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Literature summary for 2.1.1.258 extracted from
- The reductive acetyl coenzyme A pathway: Sequence and heterologous expression of active methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase from Clostridium thermoaceticum (1994), J. Bacteriol., 176, 6127-6130.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain JM109 | Moorella thermoacetica |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme lacks any metals | Moorella thermoacetica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
2 * 27000, SDS-PAGE | Moorella thermoacetica |
| 28550 | - |
2 * 28550, recombinant enzyme, MALDI-TOF mass spectrometry | Moorella thermoacetica |
| 28640 | - |
2 * 28640, native enzyme, MALDI-TOF mass spectrometry | Moorella thermoacetica |
| 28641 | - |
2 * 28641, deduced from amino acid sequence | Moorella thermoacetica |
| 58900 | - |
native enzyme, gel filtration | Moorella thermoacetica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Moorella thermoacetica | Q46389 | formerly Clostridium thermoaceticum | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE column chromatography | Moorella thermoacetica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrofolate | - |
Moorella thermoacetica | [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrofolate | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 27000, SDS-PAGE | Moorella thermoacetica |
| homodimer | 2 * 28550, recombinant enzyme, MALDI-TOF mass spectrometry | Moorella thermoacetica |
| homodimer | 2 * 28640, native enzyme, MALDI-TOF mass spectrometry | Moorella thermoacetica |
| homodimer | 2 * 28641, deduced from amino acid sequence | Moorella thermoacetica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acsE | - |
Moorella thermoacetica |
| methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase | - |
Moorella thermoacetica |
| MeTr | - |
Moorella thermoacetica |
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Release 2023.1 (January 2023)
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