BRENDA - Enzyme Database
show all sequences of 2.1.1.246

The role of zinc in the methylation of the coenzyme M thiol group in methanol:coenzyme M methyltransferase from Methanosarcina barkeri: new insights from X-ray absorption spectroscopy

Krüer, M.; Haumann, M.; Meyer-Klaucke, W.; Thauer, R.; Dau, H.; Eur. J. Biochem. 269, 2117-2123 (2002)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene mtaA, sequence comparison, expression of His-tagged MtaA in Escherichia coli strain M15
Methanosarcina barkeri
Engineering
Amino acid exchange
Commentary
Organism
C239A
site-directed mutagenesis, mutation of the residue involved in zinc coordination in MtaA results in reduced zinc content and reduced activity compared to the wild-type enzyme
Methanosarcina barkeri
H237A
site-directed mutagenesis, mutation of the residue involved in zinc coordination in MtaA results in reduced zinc content and reduced activity compared to the wild-type enzyme
Methanosarcina barkeri
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
dependent on, wild-type MtaA has a zinc content of 0.91 mol/mol, binding structure, overview
Methanosarcina barkeri
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
Methanosarcina barkeri
-
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
-
r
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
Methanosarcina barkeri Fusaro, DSM 804
-
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
-
r
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
Methanosarcina barkeri Fusaro / DSM 804
-
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanosarcina barkeri
-
DSM 804, gene mtaA
-
Methanosarcina barkeri Fusaro / DSM 804
-
DSM 804, gene mtaA
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.01
-
purified recombinant mutant C239A MtaA, pH 7.0, 37°C
Methanosarcina barkeri
0.02
-
purified recombinant mutant H237A MtaA, pH 7.0, 37°C
Methanosarcina barkeri
0.3
-
purified recombinant wild-type MtaA, pH 7.0, 37°C
Methanosarcina barkeri
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
-
717563
Methanosarcina barkeri
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
-
-
r
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
-
717563
Methanosarcina barkeri Fusaro, DSM 804
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
-
-
r
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
-
717563
Methanosarcina barkeri Fusaro / DSM 804
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
-
-
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Methanosarcina barkeri
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Methanosarcina barkeri
Cloned(Commentary) (protein specific)
Commentary
Organism
gene mtaA, sequence comparison, expression of His-tagged MtaA in Escherichia coli strain M15
Methanosarcina barkeri
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C239A
site-directed mutagenesis, mutation of the residue involved in zinc coordination in MtaA results in reduced zinc content and reduced activity compared to the wild-type enzyme
Methanosarcina barkeri
H237A
site-directed mutagenesis, mutation of the residue involved in zinc coordination in MtaA results in reduced zinc content and reduced activity compared to the wild-type enzyme
Methanosarcina barkeri
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
dependent on, wild-type MtaA has a zinc content of 0.91 mol/mol, binding structure, overview
Methanosarcina barkeri
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
Methanosarcina barkeri
-
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
-
r
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
Methanosarcina barkeri Fusaro, DSM 804
-
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
-
r
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
Methanosarcina barkeri Fusaro / DSM 804
-
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
-
r
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.01
-
purified recombinant mutant C239A MtaA, pH 7.0, 37°C
Methanosarcina barkeri
0.02
-
purified recombinant mutant H237A MtaA, pH 7.0, 37°C
Methanosarcina barkeri
0.3
-
purified recombinant wild-type MtaA, pH 7.0, 37°C
Methanosarcina barkeri
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
-
717563
Methanosarcina barkeri
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
-
-
r
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
-
717563
Methanosarcina barkeri Fusaro, DSM 804
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
-
-
r
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
-
717563
Methanosarcina barkeri Fusaro / DSM 804
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Methanosarcina barkeri
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Methanosarcina barkeri
Other publictions for EC 2.1.1.246
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
698630
Opulencia
Physiology and posttranscripti ...
Methanosarcina acetivorans
J. Bacteriol.
191
6928-6935
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
2
3
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
3
-
-
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
681980
Pritchett
Genetic, physiological and bio ...
Methanosarcina acetivorans
Mol. Microbiol.
56
1183-1194
2005
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
717563
Krüer
The role of zinc in the methyl ...
Methanosarcina barkeri, Methanosarcina barkeri Fusaro / DSM 804
Eur. J. Biochem.
269
2117-2123
2002
-
-
1
-
2
-
-
-
-
1
-
3
-
12
-
-
-
-
-
-
3
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
3
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
717609
Ding
Genomic and proteomic analyses ...
Methanosarcina thermophila, Methanosarcina thermophila TM-1
FEMS Microbiol. Lett.
215
127-132
2002
-
-
-
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
717562
Sauer
Methyl-coenzyme M formation in ...
Methanosarcina barkeri
Eur. J. Biochem.
267
2498-2504
2000
-
-
1
-
-
-
2
-
-
3
1
1
-
1
-
-
1
1
-
-
1
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
3
1
1
-
-
-
1
-
-
1
-
1
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
717561
Sauer
Methanol:coenzyme M methyltran ...
Methanosarcina barkeri
Eur. J. Biochem.
261
674-681
1999
3
-
1
-
-
-
1
1
-
2
-
1
-
2
-
-
1
-
-
-
2
-
2
-
1
-
-
-
1
-
-
-
-
-
-
3
-
1
-
-
-
-
-
1
-
1
-
2
-
1
-
-
-
1
-
-
2
-
2
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
717559
Sauer
Methanol:coenzyme M methyltran ...
Methanosarcina barkeri
Eur. J. Biochem.
253
698-705
1998
1
-
1
-
-
-
-
-
-
1
-
1
-
4
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
485665
Sauer
Methanol:coenzyme M methyltran ...
Methanosarcina barkeri
Eur. J. Biochem.
249
280-285
1997
-
-
-
-
-
-
-
-
-
1
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
717557
Harms
Methylcobalamin:coenzyme M met ...
Methanosarcina barkeri, Methanosarcina barkeri DSM 804
Eur. J. Biochem.
235
653-659
1996
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
1
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
1
-
-
-
1
-
-
1
-
-
717753
LeClerc
Methylcobamide:coenzyme M meth ...
Methanosarcina barkeri
J. Biol. Chem.
271
18725-18731
1996
-
-
1
-
-
-
-
1
-
1
2
2
-
3
-
-
-
-
-
1
4
-
4
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
2
2
-
-
-
-
-
1
4
-
4
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-