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Literature summary for 2.1.1.246 extracted from

  • Harms, U.; Thauer, R.
    Methylcobalamin:coenzyme M methyltransferase isoenzymes MtaA and MtbA from Methanosarcina barkeri: Cloning, sequencing and differential transcription of the encoding genes, and functional overexpression of the mtaA gene in Escherichia coli (1996), Eur. J. Biochem., 235, 653-659.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
gene mtaA, DNA and amino acid sequence determination and analysis, gene mtaA is monocislronically transcribed, transcription of the gene is positively controlled by the growth substrate rnethanol, overexpression of the His6-tagged enzyme in Escherichia coli strain M15 Methanosarcina barkeri

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri
-
 Fusaro, gene mtaA
-
Methanosarcina barkeri DSM 804
-
 Fusaro, gene mtaA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged MtaA from Escherichia coli strain M15 to homogeneity by nickel affinity and anion exchange chromatography Methanosarcina barkeri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.5
-
 purified recombinant enzyme, pH 7.2, 37°C Methanosarcina barkeri

Synonyms

Synonyms Comment Organism
methylcobalamin:coenzyme M methyltransferase
-
 Methanosarcina barkeri
mtaA
-
 Methanosarcina barkeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Methanosarcina barkeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Methanosarcina barkeri

Expression

Organism Comment Expression
Methanosarcina barkeri mtaA is induced by growth on methanol up