Cloned (Comment) | Organism |
---|---|
gene mtbB | Methanosarcina barkeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylamine + a [Co(I) methylamine-specific corrinoid protein] | Methanosarcina barkeri | - |
a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
dimethylamine + a [Co(I) methylamine-specific corrinoid protein] | Methanosarcina barkeri MS / DSM 800 | - |
a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | Q9P9N0 | gene mtbB | - |
Methanosarcina barkeri MS / DSM 800 | Q9P9N0 | gene mtbB | - |
Purification (Comment) | Organism |
---|---|
native enzyme by three different steps of anion exchange chromatography, adsorption and hydrophobic interaction chromatography | Methanosarcina barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | - |
Methanosarcina barkeri | a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | - |
Methanosarcina barkeri MS / DSM 800 | a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
dimethylamine + a [Co(I) methylamine-specific corrinoid protein] | - |
Methanosarcina barkeri | a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
dimethylamine + a [Co(I) methylamine-specific corrinoid protein] | - |
Methanosarcina barkeri MS / DSM 800 | a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | mass spectrometric structure analysis, overview, primary structure of MtbB | Methanosarcina barkeri |
Synonyms | Comment | Organism |
---|---|---|
dimethylamine:CoM methyltransferase | - |
Methanosarcina barkeri |
DMA methyltransferase | - |
Methanosarcina barkeri |
MtbB | - |
Methanosarcina barkeri |
General Information | Comment | Organism |
---|---|---|
additional information | single in-frame amber UAG codons are found in the genes encoding MtmB, MtbB, or MttB, the methyltransferases initiating methane formation from monomethylamine, dimethylamine, or trimethylamine, respectively, in certain Archaea. The amber codon codes for pyrrolysine, the 22nd genetically encoded amino acid found in nature | Methanosarcina barkeri |