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Literature summary for 2.1.1.230 extracted from
- Overexpression of the thiostrepton-resistance gene from Streptomyces azureus in Escherichia coli and characterization of recognition sites of the 23S rRNA A1067 2'-methyltransferase in the guanosine triphosphatase center of 23S ribosomal RNA (1994), Eur. J. Biochem., 224, 431-437.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Streptomyces azureus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Protein L11 | binding of either thiostrepton or protein L11 inhibits methylation | Streptomyces azureus | |
| S-Adenosyl-D-homocysteine | competitivewith respect to S-adenosyl-L-methionine | Streptomyces azureus |  |
| S-adenosyl-L-homocysteine | competitive product inhibition | Streptomyces azureus | |
| Thiostrepton | binding of either thiostrepton or protein L11 inhibits methylation | Streptomyces azureus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
adenosine in an RNA fragment containing nucleotides 1029-1122 of the 23S ribosomal RNA from Escherichia coli | pH 7.5, 35°C | Streptomyces azureus | |
| 0.1 | - |
S-adenosyl-L-methionine | pH 7.5, 35°C | Streptomyces azureus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + adenosine1067 in 23S rRNA | Streptomyces azureus | Streptomyces azureus is the producer of the peptide antibiotic thiostrepton. Thiostrepton inhibits protein synthesis by binding to the complex of 23S rRNA and protein L-11 which blocks processes associated with the GTP-hydrolysis center of the ribosome including the binding of guanine nucleotides, elongation factor proteins and tRNA. 23S rRNA (adenosine1067-2'-O)-methyltransferase confers resistance to tthiostrepton | S-adenosyl-L-homocysteine + 2'-O-methyladenosine1067 in 23S rRNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces azureus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces azureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + adenosine in an RNA fragment containing nucleotides 1029-1122 of the 23S ribosomal RNA from Escherichia coli | - |
Streptomyces azureus | S-adenosyl-L-homocysteine + 2'-O-methyladenosine in an RNA fragment containing nucleotides 1029-1122 of the 23S ribosomal RNA from Escherichia coli | - |
? | |
| S-adenosyl-L-methionine + adenosine1067 in 23S rRNA | Streptomyces azureus is the producer of the peptide antibiotic thiostrepton. Thiostrepton inhibits protein synthesis by binding to the complex of 23S rRNA and protein L-11 which blocks processes associated with the GTP-hydrolysis center of the ribosome including the binding of guanine nucleotides, elongation factor proteins and tRNA. 23S rRNA (adenosine1067-2'-O)-methyltransferase confers resistance to tthiostrepton | Streptomyces azureus | S-adenosyl-L-homocysteine + 2'-O-methyladenosine1067 in 23S rRNA | - |
? | |
| S-adenosyl-L-methionine + adenosine1067 in 23S rRNA | RNA sequence variants of the RNA fragment with mutations in nucleotides 1051-1108 are tested as substrates for the methylase. Methylation is dependent on the secondary structure of the hairpin including nucleotide A1067 and the exact sequence U(1066)-A(1067)-G(1068)-A(1O69)-A(1070) of the single strand | Streptomyces azureus | S-adenosyl-L-homocysteine + 2'-O-methyladenosine1067 in 23S rRNA | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Streptomyces azureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Streptomyces azureus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
S-adenosyl-L-homocysteine | pH 7.5, 35°C | Streptomyces azureus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | Streptomyces azureus is the producer of the peptide antibiotic thiostrepton. Thiostrepton inhibits protein synthesis by binding to the complex of 23S rRNA and protein L-11 which blocks processes associated with the GTP-hydrolysis center of the ribosome including the binding of guanine nucleotides, elongation factor proteins and tRNA. 23S rRNA (adenosine1067-2'-O)-methyltransferase confers resistance to thiostrepton | Streptomyces azureus |
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