Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.244 extracted from

  • Tooley, C.E.; Petkowski, J.J.; Muratore-Schroeder, T.L.; Balsbaugh, J.L.; Shabanowitz, J.; Sabat, M.; Minor, W.; Hunt, D.F.; Macara, I.G.
    NRMT is an alpha-N-methyltransferase that methylates RCC1 and retinoblastoma protein (2010), Nature, 466, 1125-1128.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
N-terminally tagged FLAG-NRMT overexpression in HEK 293LT cell nuceli, that show 3fold increased RCC1 alpha-N-methyltransferase activity Homo sapiens

Protein Variants

Protein Variants Comment Organism
D168X site-directed mutagenesis, the mutation has no effect on methylation Homo sapiens
D178A/D181A site-directed mutagenesis, mutating the residues Asp178 and Asp181 at the lip of the active site to Ala decreases enzyme activity, which is further decreased by reverse-charge mutagenesis to Lys Homo sapiens
additional information depleting NRMT in 293LT cells, using lentivirus, significantly decreases methylation of endogenous RCC1, while not affecting overall RCC1 level, depleting NRMT in HeLa cells using short interfering RNAs causing the same effects, RCC1 methylation is rescued by expression of murine NRMT-FLAG, which is not targeted by the human shRNA Homo sapiens
Q169K site-directed mutagenesis, inactive mutant Homo sapiens
S183K site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
Homo sapiens 5634
-
soluble
-
Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + Ran guanine nucleotide-exchange factor RCC1 Homo sapiens NRMT is the predominant alpha-N-methyltransferase for RCC1 S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + retinoblastoma protein Homo sapiens
-
S-adenosyl-L-homocysteine + ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
gene METTL11a/C9orf32/Ad-003
-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme substrates have a unique N-terminal motif, Met-(Ala/Pro/Ser)-Pro-Lys. The initiating Met is cleaved, and the exposed alpha-amino group is mono-, di-, or trimethylated Homo sapiens ?
-
?
S-adenosyl-L-methionine + Ran guanine nucleotide-exchange factor RCC1 NRMT is the predominant alpha-N-methyltransferase for RCC1 Homo sapiens S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + Ran guanine nucleotide-exchange factor RCC1 substrate docking and mutational analysis of RCC1 defining the NRMT recognition sequence, the first 3 residues Ser-Pro-Lys interact with NRMT, overview Homo sapiens S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + retinoblastoma protein
-
Homo sapiens S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + SET/TAF-I/PHAPII only the SETalpha splicing variant is a substrate for NRMT, since it begins with the NRMT consensus in contrast to the beta splicing variant Homo sapiens S-adenosyl-L-homocysteine + ?
-
?

Subunits

Subunits Comment Organism
More NRMT lacks a SET domain but possesses a Rossman-like alpha/beta fold Homo sapiens

Synonyms

Synonyms Comment Organism
alpha-N-methyltransferase
-
Homo sapiens
METTL11a/C9orf32/Ad-003
-
Homo sapiens
N-terminal RCC1 methyltransferase
-
Homo sapiens
NRMT
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Homo sapiens

General Information

General Information Comment Organism
evolution METTL11a, i.e. NRMT, encodes a 25 kDa protein in the methyltransferase 11 family, most members of which methylate metabolites or other small molecules. alpha-N-methyltransferase is a conserved member of a superfamily of non-SET domain enzymes Homo sapiens
malfunction methylation-defective mutants of RCC1 have reduced affinity for DNA and cause mitotic defects, and non-methylatable mutants of RCC1 are defective in chromatin association, and their expression in a wild-type background produces supernumerary spindle poles and missegregation of mitotic chromosomes, most likely due to the disruption of the Ran gradient Homo sapiens
physiological function importance of alpha-N-methylation for normal bipolar spindle formation and chromosome segregation. Function of the alpha-N-methylation is not solely to stabilize chromatin associations, but may have a more general role in the regulation of electrostatic interactions Homo sapiens