Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.244 extracted from

  • Webb, K.J.; Lipson, R.S.; Al-Hadid, Q.; Whitelegge, J.P.; Clarke, S.G.
    Identification of protein N-terminal methyltransferases in yeast and humans (2010), Biochemistry, 49, 5225-5235.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene METTL11A Mus musculus
gene METTL11A, cloning of the His-tagged human enzyme using vector pET-100/DTOPO that carries a His6 tag in the linker region MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDHPFT, which is incorporated before the initiator methionine residue of the cloned protein, and expression in Escherichia coli strain BL21(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information the intact dimethylated Rpl12ab species isolated from the YBR261C/tae1 deletion strain is fragmented, and a b20 ion is detected with the addition of one methyl group, suggesting partial methylation of lysine 3. Neither of these species is dimethylated at the N-terminal residue. Rps25a/Rps25b isolated from the YBR261C/tae1 deletion strain is 28 Da less than the wild type, and the fragmentation data indicate no methylation is present Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Mus musculus 5829
-
cytosol
-
Saccharomyces cerevisiae 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae the N-terminal protein methyltransferase catalyzes the modification of two ribosomal protein substrates, Rpl12ab and Rps25a/Rps25b. The yeast RPS25A and RPS25B genes and differ only at a single amino acid residue ?
-
?
S-adenosyl-L-methionine + Rpl12ab Saccharomyces cerevisiae methylation of Rpl12ab at the N-terminal proline residue ?
-
?
S-adenosyl-L-methionine + Rps25a Saccharomyces cerevisiae
-
?
-
?
S-adenosyl-L-methionine + Rps25b Saccharomyces cerevisiae
-
?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9BV86 gene METTL11A
-
Mus musculus
-
genes METTL11A and METTL11B
-
Saccharomyces cerevisiae
-
strains BY4742 and BY4741 and the YBR261C/tae1 deletion strain in each background, gene YBR261C/TAE1
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially from heart cell cytosol Mus musculus
recombinant His-tagged METTL11A from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
heart high enzyme expression levels Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the N-terminal protein methyltransferase catalyzes the modification of two ribosomal protein substrates, Rpl12ab and Rps25a/Rps25b. The yeast RPS25A and RPS25B genes and differ only at a single amino acid residue Saccharomyces cerevisiae ?
-
?
additional information the methyltransferases specifically recognizes the N-terminal X-Pro-Lys sequence motif. Localization of methylation sites by top down mass spectrometry using collisionally activated dissociation or electron capture dissociation. The enzyme can also recognize species with N-terminal alanine and serine residues in addition to those with proline residues, but the proline residue in position 1 is a preferred substrate Homo sapiens ?
-
?
additional information the methyltransferases specifically recognizes the N-terminal X-Pro-Lys sequence motif. Localization of methylation sites by top down mass spectrometry using collisionally activated dissociation or electron capture dissociation. The yeast enzyme can also recognize species with N-terminal alanine and serine residues in addition to those with proline residues, although to a lesser extent, the proline residue in position 1 is a preferred substrate Saccharomyces cerevisiae ?
-
?
S-adenosyl-L-methionine + APKQQLSKY synthetic peptide, modified Rps25a/Rps25b-derived peptide Saccharomyces cerevisiae ?
-
?
S-adenosyl-L-methionine + APKQQLSKY synthetic peptide, modified yeast protein Rps25a/Rps25b-derived peptide Mus musculus ?
-
?
S-adenosyl-L-methionine + APKQQLSKY synthetic peptide, modified yeast protein Rps25a/Rps25b-derived peptide Homo sapiens ?
-
?
S-adenosyl-L-methionine + PPKQQLSKY synthetic peptide, Rps25a/Rps25b-derived peptide Saccharomyces cerevisiae ?
-
?
S-adenosyl-L-methionine + PPKQQLSKY synthetic peptide, yeast protein Rps25a/Rps25b-derived peptide Mus musculus ?
-
?
S-adenosyl-L-methionine + PPKQQLSKY synthetic peptide, yeast protein Rps25a/Rps25b-derived peptide Homo sapiens ?
-
?
S-adenosyl-L-methionine + Rpl12ab methylation of Rpl12ab at the N-terminal proline residue Saccharomyces cerevisiae ?
-
?
S-adenosyl-L-methionine + Rps25a
-
Saccharomyces cerevisiae ?
-
?
S-adenosyl-L-methionine + Rps25a Rps25a and Rps25b differ only at position 104, a threonine residue is present in the former and an alanine residue in the latter Saccharomyces cerevisiae ?
-
?
S-adenosyl-L-methionine + Rps25b
-
Saccharomyces cerevisiae ?
-
?
S-adenosyl-L-methionine + Rps25b Rps25a and Rps25b differ only at position 104, a threonine residue is present in the former and an alanine residue in the latter Saccharomyces cerevisiae ?
-
?
S-adenosyl-L-methionine + SPKQQLSKY synthetic peptide, modified Rps25a/Rps25b-derived peptide Saccharomyces cerevisiae ?
-
?
S-adenosyl-L-methionine + SPKQQLSKY synthetic peptide, modified yeast protein Rps25a/Rps25b-derived peptide Mus musculus ?
-
?
S-adenosyl-L-methionine + SPKQQLSKY synthetic peptide, modified yeast protein Rps25a/Rps25b-derived peptide Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
More the enzyme is a seven beta-strand methyltransferase Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
METT11B
-
Mus musculus
METTL11A
-
Mus musculus
METTL11A
-
Homo sapiens
Ntm1
-
Saccharomyces cerevisiae
NTMT1
-
Homo sapiens
YBR261C/Tae1
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Mus musculus
22
-
assay at room temperature Saccharomyces cerevisiae
22
-
assay at room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Mus musculus
7
-
assay at Saccharomyces cerevisiae
7
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Mus musculus
S-adenosyl-L-methionine
-
Saccharomyces cerevisiae
S-adenosyl-L-methionine
-
Homo sapiens

General Information

General Information Comment Organism
malfunction loss of the Saccharomyces cerevisiae ORF YBR261c/TAE results in the loss of the N-terminal methylation of both Rpl12ab and Rps25a/Rps25b Saccharomyces cerevisiae
physiological function protein X-Pro-Lys N-terminal methylation reactions catalyzed by the enzyme may be widespread in nature Homo sapiens
physiological function the N-terminal protein methyltransferase catalyzes the modification of two ribosomal protein substrates, Rpl12ab and Rps25a/Rps25b, the YBR261C/TAE1 product is necessary for the formation of the dimethylproline residue in each of these ribosomal proteins. Protein X-Pro-Lys N-terminal methylation reactions catalyzed by the enzyme may be widespread in nature Saccharomyces cerevisiae