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show all sequences of 2.1.1.236

Characterization of the TDP-D-ravidosamine biosynthetic pathway: one-pot enzymatic synthesis of TDP-D-ravidosamine from thymidine-5-phosphate and glucose-1-phosphate

Kharel, M.K.; Lian, H.; Rohr, J.; Org. Biomol. Chem. 9, 1799-1808 (2011)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
synthesis of TDP-D-ravidosamine, necessary for future in vitro glycosylation assays. TDP-D-ravidosamine is the anticipated sugar donor substrate of RavGT (the glycosyltransferase that links D-ravidosamine to the polyketide derived backbone defuco-gilvocarcin V). Defuco-gilvocarcin V exhibits superior anticancer/antibacterial activities
Streptomyces ravidus
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Streptomyces ravidus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31060
-
2 * 31060, SDS-PAGE
Streptomyces ravidus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces ravidus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Streptomyces ravidus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 S-adenosyl-L-methionine + dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose
RavNMT catalyzes the transfer of methyl groups from S-adenosyl-L-methionine to the amino nitrogen of in a sequential manner
716410
Streptomyces ravidus
2 S-adenosyl-L-homocysteine + TDP-3-dimethylamino-3,6-dideoxy-alpha-D-galactopyranose
TDP-3-dimethylamino-3,6-dideoxy-alpha-D-galactopyranose i.e. TDP-D-ravidosamine
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 31060, SDS-PAGE
Streptomyces ravidus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Streptomyces ravidus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Streptomyces ravidus
Application (protein specific)
Application
Commentary
Organism
synthesis
synthesis of TDP-D-ravidosamine, necessary for future in vitro glycosylation assays. TDP-D-ravidosamine is the anticipated sugar donor substrate of RavGT (the glycosyltransferase that links D-ravidosamine to the polyketide derived backbone defuco-gilvocarcin V). Defuco-gilvocarcin V exhibits superior anticancer/antibacterial activities
Streptomyces ravidus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Streptomyces ravidus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31060
-
2 * 31060, SDS-PAGE
Streptomyces ravidus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces ravidus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 S-adenosyl-L-methionine + dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose
RavNMT catalyzes the transfer of methyl groups from S-adenosyl-L-methionine to the amino nitrogen of in a sequential manner
716410
Streptomyces ravidus
2 S-adenosyl-L-homocysteine + TDP-3-dimethylamino-3,6-dideoxy-alpha-D-galactopyranose
TDP-3-dimethylamino-3,6-dideoxy-alpha-D-galactopyranose i.e. TDP-D-ravidosamine
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 31060, SDS-PAGE
Streptomyces ravidus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Streptomyces ravidus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Streptomyces ravidus
Other publictions for EC 2.1.1.236
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735676
Thoden
Production of a novel N-monome ...
Streptomyces fradiae
Biochemistry
53
1105-1107
2014
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716410
Kharel
Characterization of the TDP-D- ...
Streptomyces ravidus
Org. Biomol. Chem.
9
1799-1808
2011
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