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Literature summary for 2.1.1.246 extracted from

  • Sauer, K.; Thauer, R.K.
    Methanol:coenzyme M methyltransferase from Methanosarcina barkeri - identification of the active-site histidine in the corrinoid-harboring subunit MtaC by site-directed mutagenesis (1998), Eur. J. Biochem., 253, 698-705.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Ti(III) citrate
-
Methanosarcina barkeri

Cloned(Commentary)

Cloned (Comment) Organism
genes mtaA and mtaB, expression of N-terminally His-tagged isozymes MtaA and MtbA in Escherichia coli strain M15 Methanosarcina barkeri

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M Methanosarcina barkeri
-
methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
?

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged isozymes MtaA and MtbA from Escherichia coli strain M15 by nickel affinity chromatography Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M
-
Methanosarcina barkeri methyl-CoM + a [Co(I) methanol-specific corrinoid protein]
-
?
additional information recombinant MtaA catalyzes the formation of methyl-coenzyme M from free CH3-cob(III)alamin and coenzyme M at specific rates comparable to those predicted for MtaA-catalyzed MtaC demethylation Methanosarcina barkeri ?
-
?

Synonyms

Synonyms Comment Organism
methanol:coenzyme M methyltransferase
-
Methanosarcina barkeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Methanosarcina barkeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Methanosarcina barkeri

General Information

General Information Comment Organism
evolution Methanosarcina barkeri contains an isoenzyme of MtaA designated MtbA. The isoenzyme reacts with MtaC with only 2.5% of the activity of MtaA Methanosarcina barkeri
metabolism the enzyme system catalyzing the formation of methyl-coenzyme M from methanol and coenzyme M in Methanosarcina barkeri is composed of the three different polypeptides MtaA, MtaB and MtaC of which MtaC harbors a corrinoid prosthetic group Methanosarcina barkeri
additional information the enzyme system catalyzing the formation of methyl-coenzyme M from methanol and coenzyme M in Methanosarcina barkeri is composed of the three different polypeptides MtaA, MtaB and MtaC of which MtaC harbors a corrinoid prosthetic group Methanosarcina barkeri