Cloned (Comment) | Organism |
---|---|
gene mtsA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH5alpha. The gene encoding the alpha subunit of the CoM methylase, mtsA, is 5' to the gene encoding the beta subunit, mtsB, regulated cotranscription of mtsA and mtsB | Methanosarcina barkeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
480000 | - |
- |
Methanosarcina barkeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + coenzyme M | Methanosarcina barkeri | - |
methyl-CoM + a [Co(I) methylated-thiol-specific corrinoid protein] | - |
? | |
methanethiol + a [Co(I) methylated-thiol-specific corrinoid protein] | Methanosarcina barkeri | - |
a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + hydrogen sulfide | - |
? | |
methanethiol + coenzyme M | Methanosarcina barkeri | - |
methyl-CoM + hydrogen sulfide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | Q48924 | gene mtsA | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + CoM = methyl-CoM + a [Co(I) methylated-thiol-specific corrinoid protein] | mechanism of corrinoid binding by proteins involved in methylotrophic methanogenesis | Methanosarcina barkeri |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the mtsA transcript is most abundant in cells grown to the late log phase on acetate but barely detectable in cells grown on methanol or trimethylamine, overview | Methanosarcina barkeri | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + coenzyme M | - |
Methanosarcina barkeri | methyl-CoM + a [Co(I) methylated-thiol-specific corrinoid protein] | - |
? | |
methanethiol + a [Co(I) methylated-thiol-specific corrinoid protein] | - |
Methanosarcina barkeri | a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + hydrogen sulfide | - |
? | |
methanethiol + coenzyme M | - |
Methanosarcina barkeri | methyl-CoM + hydrogen sulfide | - |
? | |
additional information | the 480-kDa protein is not methylated by methyltetrahydrosarcinapterin or demethylated by homocysteine. The alpha subunit, not the beta subunit, methylates the thiol of CoM | Methanosarcina barkeri | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methyltransferase II | - |
Methanosarcina barkeri |
MtsA | - |
Methanosarcina barkeri |
General Information | Comment | Organism |
---|---|---|
evolution | the sequence of MtsA is homologous to the A and M isozymes of methylcobamide:coenzyme M methyltransferases (methyltransferase II), indicating that the alpha polypeptide is a member of the methyltransferase II family of coenzyme M methylases | Methanosarcina barkeri |