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Literature summary for 2.1.1.233 extracted from

  • Creighton, M.T.; Kolton, A.; Kataya, A.R.A.; Maple-Grodem, J.; Averkina, I.O.; Heidari, B.; Lillo, C.
    Methylation of protein phosphatase 2A - influence of regulators and environmental stress factors (2017), Plant Cell Environ., 40, 2347-2358 .
    View publication on PubMed
Search for more literature for 2.1.1.233

Protein Variants

Protein Variants Comment Organism
additional information generation of a knockout lcmt1 mutant sbi1, phenotype, overview. PP2A-C methylation status in wild-type and lcmt1 mutant seedlings under control conditions Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Arabidopsis thaliana
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + [phosphatase 2A-C protein]-leucine Arabidopsis thaliana
-
 S-adenosyl-L-homocysteine + [phosphatase 2A-C protein]-leucine methyl ester
-
 ?
S-adenosyl-L-methionine + [phosphatase 2A-C protein]-leucine Arabidopsis thaliana Col-0
-
 S-adenosyl-L-homocysteine + [phosphatase 2A-C protein]-leucine methyl ester
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8VY08
-
-
Arabidopsis thaliana Col-0 Q8VY08
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information enzyme expression analysis in wild-type and mutant plant organs, overview Arabidopsis thaliana
-
root
-
 Arabidopsis thaliana
-
rosette leaf
-
 Arabidopsis thaliana
-
seedling
-
 Arabidopsis thaliana
-
shoot
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + [phosphatase 2A-C protein]-leucine
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + [phosphatase 2A-C protein]-leucine methyl ester
-
 ?
S-adenosyl-L-methionine + [phosphatase 2A-C protein]-leucine
-
 Arabidopsis thaliana Col-0 S-adenosyl-L-homocysteine + [phosphatase 2A-C protein]-leucine methyl ester
-
 ?

Synonyms

Synonyms Comment Organism
LCMT-1
-
 Arabidopsis thaliana
leucine carboxyl methyltransferase 1 homolog UniProt Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction the lcmt1 knockout mutant possesses essentially only unmethylated PP2A-C, has less dense rosettes, and earlier flowering compared to wild-type plants. Approximately 200 overlapping genes are 2fold upregulated, and 200 overlapping genes are 2fold downregulated in both lcmt1 and pme1 knockout mutants compared to wild-type. Differences between the 2 mutants, 97 genes are 2fold upregulated in pme1 compared with lcmt1, indicating that PME1 acts as a negative regulator for these genes. Arabidopsis thaliana plants with knocked out LCMT1 (sbi1 mutant) are void of methylated PP2A-C and have unmethylated catalytic subunit PP2A-C both in the microsomal and cytosolic fractions Arabidopsis thaliana
metabolism protein phosphatase 2A catalytic subunit (PP2A-C) has a terminal leucine subjected to methylation, a regulatory mechanism conserved from yeast to mammals and plants. Two enzymes, leucine carboxyl methyltransferase (LCMT-1) and protein phosphatase methylesterase (PME-1, EC 3.1.1.89), methylate and demethylate PP2A-C, respectively, physiological importance of these posttranslational modifications. Regulation, overview. In addition to catalyzing demethylation of PP2A-C, PME1 has a function in stabilizing the PP2A complex, keeping part of the PP2AC pool in an inactive state Arabidopsis thaliana
physiological function protein phosphatase 2A catalytic subunit (PP2A-C) has a terminal leucine subjected to methylation, a regulatory mechanism conserved from yeast to mammals and plants. Two enzymes, leucine carboxyl methyltransferase (LCMT-1) and protein phosphatase methylesterase (PME-1, EC 3.1.1.89), methylate and demethylate PP2A-C, respectively, physiological importance of these posttranslational modifications. Active LCMT1 methylates all PP2A-C in microsomes Arabidopsis thaliana