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Literature summary for 2.1.1.232 extracted from
- Naringenin 7-O-methyltransferase involved in the biosynthesis of the flavanone phytoalexin sakuranetin from rice (Oryza sativa L.) (2000), Plant Sci., 155, 213-221.
General Stability
| General Stability | Organism |
|---|---|
| after affinity chromatography, the enzyme becomes labile, losing almost all activity after 24 h storage at 4°C or at -20°C | Oryza sativa |
| enzyme preparation after gel filtration retains more than 50% of its activity after 2 weeks at -20°C | Oryza sativa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 5 mM, 60% inhibition | Oryza sativa |  |
| Ca2+ | 5 mM, 63% inhibition | Oryza sativa | |
| Cd2+ | 5 mM, complete inhibition | Oryza sativa | |
| Co2+ | 5 mM, 39% inhibition | Oryza sativa | |
| Cu2+ | 5 mM, complete inhibition | Oryza sativa | |
| EDTA | 10 mM, 31% inhibition | Oryza sativa | |
| Hg2+ | 5 mM, complete inhibition | Oryza sativa | |
| Mg2+ | 5 mM, 40% inhibition | Oryza sativa | |
| Mn2+ | 5 mM, complete inhibition | Oryza sativa | |
| Ni2+ | 5 mM, complete inhibition | Oryza sativa | |
| Zn2+ | 5 mM, complete inhibition | Oryza sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
gel filtration | Oryza sativa |
| 41000 | - |
1 * 41000, SDS-PAGE | Oryza sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + (2S)-5,7,4'-trihydroxyflavanone | Oryza sativa | i.e. (2S)-naringenin. The enzyme is involved in the biosynthesis of the flavanone phytoalexin sakuranetin. Plants possess inducible defence mechanisms against pathogen attack that include the production of phytoalexins | S-adenosyl-L-homocysteine + (2S)-5,4'-dihydroxy-7-methoxyflavanone | i.e. (2S)-sakuranetin. The product of the reaction, sakuranetin is a major rice phytoalexin accumulating both in ultraviolet irradiated and blast infected as well as in copper chloride or jasmonic acid, treated rice leaves | ? | |
| S-adenosyl-L-methionine + (2S)-5,7,4'-trihydroxyflavanone | Oryza sativa L. Hitomebore | i.e. (2S)-naringenin. The enzyme is involved in the biosynthesis of the flavanone phytoalexin sakuranetin. Plants possess inducible defence mechanisms against pathogen attack that include the production of phytoalexins | S-adenosyl-L-homocysteine + (2S)-5,4'-dihydroxy-7-methoxyflavanone | i.e. (2S)-sakuranetin. The product of the reaction, sakuranetin is a major rice phytoalexin accumulating both in ultraviolet irradiated and blast infected as well as in copper chloride or jasmonic acid, treated rice leaves | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
- |
- |
| Oryza sativa L. Hitomebore | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryza sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | UV-irradiated | Oryza sativa | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, enzyme in crude homogenates is stable for over 1 month | Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with 3,5,7,4'-tetrahydroxyflavanone (kaempferol), 5,7,4'-trihydroxyisoflavone (genistein), 7,4'-dihydroxyisoflavone (daidzein), 5,4'-dihydroxy-7-methoxyflavanone (sakuranetin), or 3,4-dihydroxycinnamic acid (caffeic acid) | Oryza sativa | ? | - |
? | |
| additional information | no activity with 3,5,7,4'-tetrahydroxyflavanone (kaempferol), 5,7,4'-trihydroxyisoflavone (genistein), 7,4'-dihydroxyisoflavone (daidzein), 5,4'-dihydroxy-7-methoxyflavanone (sakuranetin), or 3,4-dihydroxycinnamic acid (caffeic acid) | Oryza sativa L. Hitomebore | ? | - |
? | |
| S-adenosyl-L-methionine + (2S)-5,7,4'-trihydroxyflavanone | i.e. (2S)-naringenin. The enzyme is involved in the biosynthesis of the flavanone phytoalexin sakuranetin. Plants possess inducible defence mechanisms against pathogen attack that include the production of phytoalexins | Oryza sativa | S-adenosyl-L-homocysteine + (2S)-5,4'-dihydroxy-7-methoxyflavanone | i.e. (2S)-sakuranetin. The product of the reaction, sakuranetin is a major rice phytoalexin accumulating both in ultraviolet irradiated and blast infected as well as in copper chloride or jasmonic acid, treated rice leaves | ? | |
| S-adenosyl-L-methionine + (2S)-5,7,4'-trihydroxyflavanone | i.e. (2S)-naringenin | Oryza sativa | S-adenosyl-L-homocysteine + (2S)-5,4'-dihydroxy-7-methoxyflavanone | i.e. (2S)-sakuranetin | ? | |
| S-adenosyl-L-methionine + (2S)-5,7,4'-trihydroxyflavanone | i.e. (2S)-naringenin. The enzyme is involved in the biosynthesis of the flavanone phytoalexin sakuranetin. Plants possess inducible defence mechanisms against pathogen attack that include the production of phytoalexins | Oryza sativa L. Hitomebore | S-adenosyl-L-homocysteine + (2S)-5,4'-dihydroxy-7-methoxyflavanone | i.e. (2S)-sakuranetin. The product of the reaction, sakuranetin is a major rice phytoalexin accumulating both in ultraviolet irradiated and blast infected as well as in copper chloride or jasmonic acid, treated rice leaves | ? | |
| S-adenosyl-L-methionine + (2S)-5,7,4'-trihydroxyflavanone | i.e. (2S)-naringenin | Oryza sativa L. Hitomebore | S-adenosyl-L-homocysteine + (2S)-5,4'-dihydroxy-7-methoxyflavanone | i.e. (2S)-sakuranetin | ? | |
| S-adenosyl-L-methionine + 3',4',5,7-tetrahydroxyflavanone | i.e. luteolin, 148% of the activity with naringenin | Oryza sativa | S-adenosyl-L-homocysteine + 3,4',5-trihydroxy-7-methoxyflavanone | - |
? | |
| S-adenosyl-L-methionine + 3',4',5,7-tetrahydroxyflavanone | i.e. luteolin, 148% of the activity with naringenin | Oryza sativa L. Hitomebore | S-adenosyl-L-homocysteine + 3,4',5-trihydroxy-7-methoxyflavanone | - |
? | |
| S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone | i.e. apigenin, 81% of the activity with naringenin | Oryza sativa | S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone | i.e. genkwanin | ? | |
| S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone | i.e. apigenin, 81% of the activity with naringenin | Oryza sativa L. Hitomebore | S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone | i.e. genkwanin | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 41000, SDS-PAGE | Oryza sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NOMT | - |
Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | Tris-HCl buffer | Oryza sativa |
| 8 | 10 | glycine-NaOH buffer | Oryza sativa |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Oryza sativa | the enzyme is induced by UV irradiation, jasmonic acid and CuCl2 (weak) | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in the biosynthesis of the flavanone phytoalexin sakuranetin. Plants possess inducible defence mechanisms against pathogen attack that include the production of phytoalexins. The product of the reaction, sakuranetin is a major rice phytoalexin accumulating both in ultraviolet irradiated and blast infected as well as in copper chloride or jasmonic acid, treated rice leaves | Oryza sativa |
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