BRENDA - Enzyme Database
show all sequences of 2.1.1.256

Formation of m2G6 in Methanocaldococcus jannaschii tRNA catalyzed by the novel methyltransferase Trm14

Menezes, S.; Gaston, K.W.; Krivos, K.L.; Apolinario, E.E.; Reich, N.O.; Sowers, K.R.; Limbach, P.A.; Perona, J.J.; Nucleic Acids Res. 39, 7641-7655 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene MJ0438 or trm14, DNA and amino acid sequence determination, analysis and comparisons, phylogenetic analysis
Methanocaldococcus jannaschii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
single-turnover, kinetic analysis, overview
Methanocaldococcus jannaschii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine6 in tRNACys
Methanocaldococcus jannaschii
Trm14 generates m2G at position 6 in tRNACys
S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNACys
-
-
?
S-adenosyl-L-methionine + guanine6 in tRNACys
Methanocaldococcus jannaschii JAL-1
Trm14 generates m2G at position 6 in tRNACys
S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNACys
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanocaldococcus jannaschii
Q57880
DSM 2661, gene MJ0438 or trm14
-
Methanocaldococcus jannaschii JAL-1
Q57880
DSM 2661, gene MJ0438 or trm14
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine6 in tRNACys
Trm14 generates m2G at position 6 in tRNACys
720590
Methanocaldococcus jannaschii
S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNACys
-
-
-
?
S-adenosyl-L-methionine + guanine6 in tRNACys
wild-type or G6A/C67U tRNACys transcripts as substrates, Trm14 generates m2G at position 6 in tRNACys, i.e. tRNACys m2G6-forming activity
720590
Methanocaldococcus jannaschii
S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNACys
-
-
-
?
S-adenosyl-L-methionine + guanine6 in tRNACys
Trm14 generates m2G at position 6 in tRNACys
720590
Methanocaldococcus jannaschii JAL-1
S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNACys
-
-
-
?
S-adenosyl-L-methionine + guanine6 in tRNACys
wild-type or G6A/C67U tRNACys transcripts as substrates, Trm14 generates m2G at position 6 in tRNACys, i.e. tRNACys m2G6-forming activity
720590
Methanocaldococcus jannaschii JAL-1
S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNACys
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
the 381 amino acid Trm14 protein possesses a canonical RNA recognition THUMP domain at the N-terminus, followed by a gamma-class Rossmann fold amino-methyltransferase catalytic domain featuring the signature NPPY active site motif
Methanocaldococcus jannaschii
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Methanocaldococcus jannaschii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
assay at
Methanocaldococcus jannaschii
Cloned(Commentary) (protein specific)
Commentary
Organism
gene MJ0438 or trm14, DNA and amino acid sequence determination, analysis and comparisons, phylogenetic analysis
Methanocaldococcus jannaschii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
single-turnover, kinetic analysis, overview
Methanocaldococcus jannaschii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine6 in tRNACys
Methanocaldococcus jannaschii
Trm14 generates m2G at position 6 in tRNACys
S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNACys
-
-
?
S-adenosyl-L-methionine + guanine6 in tRNACys
Methanocaldococcus jannaschii JAL-1
Trm14 generates m2G at position 6 in tRNACys
S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNACys
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine6 in tRNACys
Trm14 generates m2G at position 6 in tRNACys
720590
Methanocaldococcus jannaschii
S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNACys
-
-
-
?
S-adenosyl-L-methionine + guanine6 in tRNACys
wild-type or G6A/C67U tRNACys transcripts as substrates, Trm14 generates m2G at position 6 in tRNACys, i.e. tRNACys m2G6-forming activity
720590
Methanocaldococcus jannaschii
S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNACys
-
-
-
?
S-adenosyl-L-methionine + guanine6 in tRNACys
Trm14 generates m2G at position 6 in tRNACys
720590
Methanocaldococcus jannaschii JAL-1
S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNACys
-
-
-
?
