Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ti(III) citrate | - |
Methanosarcina barkeri |
Cloned (Comment) | Organism |
---|---|
genes mtaA and mtaB, expression of N-terminally His-tagged isozymes MtaA and MtbA in Escherichia coli strain M15 | Methanosarcina barkeri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required | Methanosarcina barkeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M | Methanosarcina barkeri | - |
methyl-CoM + a [Co(I) methanol-specific corrinoid protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged isozymes MtaA and MtbA from Escherichia coli strain M15 by nickel affinity chromatography | Methanosarcina barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M | - |
Methanosarcina barkeri | methyl-CoM + a [Co(I) methanol-specific corrinoid protein] | - |
? | |
additional information | recombinant MtaA catalyzes the formation of methyl-coenzyme M from free CH3-cob(III)alamin and coenzyme M at specific rates comparable to those predicted for MtaA-catalyzed MtaC demethylation | Methanosarcina barkeri | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methanol:coenzyme M methyltransferase | - |
Methanosarcina barkeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanosarcina barkeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Methanosarcina barkeri |
General Information | Comment | Organism |
---|---|---|
evolution | Methanosarcina barkeri contains an isoenzyme of MtaA designated MtbA. The isoenzyme reacts with MtaC with only 2.5% of the activity of MtaA | Methanosarcina barkeri |
metabolism | the enzyme system catalyzing the formation of methyl-coenzyme M from methanol and coenzyme M in Methanosarcina barkeri is composed of the three different polypeptides MtaA, MtaB and MtaC of which MtaC harbors a corrinoid prosthetic group | Methanosarcina barkeri |
additional information | the enzyme system catalyzing the formation of methyl-coenzyme M from methanol and coenzyme M in Methanosarcina barkeri is composed of the three different polypeptides MtaA, MtaB and MtaC of which MtaC harbors a corrinoid prosthetic group | Methanosarcina barkeri |