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Literature summary for 2.1.1.247 extracted from

  • Ferguson Jr., D.; Krzycki, J.
    Reconstitution of trimethylamine-dependent coenzyme M methylation with the trimethylamine corrinoid protein and the isozymes of methyltransferase II from Methanosarcina barkeri (1997), J. Bacteriol., 179, 846-852.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.247

Activating Compound

Activating Compound Comment Organism Structure
methyl viologen strictly dependent on Methanosarcina barkeri
additional information stimulation of the TMA:CoM methyl transfer reaction in cell extracts Methanosarcina barkeri
Ti(III) citrate strictly dependent on Methanosarcina barkeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 1 * 52000, SDS-PAGE Methanosarcina barkeri
52900
-
 gel filtration and native PAGE Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Methanosarcina barkeri corrinoid proteins mediating CoM methylation from dimethylamine or monomethylamine have a specific requirement for MT2-A, reconstitution of trimethylamine-dependent coenzyme M methylation, overview. Trimethylamine methyl transfer can interact with either isozyme of MT2 but has the greatest affinity for the A isozyme. The predominant isozyme of MT2 from in TMA-grown cells is the favored participant in the TMA:CoM methyl transfer reaction ?
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri O30640 amine isozyme MT2-A, gene mtbA
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by two different steps of anion exchange chromatography, gel filtration, and another step of anion exchange chromatography, co-purification with the 26-kDa the trimethylamine-specific corrinoid protein Methanosarcina barkeri

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:trimethylamine-grown cell
-
 Methanosarcina barkeri
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7
-
 isozyme MT2-A, pH 7.0, 37°C Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a [methyl-Co(III) methylamine-specific corrinoid protein] + coenzyme M trimethylamine methyl transfer required, neither dimethylamine nor monomethylamine serve as substrate Methanosarcina barkeri methyl-CoM + a [Co(I) methylamine-specific corrinoid protein]
-
 ?
additional information corrinoid proteins mediating CoM methylation from dimethylamine or monomethylamine have a specific requirement for MT2-A, reconstitution of trimethylamine-dependent coenzyme M methylation, overview. Trimethylamine methyl transfer can interact with either isozyme of MT2 but has the greatest affinity for the A isozyme. The predominant isozyme of MT2 from in TMA-grown cells is the favored participant in the TMA:CoM methyl transfer reaction Methanosarcina barkeri ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 52000, SDS-PAGE Methanosarcina barkeri

Synonyms

Synonyms Comment Organism
methylcorrinoid:CoM methyltransferase
-
 Methanosarcina barkeri
methyltransferase II
-
 Methanosarcina barkeri
MT2
-
 Methanosarcina barkeri
TMA:CoM methyltransferase
-
 Methanosarcina barkeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Methanosarcina barkeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Methanosarcina barkeri

General Information

General Information Comment Organism
metabolism involvement of MT2-A in monomethylamine metabolism Methanosarcina barkeri