BRENDA - Enzyme Database show
show all sequences of 2.1.1.233

Regulation of PP2AC carboxylmethylation and cellular localisation by inhibitory class G-protein coupled receptors in cardiomyocytes

Longman, M.; Ranieri, A.; Avkiran, M.; Snabaitis, A.; PLoS ONE 9, e86234 (2014)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
stimulation of Gi-coupled adenosine type 1 receptors with N6-cyclopentyladenosine, and of other Gi-coupled receptors such as muscarinic M2 receptors (stimulated with carbachol) and angiotensin II AT2 receptors (stimulated with CGP42112) in adult rat ventricular myocytes, specifically induces methyltransferase LCMT-1 for PP2AC carboxylmethylation at Leu309 in a concentration-dependent manner. PI3K activity is upregulated in response to adenosine type 1 receptor stimulation
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
predominantly
Rattus norvegicus
5737
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309
Rattus norvegicus
-
S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester
-
-
?
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309
Rattus norvegicus Wistar
-
S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
Q6P4Z6
-
-
Rattus norvegicus Wistar
Q6P4Z6
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cardiomyocyte
ventricular
Rattus norvegicus
-
heart
-
Rattus norvegicus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309
-
732777
Rattus norvegicus
S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester
-
-
-
?
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309
-
732777
Rattus norvegicus Wistar
S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Rattus norvegicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
stimulation of Gi-coupled adenosine type 1 receptors with N6-cyclopentyladenosine, and of other Gi-coupled receptors such as muscarinic M2 receptors (stimulated with carbachol) and angiotensin II AT2 receptors (stimulated with CGP42112) in adult rat ventricular myocytes, specifically induces methyltransferase LCMT-1 for PP2AC carboxylmethylation at Leu309 in a concentration-dependent manner. PI3K activity is upregulated in response to adenosine type 1 receptor stimulation
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
predominantly
Rattus norvegicus
5737
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309
Rattus norvegicus
-
S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester
-
-
?
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309
Rattus norvegicus Wistar
-
S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cardiomyocyte
ventricular
Rattus norvegicus
-
heart
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309
-
732777
Rattus norvegicus
S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester
-
-
-
?
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309
-
732777
Rattus norvegicus Wistar
S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
the enzyme regulates the activity and subcellular localisation of type 2A protein phosphatase, PP2A, by reversible carboxylmethylation of its C-terminal leucine 309 residue. Stimulation of adenosine type 1 receptors induces PP2AC carboxylmethylation via PI3K and alters subcellular distribution in adult rat ventricular myocytes
Rattus norvegicus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme regulates the activity and subcellular localisation of type 2A protein phosphatase, PP2A, by reversible carboxylmethylation of its C-terminal leucine 309 residue. Stimulation of adenosine type 1 receptors induces PP2AC carboxylmethylation via PI3K and alters subcellular distribution in adult rat ventricular myocytes
Rattus norvegicus
Other publictions for EC 2.1.1.233
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735900
Xia
A LCMT1-PME-1 methylation equi ...
Homo sapiens
Cell Cycle
14
1938-1947
2015
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732777
Longman
Regulation of PP2AC carboxylme ...
Rattus norvegicus, Rattus norvegicus Wistar
PLoS ONE
9
e86234
2014
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722804
DeGrande
Molecular mechanisms underlyin ...
Homo sapiens
J. Biol. Chem.
288
1032-1046
2013
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Sontag
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Homo sapiens
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Circumventing embryonic lethal ...
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Tsai
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The structural basis for tight ...
Homo sapiens
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715981
Sontag
Regulation of protein phosphat ...
Mus musculus
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2010
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1
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714919
Longin
Spatial control of protein pho ...
Homo sapiens
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68-81
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4
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Lee
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Homo sapiens, Mus musculus
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4
4
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715475
Leulliot
Structure of protein phosphata ...
Saccharomyces cerevisiae
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279
8351-8358
2004
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715450
Wei
Carboxymethylation of the PP2A ...
Saccharomyces cerevisiae
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276
1570-1577
2001
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2
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655325
Tolstykh
Carboxyl methylation regulates ...
Bos taurus
EMBO J.
19
5682-5691
2000
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1
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714142
De Baere
Purification of porcine brain ...
Homo sapiens, Sus scrofa
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16539-16547
1999
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6
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715418
Xie
Protein phosphatase 2A is reve ...
Bos taurus
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269
1981-1984
1994
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