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Literature summary for 2.1.1.237 extracted from
- Functional analysis of MycE and MycF, two O-methyltransferases involved in the biosynthesis of mycinamicin macrolide antibiotics (2009), Chembiochem, 10, 1297-1301.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the antibacterial activities of some mycinamicin products against Staphylococcus aureus are higher than those of clinical macrolide antibiotics erythromycin and leucomycin | Micromonospora griseorubida |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| MycF is cloned into pET28b for overexpression in Escherichia coli BL21 (DE3) | Micromonospora griseorubida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | addition of 2 mM of EDTA fails to abrogate activity, suggesting that a metal ion might be dispensible in the O-methyltransferase reaction catalysed by MycF | Micromonospora griseorubida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0307 | - |
mycinamicin III | pH 7.4, 30°C | Micromonospora griseorubida |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activation is about 90% of the activation obtained by Mg2+ | Micromonospora griseorubida | |
| Fe2+ | activation is about 70% of the activation obtained by Mg2+ | Micromonospora griseorubida | |
| Fe2+ | activation is about 90% of the activation obtained by Mg2+ | Micromonospora griseorubida | |
| Mg2+ | optimal activity in presence of 10 mM MgCl2 | Micromonospora griseorubida | |
| Mn2+ | activation is about equal to the activation obtained by Mg2+ | Micromonospora griseorubida | |
| Zn2+ | activation is about equal to the activation obtained by Mg2+ | Micromonospora griseorubida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28600 | - |
28600, SDS-PAGE | Micromonospora griseorubida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + mycinamicin III | Micromonospora griseorubida | the enzyme is involved in the biosynthesis of mycinamicin macrolide antibiotics | S-adenosyl-L-homocysteine + mycinamicin IV | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Micromonospora griseorubida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminal His6-tagged protein, purified by one-step Ni-NTA agarose chromatography | Micromonospora griseorubida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + mycinamicin III | the enzyme is involved in the biosynthesis of mycinamicin macrolide antibiotics | Micromonospora griseorubida | S-adenosyl-L-homocysteine + mycinamicin IV | - |
? | |
| S-adenosyl-L-methionine + mycinamicin III | methylation of the C3''-OH of javose. Mycf possesses high substrate specificity. MycE is methylating mycinamycin VI | Micromonospora griseorubida | S-adenosyl-L-homocysteine + mycinamicin IV | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | 28600, SDS-PAGE | Micromonospora griseorubida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MycF | - |
Micromonospora griseorubida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Micromonospora griseorubida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.225 | - |
mycinamicin III | pH 7.4, 30°C | Micromonospora griseorubida | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Micromonospora griseorubida |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in the biosynthesis of mycinamicin macrolide antibiotics. Mycinamicins represent a family of macrolide antibiotics with more than twenty members produced by the rare actinomycete Micromonospora griseorubida | Micromonospora griseorubida |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.3 | - |
mycinamicin III | pH 7.4, 30°C | Micromonospora griseorubida | |
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