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Literature summary for 2.1.1.233 extracted from

  • MacKay, K.B.; Tu, Y.; Young, S.G.; Clarke, S.G.
    Circumventing embryonic lethality with Lcmt1 deficiency: generation of hypomorphic Lcmt1 mice with reduced protein phosphatase 2A methyltransferase expression and defects in insulin signaling (2013), PLoS ONE, 8, e65967.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.233

Cloned(Commentary)

Cloned (Comment) Organism
gene lcmt1, DNA and amino acid sequence determination and analysis, quantitative expression analysis in wild-type and mutant mice Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information generation of hypomorphic Lcmt1 mice with reduced protein phosphatase 2A methyltransferase expression and defects in insulin signaling, circumventing embryonic lethality with Lcmt1 deficiency Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Mus musculus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 x * 38000, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus enzyme LCMT1 is a class 1 S-adenosylmethionine-dependent methyltransferase with PP2A as its only known substrate ?
-
 ?
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309 Mus musculus
-
 S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain highest expression level Mus musculus
-
heart
-
 Mus musculus
-
kidney
-
 Mus musculus
-
liver
-
 Mus musculus
-
muscle
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme LCMT1 is a class 1 S-adenosylmethionine-dependent methyltransferase with PP2A as its only known substrate Mus musculus ?
-
 ?
additional information peptides mimicking the C-terminal tail of PP2A are not substrates of LCMT1 Mus musculus ?
-
 ?
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309
-
 Mus musculus S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester
-
 ?
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309 specific for Mus musculus S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
LCMT1
-
 Mus musculus
leucine carboxyl methyltransferase 1
-
 Mus musculus
PP2A methyltransferase
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine dependent on Mus musculus

General Information

General Information Comment Organism
malfunction hypomorphic Lcmt1 mice show reduced protein phosphatase 2A methyltransferase expression due to splicing around the insertion, Lcmt1 transcript and LCMT1 protein levels are reduced but not eliminated. LCMT1 activity and methylation of protein phosphatase 2A are reduced in a coordinate fashion, suggesting that LCMT1 is the only PP2A methyltransferase. The mice exhibit an insulin-resistance phenotype. Knockout of Lcmt1 in mice is lethal during embryonic development. Male Lcmt1-/- animals display increased glucose-stimulated insulin secretion, increased insulin resistance, and decreased methylation of phosphatase 2A protein Mus musculus
physiological function protein phosphatase 2A is the major serine/threonine phosphatase in eukaryotic cells, its assembly is governed by a variety of mechanisms, one of which is carboxyl-terminal methylation of the catalytic subunit by the leucine carboxyl methyltransferase LCMT1. Protein phosphatase 2A is nearly stoichiometrically methylated in the cytosol. Role for this methyltransferase in signaling in insulin-sensitive tissues Mus musculus