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Literature summary for 2.1.1.251 extracted from

  • Paul, L.; Krzycki, J.
    Sequence and transcript analysis of a novel Methanosarcina barkeri methyltransferase II homolog and its associated corrinoid protein homologous to methionine synthase (1996), J. Bacteriol., 178, 6599-6607.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene mtsA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH5alpha. The gene encoding the alpha subunit of the CoM methylase, mtsA, is 5' to the gene encoding the beta subunit, mtsB, regulated cotranscription of mtsA and mtsB Methanosarcina barkeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
480000
-
-
Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + coenzyme M Methanosarcina barkeri
-
methyl-CoM + a [Co(I) methylated-thiol-specific corrinoid protein]
-
?
methanethiol + a [Co(I) methylated-thiol-specific corrinoid protein] Methanosarcina barkeri
-
a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + hydrogen sulfide
-
?
methanethiol + coenzyme M Methanosarcina barkeri
-
methyl-CoM + hydrogen sulfide
-
?

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri Q48924 gene mtsA
-

Reaction

Reaction Comment Organism Reaction ID
a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + CoM = methyl-CoM + a [Co(I) methylated-thiol-specific corrinoid protein] mechanism of corrinoid binding by proteins involved in methylotrophic methanogenesis Methanosarcina barkeri

Source Tissue

Source Tissue Comment Organism Textmining
additional information the mtsA transcript is most abundant in cells grown to the late log phase on acetate but barely detectable in cells grown on methanol or trimethylamine, overview Methanosarcina barkeri
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + coenzyme M
-
Methanosarcina barkeri methyl-CoM + a [Co(I) methylated-thiol-specific corrinoid protein]
-
?
methanethiol + a [Co(I) methylated-thiol-specific corrinoid protein]
-
Methanosarcina barkeri a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + hydrogen sulfide
-
?
methanethiol + coenzyme M
-
Methanosarcina barkeri methyl-CoM + hydrogen sulfide
-
?
additional information the 480-kDa protein is not methylated by methyltetrahydrosarcinapterin or demethylated by homocysteine. The alpha subunit, not the beta subunit, methylates the thiol of CoM Methanosarcina barkeri ?
-
?

Synonyms

Synonyms Comment Organism
methyltransferase II
-
Methanosarcina barkeri
MtsA
-
Methanosarcina barkeri

General Information

General Information Comment Organism
evolution the sequence of MtsA is homologous to the A and M isozymes of methylcobamide:coenzyme M methyltransferases (methyltransferase II), indicating that the alpha polypeptide is a member of the methyltransferase II family of coenzyme M methylases Methanosarcina barkeri