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Literature summary for 2.1.1.231 extracted from

  • Curir, P.; Lanzotti, V.; Dolci, M.; Dolci, P.; Pasini, C.; Tollin, G.
    Purification and properties of a new S-adenosyl-L-methionine:flavonoid 4'-O-methyltransferase from carnation (Dianthus caryophyllus L.) (2003), Eur. J. Biochem., 270, 3422-3431.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information activity is increased, in both in vitro and in vivo carnation tissues, by the inoculation with Fusarium oxysporum f. sp. dianthi Dianthus caryophyllus

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide 1 mM, 40% residual activity Dianthus caryophyllus
Mn2+ 1 mM, 79% residual activity Dianthus caryophyllus
phenylmercuric acetate 1 mM, 20% residual activity Dianthus caryophyllus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
kaempferol pH 7.0, 25°C Dianthus caryophyllus
0.0033
-
apigenin pH 7.0, 25°C Dianthus caryophyllus
0.011
-
4'-hydroxyflavanone pH 7.0, 25°C Dianthus caryophyllus
0.0735
-
genistein pH 7.0, 25°C Dianthus caryophyllus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000 45000 gel filtration and PAGE Dianthus caryophyllus

Organism

Organism UniProt Comment Textmining
Dianthus caryophyllus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Dianthus caryophyllus

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + a 4'-hydroxyflavanone = S-adenosyl-L-homocysteine + a 4'-methoxyflavanone kinetic analysis indicates a Ping-Pong mechanism and excludes the formation of a ternary complex Dianthus caryophyllus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19590
-
pH 7.0, 25°C Dianthus caryophyllus

Storage Stability

Storage Stability Organism
-20°C, two weeks, about 50% loss of activity Dianthus caryophyllus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4'-hydroxyflavanone + S-adenosyl-L-methionine
-
Dianthus caryophyllus 4'-methoxyflavanone + S-adenosyl-L-homocysteine
-
?
apigenin + S-adenosyl-L-methionine
-
Dianthus caryophyllus acacetin + S-adenosyl-L-homocysteine
-
?
genistein + S-adenosyl-L-methionine
-
Dianthus caryophyllus biochanin A + S-adenosyl-L-homocysteine
-
?
kaempferol + S-adenosyl-L-methionine
-
Dianthus caryophyllus kaempferide + S-adenosyl-L-homocysteine
-
?
additional information enzyme specifically methylates the hydroxy substituent in 4'-position of the flavones, flavanones and isoflavones in the presence of S-adenosyl-L-methionine. No activity towards hydroxycinnamic acid derivatives Dianthus caryophyllus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 43000-45000, SDS-PAGE Dianthus caryophyllus

Synonyms

Synonyms Comment Organism
F 4'-OMT
-
Dianthus caryophyllus

pI Value

Organism Comment pI Value Maximum pI Value
Dianthus caryophyllus isoelectric focusing
-
4.2