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Literature summary for 2.1.1.233 extracted from

  • Stanevich, V.; Jiang, L.; Satyshur, K.; Li, Y.; Jeffrey, P.; Li, Z.; Menden, P.; Semmelhack, M.; Xing, Y.
    The structural basis for tight control of PP2A methylation and function by LCMT-1 (2011), Mol. Cell., 41, 331-342.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.233

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in isolation and in complex with phosphatase 2A protein stabilized by a cofactor mimic, hanging drop vapor diffusion method, using 17-19% (w/v) PEG2000 monomethyl ether, 150 mM triethylamine N-oxide, and 5 mM dithiothreitol Homo sapiens

Protein Variants

Protein Variants Comment Organism
C36A the mutant shows about wild type activity Homo sapiens
D303R the mutant shows less than 10% activity compared to the wild type enzyme Homo sapiens
E65R the mutant shows less than 10% activity compared to the wild type enzyme Homo sapiens
F237D the mutant shows less than 10% activity compared to the wild type enzyme Homo sapiens
K62E the mutant shows less than 10% activity compared to the wild type enzyme Homo sapiens
N244A the mutant shows less than 40% activity compared to the wild type enzyme Homo sapiens
R236D the mutant shows about 80% activity compared to the wild type enzyme Homo sapiens
R73A inactive Homo sapiens
T29V the mutant shows about 10% activity compared to the wild type enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Source 15S column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine
-
 Homo sapiens S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine methyl ester
-
 ?

Synonyms

Synonyms Comment Organism
LCMT-1
-
 Homo sapiens
leucine carboxyl methyltransferase 1
-
 Homo sapiens
PP2A-specific methyltransferase
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction a dominant-negative leucine carboxyl methyltransferase 1 mutant attenuates the cell cycle without causing cell death, likely by inhibiting uncontrolled phosphatase activity Homo sapiens
physiological function the enzyme tightly controls phosphatase 2A protein function, important for the cell cycle and cell survival Homo sapiens