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Literature summary for 2.1.1.233 extracted from
- Purification of porcine brain protein phosphatase 2A leucine carboxyl methyltransferase and cloning of the human homologue (1999), Biochemistry, 38, 16539-16547.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) pLysS cells | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
x * 38000, SDS-PAGE | Homo sapiens |
| 38305 | - |
x * 38305, calculated from amino acid sequence | Homo sapiens |
| 40000 | - |
x * 40000, SDS-PAGE | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| phenyl Sepharose column chromatography, DEAE cellulose column chromatography, Poros ion exchange column chromatography, DEAE Sephacelcolumn chromatography, and Sephadex G-75 gel filtration | Homo sapiens |
| phenyl Sepharose column chromatography, DEAE cellulose column chromatography, Poros ion exchange column chromatography, DEAE Sephacelcolumn chromatography, and Sephadex G-75 gel filtration | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0000006 | - |
crude extract, at 30°C in 20 mM Tris, 0.5 mM dithiothreitol, pH 7.4 | Sus scrofa |
| 0.0046 | - |
purified enzyme, at 30°C in 20 mM Tris, 0.5 mM dithiothreitol, pH 7.4 | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity changes are observed upon methylation of dimeric and trimeric phosphatase 2A protein | Homo sapiens | ? | - |
? |  |
| additional information | no activity changes are observed upon methylation of dimeric and trimeric phosphatase 2A protein | Sus scrofa | ? | - |
? | |
| S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine | the enzyme exclusively methylates the carboxyl group of the C-terminal leucine residue of the catalytic subunit of protein phosphatase 2A (Leu309) | Homo sapiens | S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine methyl ester | - |
? | |
| S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine | the enzyme exclusively methylates the carboxyl group of the C-terminal leucine residue of the catalytic subunit of protein phosphatase 2A (Leu309) | Sus scrofa | S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine methyl ester | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, SDS-PAGE | Homo sapiens |
| ? | x * 40000, SDS-PAGE | Sus scrofa |
| ? | x * 38305, calculated from amino acid sequence | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PP2A LCMT | - |
Homo sapiens |
| PP2A LCMT | - |
Sus scrofa |
| protein phosphatase 2A leucine carboxyl methyltransferase | - |
Homo sapiens |
| protein phosphatase 2A leucine carboxyl methyltransferase | - |
Sus scrofa |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Homo sapiens | calculated from amino acid sequence | - |
5.7 |
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