S-adenosyl-L-methionine + guanine6 in tRNACys
wild-type or G6A/C67U tRNACys transcripts as substrates, Trm14 generates m2G at position 6 in tRNACys, i.e. tRNACys m2G6-forming activity
720590
Methanocaldococcus jannaschii JAL-1
S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNACys
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
the 381 amino acid Trm14 protein possesses a canonical RNA recognition THUMP domain at the N-terminus, followed by a gamma-class Rossmann fold amino-methyltransferase catalytic domain featuring the signature NPPY active site motif
Methanocaldococcus jannaschii
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Methanocaldococcus jannaschii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
assay at
Methanocaldococcus jannaschii
General Information
General Information
Commentary
Organism
evolution
Trm14 is associated with cluster of orthologous groups, COG, 0116, and most closely resembles the m2G10 tRNA methylase Trm11. Phylogenetic analysis reveals a canonical archaeal/bacterial evolutionary separation with 20-30% sequence identities between the two branches, but it is likely that the detailed functions of COG 0116 enzymes differ between the archaeal and bacterial domains. Phylogenetic distribution of Trm14-like proteins, overview
Methanocaldococcus jannaschii
physiological function
tRNA m2G6 formation may play a role in stabilizing the structure of the RNA and function synergistically with these core-region modifications to stabilize the molecule against thermal stress. Also, Trm14-catalyzed m2G6 formation in tRNACys may play a role in modulating the aminoacylation efficiency of phosphoseryl-tRNA synthetase, i.e. SepRS. Control of SepRS (or CysRS) activity by SAM-dependent methylation of tRNACys might provide the methanogen cell with a regulatory mechanism by which protein synthesis rates can respond to environmental sulfur levels or to concentrations of sulfur metabolites
Methanocaldococcus jannaschii
General Information (protein specific)
General Information
Commentary
Organism
evolution
Trm14 is associated with cluster of orthologous groups, COG, 0116, and most closely resembles the m2G10 tRNA methylase Trm11. Phylogenetic analysis reveals a canonical archaeal/bacterial evolutionary separation with 20-30% sequence identities between the two branches, but it is likely that the detailed functions of COG 0116 enzymes differ between the archaeal and bacterial domains. Phylogenetic distribution of Trm14-like proteins, overview
Methanocaldococcus jannaschii
physiological function
tRNA m2G6 formation may play a role in stabilizing the structure of the RNA and function synergistically with these core-region modifications to stabilize the molecule against thermal stress. Also, Trm14-catalyzed m2G6 formation in tRNACys may play a role in modulating the aminoacylation efficiency of phosphoseryl-tRNA synthetase, i.e. SepRS. Control of SepRS (or CysRS) activity by SAM-dependent methylation of tRNACys might provide the methanogen cell with a regulatory mechanism by which protein synthesis rates can respond to environmental sulfur levels or to concentrations of sulfur metabolites
Methanocaldococcus jannaschii
Other publictions for EC 2.1.1.256
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
720602
Fislage
Crystal structures of the tRNA ...
Pyrococcus furiosus, Thermus thermophilus
Nucleic Acids Res.
40
5149-5161
2012
-
-
2
2
-
-
2
-
-
2
-
2
-
7
-
-
-
-
-
-
-
-
6
2
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
-
2
-
2
-
-
-
-
-
-
-
-
6
2
2
-
-
-
2
-
-
-
-
2
2
-
-
-
746421
Roovers
The open reading frame TTC115 ...
Pyrococcus furiosus, Thermus thermophilus, Thermus thermophilus DSM 7039
RNA
18
815-824
2012
-
-
2
-
-
-
-
-
-
-
-
-
-
6
-
-
2
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
720590
Menezes
Formation of m2G6 in Methanoca ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii JAL-1
Nucleic Acids Res.
39
7641-7655
2011
-
-
1
-
-
-
-
1
-
1
-
2
-
4
-
-
-
-
-
-
-
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
-
4
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-