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Literature summary for 2.1.1.231 extracted from
- Characterization of regioselective flavonoid O-methyltransferase from the Streptomyces sp. KCTC 0041BP (2018), Enzyme Microb. Technol., 113, 29-36 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene gerMIII, GerMIII is present on the biosynthetic gene cluster of dihydrochalcomycin, a macrolide antibiotic that is produced by the Streptomyces sp. KCTC 0041BP, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Streptomyces sp. KCTC 0041BP |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | complete inhibition at 20 mM | Streptomyces sp. KCTC 0041BP |  |
| Cu2+ | complete inhibition at 20 mM | Streptomyces sp. KCTC 0041BP | |
| EDTA | - |
Streptomyces sp. KCTC 0041BP | |
| Fe2+ | complete inhibition at 20 mM | Streptomyces sp. KCTC 0041BP | |
| Mn2+ | complete inhibition at 20 mM | Streptomyces sp. KCTC 0041BP | |
| Ni2+ | complete inhibition at 20 mM | Streptomyces sp. KCTC 0041BP | |
| Pb2+ | complete inhibition at 20 mM | Streptomyces sp. KCTC 0041BP | |
| Zn2+ | complete inhibition at 20 mM | Streptomyces sp. KCTC 0041BP | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetic analysis | Streptomyces sp. KCTC 0041BP | |
| 0.00183 | - |
S-adenosyl-L-methionine | pH 7.5, 37°C | Streptomyces sp. KCTC 0041BP | |
| 0.163 | - |
quercetin | pH 7.5, 37°C | Streptomyces sp. KCTC 0041BP | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, 2fold activation at 20 mM. Mg2+ comprises an octahedral coordination environment that is suitable for the two aspartic-acid residues Asp279 and Asp308 and two water molecules | Streptomyces sp. KCTC 0041BP | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + quercetin | Streptomyces sp. KCTC 0041BP | - |
S-adenosyl-L-homocysteine + tamarixetin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces sp. KCTC 0041BP | Q331Q6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography | Streptomyces sp. KCTC 0041BP |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | among the flavonoids, quercetin is the most favorable substrate, followed by luteolin, myricetin, quercetin glucoside, and fisetin, while only a single product is formed in each case. Product identification by mass-spectrometry and NMR spectrometric analysis. Susbtrate specificity, overview. Mechanistic overview of the regiospecific modification, a double bond between the C2 and the C3 and a single-ring-appended conjugate-hydroxyl group are crucial for the favorable enzymatic conversions of the GerMIII catalysis, modeling and molecular docking. Of all of the anthraquinones that are tested, only alizarin is methylated by the GerMIII at a detectable amount, whereas the methylation of all of the other remaining compounds is not evident. The common feature among the GerMIII substrates is the existence of two neighboring hydroxyl groups in the presence of a double bond between the C2 and the C3, as found in the quercetin, luteolin, myricetin, fisetin, and quercetin glucoside. GerMIII does not methylate many substrates including the close flavonoid relative catechin, which lacks the double bond between the C2 and the C3 | Streptomyces sp. KCTC 0041BP | ? | - |
- |
|
| S-adenosyl-L-methionine + alizarin | - |
Streptomyces sp. KCTC 0041BP | S-adenosyl-L-homocysteine + methylalizarin | - |
? | |
| S-adenosyl-L-methionine + fisetin | 8% activity with fisetin compared to quercetin | Streptomyces sp. KCTC 0041BP | S-adenosyl-L-homocysteine + 4'-methoxyfisetin | - |
? | |
| S-adenosyl-L-methionine + luteolin | 67% activity with luteolin compared to quercetin | Streptomyces sp. KCTC 0041BP | S-adenosyl-L-homocysteine + 4'-methoxyluteolin | - |
? | |
| S-adenosyl-L-methionine + myricetin | 20% activity with luteolin compared to quercetin | Streptomyces sp. KCTC 0041BP | S-adenosyl-L-homocysteine + 4'-methoxymyricetin | - |
? | |
| S-adenosyl-L-methionine + quercetin | best substrate, 85% conversion at 2 mM substrate | Streptomyces sp. KCTC 0041BP | S-adenosyl-L-homocysteine + 4'-methoxyquercetin | - |
? | |
| S-adenosyl-L-methionine + quercetin | - |
Streptomyces sp. KCTC 0041BP | S-adenosyl-L-homocysteine + tamarixetin | - |
? | |
| S-adenosyl-L-methionine + quercetin 3-O-beta-D-glucoside | 16% activity with quercetin glucoside compared to quercetin | Streptomyces sp. KCTC 0041BP | S-adenosyl-L-homocysteine + 4'-methoxyquercetin 3-O-beta-D-glucoside | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44000, about, sequence calculation | Streptomyces sp. KCTC 0041BP |
| More | three-dimensional enzyme structure analysis and modelling, overview | Streptomyces sp. KCTC 0041BP |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4'OMT | - |
Streptomyces sp. KCTC 0041BP |
| GerMIII | - |
Streptomyces sp. KCTC 0041BP |
| O-methyltransferase | - |
Streptomyces sp. KCTC 0041BP |
| OMT | - |
Streptomyces sp. KCTC 0041BP |
| regiospecific flavonoid 4'OMT | - |
Streptomyces sp. KCTC 0041BP |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Streptomyces sp. KCTC 0041BP |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.027 | - |
S-adenosyl-L-methionine | pH 7.5, 37°C | Streptomyces sp. KCTC 0041BP | |
| 0.027 | - |
quercetin | pH 7.5, 37°C | Streptomyces sp. KCTC 0041BP | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Streptomyces sp. KCTC 0041BP |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Streptomyces sp. KCTC 0041BP | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | GerMIII is present on the biosynthetic gene cluster of dihydrochalcomycin, a macrolide antibiotic that is produced by the Streptomyces sp. KCTC 0041BP. Based on its dependency on the metallic cofactor requirement for its reaction, the enzyme resembles the Class-II plant OMTs | Streptomyces sp. KCTC 0041BP |
| additional information | a double bond between the C2 and the C3 and a single-ring-appended conjugate-hydroxyl group are crucial for the favorable enzymatic conversions of the GerMIII catalysis, modeling and molecular docking | Streptomyces sp. KCTC 0041BP |
| physiological function | the methylation at the 4'-hydroxyl group of the B-ring of flavonoids confers a structural constraint for autooxidation, importance of the regiospecific 4'-OMT | Streptomyces sp. KCTC 0041BP |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.166 | - |
quercetin | pH 7.5, 37°C | Streptomyces sp. KCTC 0041BP | |
| 14.75 | - |
S-adenosyl-L-methionine | pH 7.5, 37°C | Streptomyces sp. KCTC 0041BP | |
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