Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.239 extracted from

  • Rodríguez, L.; Rodríguez, D.; Olano, C.; Brana, A.F.; Mendez, C.; Salas, J.A.
    Functional analysis of OleY L-oleandrosyl 3-O-methyltransferase of the oleandomycin biosynthetic pathway in Streptomyces antibioticus (2001), J. Bacteriol., 183, 5358-5363.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.239

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli as inclusion bodies and in Streptomyces lividans or Streptomyces albus as soluble protein Streptomyces antibioticus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 0.1 mM, strong inhibition Streptomyces antibioticus
EDTA 1 mM, strong inhibition Streptomyces antibioticus
Hg2+ 0.1 mM, strong inhibition Streptomyces antibioticus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 0.1 mM, 11fold stimulation of activity Streptomyces antibioticus
Fe2+ 0.1 mM, 1.5fold stimulation of activity Streptomyces antibioticus
Mg2+ 0.1 mM, 3.5fold stimulation of activity Streptomyces antibioticus
Mn2+ 0.1 mM, 7fold stimulation of activity Streptomyces antibioticus
Zn2+ 0.1 mM, 5fold stimulation of activity Streptomyces antibioticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 2 * 43000, SDS-PAGE Streptomyces antibioticus
87000
-
 gel filtration Streptomyces antibioticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + L-olivosyl-erythronolide B Streptomyces antibioticus the enzyme is involved in biosynthesis of the macrolide antibiotic oleandomycin S-adenosyl-L-homocysteine + L-oleandrosyl-erythronolide B
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces antibioticus O87833
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces antibioticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00000256
-
 pH 8.0, 30°C Streptomyces antibioticus

Storage Stability

Storage Stability Organism
-70°C, enzyme keeps most of the activity after 30 days Streptomyces antibioticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no bioconversion is observed when other glycosylated macrolide antibiotics (spiramycin, niddamycin, carbomycin) are added to cultures of Streptomyces albus NAG2(pLR14b-4). These three macrolides contain L-mycarose moieties with a free 3-hydroxy group but are not methylated by OleY Streptomyces antibioticus ?
-
 ?
S-adenosyl-L-methionine + L-mycarosyl-erythronolide B poor substrate Streptomyces antibioticus S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + L-olivosyl-erythronolide B
-
 Streptomyces antibioticus S-adenosyl-L-homocysteine + L-oleandrosyl-erythronolide B
-
 ?
S-adenosyl-L-methionine + L-olivosyl-erythronolide B the enzyme is involved in biosynthesis of the macrolide antibiotic oleandomycin Streptomyces antibioticus S-adenosyl-L-homocysteine + L-oleandrosyl-erythronolide B
-
 ?
S-adenosyl-L-methionine + L-rhamnosyl-erythronolide B
-
 Streptomyces antibioticus S-adenosyl-L-homocysteine + ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 43000, SDS-PAGE Streptomyces antibioticus

Synonyms

Synonyms Comment Organism
OleY
-
 Streptomyces antibioticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Streptomyces antibioticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.6
-
 Streptomyces antibioticus
8
-
 assay at Streptomyces antibioticus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.8 pH 6.5: about 60% of maximal activity, pH 7.8: about 60% of maximal activity Streptomyces antibioticus