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Literature summary for 2.1.1.246 extracted from

  • Krüer, M.; Haumann, M.; Meyer-Klaucke, W.; Thauer, R.; Dau, H.
    The role of zinc in the methylation of the coenzyme M thiol group in methanol:coenzyme M methyltransferase from Methanosarcina barkeri: new insights from X-ray absorption spectroscopy (2002), Eur. J. Biochem., 269, 2117-2123.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene mtaA, sequence comparison, expression of His-tagged MtaA in Escherichia coli strain M15 Methanosarcina barkeri

Protein Variants

Protein Variants Comment Organism
C239A site-directed mutagenesis, mutation of the residue involved in zinc coordination in MtaA results in reduced zinc content and reduced activity compared to the wild-type enzyme Methanosarcina barkeri
H237A site-directed mutagenesis, mutation of the residue involved in zinc coordination in MtaA results in reduced zinc content and reduced activity compared to the wild-type enzyme Methanosarcina barkeri

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on, wild-type MtaA has a zinc content of 0.91 mol/mol, binding structure, overview Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M Methanosarcina barkeri
-
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
r
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M Methanosarcina barkeri Fusaro, DSM 804
-
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
r
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M Methanosarcina barkeri Fusaro / DSM 804
-
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
r

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri
-
DSM 804, gene mtaA
-
Methanosarcina barkeri Fusaro / DSM 804
-
DSM 804, gene mtaA
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
purified recombinant mutant C239A MtaA, pH 7.0, 37°C Methanosarcina barkeri
0.02
-
purified recombinant mutant H237A MtaA, pH 7.0, 37°C Methanosarcina barkeri
0.3
-
purified recombinant wild-type MtaA, pH 7.0, 37°C Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
-
Methanosarcina barkeri methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
r
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
-
Methanosarcina barkeri Fusaro, DSM 804 methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
r
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
-
Methanosarcina barkeri Fusaro / DSM 804 methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
r

Synonyms

Synonyms Comment Organism
methanol:coenzyme M methyltransferase
-
Methanosarcina barkeri
mtaA
-
Methanosarcina barkeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Methanosarcina barkeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Methanosarcina barkeri