Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activity is increased, in both in vitro and in vivo carnation tissues, by the inoculation with Fusarium oxysporum f. sp. dianthi | Dianthus caryophyllus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | 1 mM, 40% residual activity | Dianthus caryophyllus | |
Mn2+ | 1 mM, 79% residual activity | Dianthus caryophyllus | |
phenylmercuric acetate | 1 mM, 20% residual activity | Dianthus caryophyllus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
kaempferol | pH 7.0, 25°C | Dianthus caryophyllus | |
0.0033 | - |
apigenin | pH 7.0, 25°C | Dianthus caryophyllus | |
0.011 | - |
4'-hydroxyflavanone | pH 7.0, 25°C | Dianthus caryophyllus | |
0.0735 | - |
genistein | pH 7.0, 25°C | Dianthus caryophyllus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | 45000 | gel filtration and PAGE | Dianthus caryophyllus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dianthus caryophyllus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Dianthus caryophyllus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + a 4'-hydroxyflavanone = S-adenosyl-L-homocysteine + a 4'-methoxyflavanone | kinetic analysis indicates a Ping-Pong mechanism and excludes the formation of a ternary complex | Dianthus caryophyllus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19590 | - |
pH 7.0, 25°C | Dianthus caryophyllus |
Storage Stability | Organism |
---|---|
-20°C, two weeks, about 50% loss of activity | Dianthus caryophyllus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4'-hydroxyflavanone + S-adenosyl-L-methionine | - |
Dianthus caryophyllus | 4'-methoxyflavanone + S-adenosyl-L-homocysteine | - |
? | |
apigenin + S-adenosyl-L-methionine | - |
Dianthus caryophyllus | acacetin + S-adenosyl-L-homocysteine | - |
? | |
genistein + S-adenosyl-L-methionine | - |
Dianthus caryophyllus | biochanin A + S-adenosyl-L-homocysteine | - |
? | |
kaempferol + S-adenosyl-L-methionine | - |
Dianthus caryophyllus | kaempferide + S-adenosyl-L-homocysteine | - |
? | |
additional information | enzyme specifically methylates the hydroxy substituent in 4'-position of the flavones, flavanones and isoflavones in the presence of S-adenosyl-L-methionine. No activity towards hydroxycinnamic acid derivatives | Dianthus caryophyllus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 43000-45000, SDS-PAGE | Dianthus caryophyllus |
Synonyms | Comment | Organism |
---|---|---|
F 4'-OMT | - |
Dianthus caryophyllus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Dianthus caryophyllus | isoelectric focusing | - |
4.2 |