Purif., 78, 86-93.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta (DE3) pLysS cells | Clostridioides difficile |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.058 | - |
hydroxycobalamin | recombinant enzyme, in 0.1 M MES, at pH 5.1 and 25°C | Clostridioides difficile |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
2 * 30000, SDS-PAGE | Clostridioides difficile |
| 31000 | - |
2 * 31000, calculated from amino acid sequence | Clostridioides difficile |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridioides difficile | - |
- |
- |
| Clostridioides difficile 630 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| amylose resin column chromatography, Ni-NTA column chromatography, Sephadex-G25 gel filtration, and Superdex 75 gel filtration | Clostridioides difficile |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroxycobalamin + 5-methyltetrahydrofolate | - |
Clostridioides difficile | methylcobalamin + tetrahydrofolate | - |
? | |
| hydroxycobalamin + 5-methyltetrahydrofolate | - |
Clostridioides difficile 630 | methylcobalamin + tetrahydrofolate | - |
? | |
| [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrofolate | - |
Clostridioides difficile | [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrofolate | - |
? | |
| [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrofolate | - |
Clostridioides difficile 630 | [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrofolate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 30000, SDS-PAGE | Clostridioides difficile |
| homodimer | 2 * 31000, calculated from amino acid sequence | Clostridioides difficile |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methyltetrahydrofolate- and corrinoid-dependent methyltransferase | - |
Clostridioides difficile |
| MeTr | - |
Clostridioides difficile |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 16 | - |
the recombinant enzyme is not stable at 16°C or higher temperature | Clostridioides difficile |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 77.6 | - |
hydroxycobalamin | recombinant enzyme, in 0.1 M MES, at pH 5.1 and 25°C | Clostridioides difficile | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1330 | - |
hydroxycobalamin | recombinant enzyme, in 0.1 M MES, at pH 5.1 and 25°C | Clostridioides difficile | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
