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Literature summary for 2.1.1.240 extracted from

  • Jeong, Y.J.; An, C.H.; Woo, S.G.; Jeong, H.J.; Kim, Y.M.; Park, S.J.; Yoon, B.D.; Kim, C.Y.
    Production of pinostilbene compounds by the expression of resveratrol O-methyltransferase genes in Escherichia coli (2014), Enzyme Microb. Technol., 54, 8-14.
    View publication on PubMed
Search for more literature for 2.1.1.240

Cloned(Commentary)

Cloned (Comment) Organism
gene SbROMT3syn, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, subcloning in Escherichia coli strain DH5alpha Sorghum bicolor
gene VrROMTsyn, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, subcloning in Escherichia coli strain DH5alpha. Recombinantly expressed enzyme from grape produces very small amounts of methylated resveratrol derivatives in Escherichia coli Vitis riparia

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Sorghum bicolor
Mg2+ required Vitis riparia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene Sorghum bicolor i.e. pinostilbene S-adenosyl-L-homocysteine + pterostilbene
-
 ?
S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene Vitis riparia i.e. pinostilbene S-adenosyl-L-homocysteine + pterostilbene
-
 ?
S-adenosyl-L-methionine + trans-resveratrol Sorghum bicolor
-
 S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene
-
 ?
S-adenosyl-L-methionine + trans-resveratrol Vitis riparia
-
 S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene
-
 ?

Organism

Organism UniProt Comment Textmining
Sorghum bicolor A8QW53 gene SbROMT3syn
-
Vitis riparia K7XQ68 gene VrROMTsyn
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene i.e. pinostilbene Sorghum bicolor S-adenosyl-L-homocysteine + pterostilbene
-
 ?
S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene i.e. pinostilbene Vitis riparia S-adenosyl-L-homocysteine + pterostilbene
-
 ?
S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene i.e. pinostilbene, minor reaction, recombinant SbROMT3syn produces pinostilbene as the major product besides a very small amount of pterostilbene Sorghum bicolor S-adenosyl-L-homocysteine + pterostilbene
-
 ?
S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene i.e. pinostilbene, minor reaction, recombinant SbROMT3syn produces pinostilbene as the major product besides a very small amount of pterostilbene Vitis riparia S-adenosyl-L-homocysteine + pterostilbene
-
 ?
S-adenosyl-L-methionine + trans-resveratrol
-
 Sorghum bicolor S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene
-
 ?
S-adenosyl-L-methionine + trans-resveratrol
-
 Vitis riparia S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene
-
 ?
S-adenosyl-L-methionine + trans-resveratrol major reaction step, recombinant SbROMT3syn produces pinostilbene as the major product besides a very small amount of pterostilbene Sorghum bicolor S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene
-
 ?
S-adenosyl-L-methionine + trans-resveratrol major reaction step, recombinant SbROMT3syn produces pinostilbene as the major product besides a very small amount of pterostilbene Vitis riparia S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene
-
 ?

Synonyms

Synonyms Comment Organism
resveratrol O-methyltransferase
-
 Sorghum bicolor
resveratrol O-methyltransferase
-
 Vitis riparia
ROMT
-
 Sorghum bicolor
ROMT
-
 Vitis riparia
SbROMT3syn protein
-
 Sorghum bicolor
VrROMTsyn protein
-
 Vitis riparia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Sorghum bicolor
30
-
 assay at Vitis riparia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Sorghum bicolor
7.5
-
 assay at Vitis riparia

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Sorghum bicolor
S-adenosyl-L-methionine
-
 Vitis riparia

General Information

General Information Comment Organism
metabolism biosynthetic pathway of stilbene compound production from phenylalanine involving the enzyme, overview Sorghum bicolor
metabolism biosynthetic pathway of stilbene compound production from phenylalanine involving the enzyme, overview Vitis riparia