BRENDA - Enzyme Database show
show all sequences of 2.1.1.233

Molecular mechanisms underlying cardiac protein phosphatase 2A regulation in heart

DeGrande, S.; Little, S.; Nixon, D.; Wright, P.; Snyder, J.; Dun, W.; Murphy, N.; Kilic, A.; Higgins, R.; Binkley, P.; Boyden, P.; Carnes, C.; Anderson, M.; Hund, T.; Mohler, P.; J. Biol. Chem. 288, 1032-1046 (2013)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309
Homo sapiens
-
S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Homo sapiens
-
heart
left ventricular tissue
Homo sapiens
-
myocyte
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309
-
722804
Homo sapiens
S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309
Homo sapiens
-
S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Homo sapiens
-
heart
left ventricular tissue
Homo sapiens
-
myocyte
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309
-
722804
Homo sapiens
S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
decreased phosphatase 2A protein catalytic subunit methylation is linked with decreased LCMT-1 expression causing human heart failure, overview
Homo sapiens
metabolism
past global read-outs of cellular PP2A activity more appropriately represent the collective activity of numerous individual PP2A holoenzymes, each displaying a specific subcellular localization (dictated by select PP2A regulatory subunits) as well as local specific post-translational catalytic subunit methylation and phosphorylation events that regulate local and rapid holoenzyme assembly/disassembly via leucine carboxymethyltransferase 1/phosphatase methylesterase 1 (LCMT-1/PME-1), PP2A regulation system involving nine different PP2A regulatory subunits (PPP2R2A, PPP2R2B, PPP2R3A, PPP2R4, PPP2R5A, PPP2R5B, PPP2R5C, PPP2R5D, and PPP2R5E) in the heart. LCMT-1 and PME-1 show cell-type and disease-linked expressional regulation, overview
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
malfunction
decreased phosphatase 2A protein catalytic subunit methylation is linked with decreased LCMT-1 expression causing human heart failure, overview
Homo sapiens
metabolism
past global read-outs of cellular PP2A activity more appropriately represent the collective activity of numerous individual PP2A holoenzymes, each displaying a specific subcellular localization (dictated by select PP2A regulatory subunits) as well as local specific post-translational catalytic subunit methylation and phosphorylation events that regulate local and rapid holoenzyme assembly/disassembly via leucine carboxymethyltransferase 1/phosphatase methylesterase 1 (LCMT-1/PME-1), PP2A regulation system involving nine different PP2A regulatory subunits (PPP2R2A, PPP2R2B, PPP2R3A, PPP2R4, PPP2R5A, PPP2R5B, PPP2R5C, PPP2R5D, and PPP2R5E) in the heart. LCMT-1 and PME-1 show cell-type and disease-linked expressional regulation, overview
Homo sapiens
Other publictions for EC 2.1.1.233
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735900
Xia
A LCMT1-PME-1 methylation equi ...
Homo sapiens
Cell Cycle
14
1938-1947
2015
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732777
Longman
Regulation of PP2AC carboxylme ...
Rattus norvegicus, Rattus norvegicus Wistar
PLoS ONE
9
e86234
2014
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1
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722804
DeGrande
Molecular mechanisms underlyin ...
Homo sapiens
J. Biol. Chem.
288
1032-1046
2013
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2
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736425
Sontag
Leucine carboxyl methyltransfe ...
Homo sapiens
J. Biol. Chem.
288
27396-27405
2013
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1
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1
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737114
MacKay
Circumventing embryonic lethal ...
Mus musculus
PLoS ONE
8
e65967
2013
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1
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2
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713619
Tsai
The structure of human leucine ...
Homo sapiens
Acta Crystallogr. Sect. D
67
14-24
2011
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1
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716219
Stanevich
The structural basis for tight ...
Homo sapiens
Mol. Cell.
41
331-342
2011
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1
9
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2
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715981
Sontag
Regulation of protein phosphat ...
Mus musculus
J. Neurochem.
115
1455-1465
2010
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714919
Longin
Spatial control of protein pho ...
Homo sapiens
Exp. Cell Res.
314
68-81
2008
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4
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715490
Lee
Leucine carboxyl methyltransfe ...
Homo sapiens, Mus musculus
J. Biol. Chem.
282
30974-30984
2007
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4
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715475
Leulliot
Structure of protein phosphata ...
Saccharomyces cerevisiae
J. Biol. Chem.
279
8351-8358
2004
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1
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715450
Wei
Carboxymethylation of the PP2A ...
Saccharomyces cerevisiae
J. Biol. Chem.
276
1570-1577
2001
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655325
Tolstykh
Carboxyl methylation regulates ...
Bos taurus
EMBO J.
19
5682-5691
2000
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714142
De Baere
Purification of porcine brain ...
Homo sapiens, Sus scrofa
Biochemistry
38
16539-16547
1999
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715418
Xie
Protein phosphatase 2A is reve ...
Bos taurus
J. Biol. Chem.
269
1981-1984
1994
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